Instrumentation

Browse by system: Confocal microscopes | Multiphoton system | Widefield microscopes

Confocals

Zeiss LSM 710 upright confocal microscope with a motorized stage (installed in December 2010) Location: Rm. 8104 NRB Z-stack acquisition \| Mosaic imaging \| FRAP
	Lasers		Objectives 10x/0.30 EC Plan-Neofluar WD = 5.2 mm 20x/0.8 Plan-Apochromat WD = 0.55 mm 40x/1.3 EC Plan-Neofluar (oil) DIC WD = 0.21 mm 63x/1.40 Plan-Apochromat (oil) DIC WD = 0.19 mm Detectors 3 fluorescence spectral detection 1 transmission brightfield, DIC
		405 nm Diode Laser DAPI, Hoechst 33258, Alexa 405
		458/488/514 nm Multiline Argon laser 458 nm: CFP 488 nm: FITC, Alexa 488, CY2, GFP, Bodipy, Fluo-4, Calcein 514 nm: YFP
		543 nm Helium-Neon laser Cy3, RFP, Alexa 546, Alexa 561
		633 nm Helium-Neon laser Cy5, DRAQ5

Olympus FV1000 inverted microscope with a motorized stage and incubator Location: Rm. 8104 NRB Z-stack acquisition \| Mosaic imaging \| Photobleaching \| Time-lapse live cell multiposition imaging
	Lasers		Objectives 10x/0.4 UPLSAPO WD = 3.1 mm 20x/0.45 LUCPLFLN WD = 6.6 - 7.8 mm 40x/0.6 LUCPLFLN WD = 2.7 - 4 mm 60x/1.42 PLAPON WD = 0.15 mm Detectors 3 fluorescence spectral detection 1 transmission brightfield, DIC
		405 nm Diode Laser DAPI, Hoechst 33258, Alexa 405
		458/488/514 nm Multiline Argon laser 458 nm CFP 488 nm: FITC, Alexa 488, CY2, GFP, Bodipy, Fluo-4, Calcein 514 nm YFP
		543 nm Helium-Neon laser Cy3, RFP, Alexa 546, Alexa 561
		633 nm Helium-Neon laser Cy5, DRAQ5

Zeiss LSM 510 upright confocal microscope with a motorized stage Location: Rm. 8122 NRB Z-stack acquisition \| Mosaic imaging \| FRAP
	Lasers		Objectives 10x/0.3 Plan-Neofluar WD = 5.5 mm 10x/0.45 Plan-Apochromat WD = 2.0 mm 20x/0.8 Plan-Apochromat WD = 0.55 mm 40x/1.3 EC Plan-Neofluar (oil) WD = 0.21 mm 63x/1.4 Plan-Apochromat (oil) WD = 0.19 mm Detectors 3 fluorescence spectral detection 1 transmission brightfield, DIC
		458/488/514 nm Multiline Argon laser 458 nm CFP 488 nm: FITC, Alexa 488, CY2, GFP, Bodipy, Fluo-4, Calcein 514 nm YFP
		543 nm Helium-Neon laser Cy3, RFP, Alexa 546, Alexa 561
		633 nm Helium-Neon laser Cy5, DRAQ5

Leica TCS SL inverted confocal microscope with a motorized stage and incubator Location: Rm. 8104 NRB Z-stack acquisition \| Mosaic imaging \| FRAP \| Time-lapse live cells imaging
	Lasers		Objectives 10x/0.30 HC PL Fluotar PH1 WD = 11 mm 20x/0.40 N Plan L WD = 10.8 mm 20x/0.70 HC PlanApo PH2 WD = 0.59 mm 40x/0.80 HCX PlanApo Corr WD = 0.21 mm 63x/1.4 - 0.60 HCX PlanApo Oil WD = 0.1 Detectors 3 fluorescence spectral detection 1 transmission brightfield, DIC
		458/488/514 nm Multiline Argon laser 458 nm CFP 488 nm: FITC, Alexa 488, CY2, GFP, Bodipy, Fluo-4, Calcein 514 nm YFP
		543 nm Helium-Neon laser Cy3, RFP, Alexa 546, Alexa 561
		633 nm Helium-Neon laser Cy5, DRAQ5

Multiphoton microscope

Zeiss LSM 7MP fixed stage upright multiphoton microscope optimized for in vivo imaging. Equipped with anaesthetic vaporizer machine Location: Rm. 8122 NRB Z-stack acquisition \| Deep imaging
	Laser	Objectives 10x/0.3 EC Plan Neofluoar WD = 5.2 mm 20x/1.0 W-Plan Apo (water) WD = 1.8 mm 63x/1.0 W-PlanApo (water) WD = 2.1 mm Detectors BiG assembly: 2 GaAsP detectors
	Coherent Verdi S Titanium:Sapphire Tunable: 690-1050 nm Repetition: 80MHz Pulse width: 70 fs

Widefield microscopes

Nikon Eclipse 80i upright microscope with automated scanning for large area real-time mosaic imaging. Controlled by Surveyor software. Location: Rm. 8122 NRB Z-stack acquisition \| Mosaic imaging \| FRAP
	Filters			Objectives 4x/0.75 PlanFluor WD = 17.1 mm 10x/0.3 PlanFluor WD = 16 mm 20x/0.5 Plan Fluor WD = 2.1 mm 40x/0.75 Plan Fluor WD = 0.66 mm Detector Qimaging Retiga Exi Data Sheet Stage Bioprecision stage with stepper motor and XYZ joystick
		No 1., Chroma 49006, Ex. 350/60, Em. 460/50 DAPI, Hoechst 33258, Alexa 405
		No. 2, Chroma 49002, Ex. 470/40, Em. 525/50 GFP, Alexa 488, FITC, Cy2
		No. 3, Chroma 49008, Ex. 560/40, Em. 630/75 mCherry, dsRed, Alexa 568, Alexa 594, Cy3, mRFP1, DyLight 594, Mito Tracker 633
		No. 4, Chroma 51005 designed to attenuate the typically bright PI emission and balance it with the emission of FITC.
		No. 5. Chroma 49008, Ex. 620/60, Em. 700/75 Alexa 647, Cy5, Draq5, DyLight 649
		No. 6. Empty, Use for brightfield imaging