Instrumentation

Browse by system: Confocal microscopes | Multiphoton system | Widefield microscopes | Computer workstations

Coming soon: tiling/mosaic capabilities on the multiphoton!

Recent additions: A fast and sensitive FV3000RS galvano and resonant scanning confocal, a color and fluorescence MVX10 macroscope, a new brightfield & widefield HCA-compatible Ti2 microscope, and two powerful analysis workstations!


Confocals

 

Zeiss LSM 780
inverted confocal microscope with a motorized stage.
Location: Rm. 8122 NRB


Z-stack acquisition | Mosaic imaging

Lasers Objectives


10x/0.30
EC Plan-Neofluar
WD = 5.2 mm

20x/0.8
Plan-Apochromat
WD = 0.55 mm

40x/1.3
EC Plan-Neofluar (oil) DIC WD = 0.21 mm

40x/1.4
Plan-Apochromat (oil) DIC WD = 0.21 mm

63x/1.40
Plan-Apochromat (oil) DIC
WD = 0.19 mm


Detectors

3 fluorescence
spectral detection: 2 flanking PMTs and the central 34-channel GaAsP detector with ~40% quantum efficiency
1 transmission
brightfield, DIC

405 nm
Diode Laser
DAPI, Hoechst 33258, Alexa 405
458/488/514 nm
Multiline Argon laser
458 nm: CFP
488 nm: FITC, Alexa 488, CY2, GFP, Bodipy, Fluo-4, Calcein
514 nm: YFP
561 nm
Diode laser
Cy3, RFP, Alexa 546, Alexa 561, dsRed2
594 nm
Diode laser
Alexa 594, mCherry
633 nm
Helium-Neon laser
Cy5, DRAQ5

 

Zeiss LSM 710
upright confocal microscope with a motorized stage.
Location: Rm. 8104 NRB


Z-stack acquisition | Mosaic imaging | FRAP

Lasers Objectives


10x/0.30
EC Plan-Neofluar
WD = 5.2 mm

20x/0.8
Plan-Apochromat
WD = 0.55 mm

40x/1.3
EC Plan-Neofluar (oil) DIC WD = 0.21 mm

63x/1.40
Plan-Apochromat (oil) DIC
WD = 0.19 mm


Detectors

3 fluorescence
spectral detection
1 transmission
brightfield, DIC

405 nm
Diode Laser
DAPI, Hoechst 33258, Alexa 405
458/488/514 nm
Multiline Argon laser
458 nm: CFP
488 nm: FITC, Alexa 488, CY2, GFP, Bodipy, Fluo-4, Calcein
514 nm: YFP
543 nm
Helium-Neon laser
Cy3, RFP, Alexa 546, Alexa 561
633 nm
Helium-Neon laser
Cy5, DRAQ5

 


Multiphoton microscope

 


Widefield microscopes

 


 


Computer workstations