Instrumentation

Browse by system: Confocal microscopes | Multiphoton system | Widefield microscopes

Confocals

Zeiss LSM 710
upright confocal microscope with a motorized stage (installed in December 2010)
Location: Rm. 8104 NRB


Z-stack acquisition | Mosaic imaging | FRAP

Lasers Objectives


10x/0.30
EC Plan-Neofluar 
WD = 5.2 mm

20x/0.8
Plan-Apochromat 
WD = 0.55 mm

40x/1.3
EC Plan-Neofluar (oil)  DIC WD = 0.21 mm

63x/1.40
Plan-Apochromat (oil)  DIC
WD = 0.19 mm


Detectors


3
fluorescence
spectral detection
1 transmission
brightfield, DIC

405

405 nm
Diode Laser
DAPI, Hoechst 33258, Alexa 405
ML-Argon 458/488/514 nm
Multiline Argon laser
458 nm: CFP
488 nm: FITC, Alexa 488, CY2, GFP, Bodipy, Fluo-4, Calcein
514 nm: YFP

543 nm
Helium-Neon laser
Cy3, RFP, Alexa 546, Alexa 561

633 nm
Helium-Neon laser
Cy5, DRAQ5

 

 

 

 


Multiphoton microscope

 


Widefield microscopes