Resources
Zeiss LSM 510
- fixed stage upright microscope
- two lasers
Argon: 458/476/488/514 nm
488 nm: FITC, Alexa 488, CY2, GFP, Bodipy, Fluo-4, Calcein
458 nm CFP
514 nm YFP
HeNe1: 543 nm
CY3, Texas Red, Alexa 568, Alexa 594, Rhodamine, TRITC, DsRed, Alexa 543
Coherent Titanium:Sapphire Ultrafast Infrared Laser: tunable
Excites fluorophores in the FITC to YFP range.
458 nm CFP
514 nm YFP
HeNe1: 543 nm
CY3, Texas Red, Alexa 568, Alexa 594, Rhodamine, TRITC, DsRed, Alexa 543
Coherent Titanium:Sapphire Ultrafast Infrared Laser: tunable
Excites fluorophores in the FITC to YFP range.
Contact Person: Dr. Robert E. Peterson
Leica TCS SL
- inverted microscope with motorized stage and heated chamber
- three lasers: allow for triple labeling
- "Blue" Argon: 458/476/488/514 nm
488 nm: FITC, Alexa 488, CY2, GFP, Bodipy, Fluo-4, Calcein - "Green" Neon Helium: 543 nm
CY3, Texas Red, Alexa 568, Alexa 594, Rhodamine, TRITC, DsRed, Alexa 543 - "Red" Neon Helium: 633 nm
CY5, Alexa 633, Alexa 647
- "Blue" Argon: 458/476/488/514 nm
- spectral scan head
Contact: Dr. Franck Polleux
Nikon-Based Surveyor System with TurboScan Software for Fast-Mosaic Scanning
- Nikon 80i Research Upright Microscope
- Surveyor/TurboScan Software, Oasis-blue Controller Blue Expio Module for real-time seamless tiling at 5X, 10X, 20X, 40X
- Bioprecision stage with stepper motor and XYZ joystick
- Chroma Technology Photofluor Light Source for even illumination of fluorophores excited by blue, green, red and far-red wavelengths of light
- Qimaging Retiga Exi high-speed cooled color digital camera system
Contact: Dr. Robert E. Peterson
UNC Neuroscience Center - UNC School of Medicine