The primary goal of this core is to assist neuroscientists at UNC-Chapel Hill in their efforts to molecularly manipulate and study nervous system function. This Core is directed by Dr. JrGang Cheng, a pioneer in the field of BAC recombineering.
We offer the following services to UNC neuroscientists on a fee-for-service basis:
- Generates transgenic and gene-targeting constructs using Bacterial Artificial Chromosome (BAC) recombineering.
- Assists neuroscientists in generating knockin/out mice by screening gene targeted embryonic stem (ES) cells (includes 5’ and 3’ probe generation and Southern blot screening).
- Generates expression and recombinant adenoviral (rAAV) plasmid constructs.
- Prepares massively parallel (next-generation) sequencing libraries (RNA-seq, ChIP-seq).
Location & Hours
Neuroscience Research Building
115 Mason Farm Road, Campus Box 7250
Chapel Hill, NC