The sources of image degradation can be divided into four independent phenomena: noise, scatter, glare, and blur. While all of these apply for widefield microscopy, application of a pinhole eliminates almost all sources of degradation except noise.
Deconvolution is a computationally intensive image processing technique that is being increasingly utilized for improving the contrast and resolution of digital images captured in the microscope. It is capable of:
A few packages are available that perform deconvolution. Huygens from SVI (Scientific Volume Imaging) is the most popular one. A number of algorithms allows to address images acquired at different conditions and delivers superior quality. It is especially useful for the groups interested in visualizing small structures - microtubules, spines, receptors, etc.
Object analyzer tool for 3D microscopy images in Huygens Professional allows you to obtain statistics of individual objects by clicking on them, or analyzing all objects with a single button press.
When and Where
Oct. 20, 2012 - Nov. 8, 2012
Rm. 8122, Data Analysis Station
How to Apply
Please contact Vladimir Ghukasyan to reserve time and get a training/help with deconvolution/object analysis.
Rm. 7109F NRB
Tel.: 919 966 5807
Additional Information:Deconvolution in Optical Microscopy: Tutorial