Fees

 

Service

NINDS and UNC Neuroscience PIs

Other UNC PIs

Outside UNC Projects

Construct design1

$150/hr

$150/hr

$150/hr

Sequence verification2

$10

$10

$10

 

 

 

 

Recombineering

 

 

 

BAC clone acquisition

Market cost

Market cost

Market cost

Knockout/in or BAC construct

$1000

$2000

$3600

Site-directed mutagenesis on plasmid

$450

$900

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Targeting cassette (< 1kb arms)

$500 (Human)

$400 (Mouse)

$1000

$800

$2000

$1200

 

 

 

 

Plasmid construction3

 

 

 

Simple, one ligation construct4

$150

$180

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Low copy plasmid construct4

$180

$210

 

rAAV construct

$180

$210

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Synthesis DNA

Market cost

Market cost

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1Billed if project prematurely terminated by PI.  Not billed if construct completed and purchased.  2One sequence reaction with common primer. 3Additional costs might be charged such as sequencing, primers synthesis and uncommon restriction enzyme.  4For more complex constructs, the cost will be $150 (or $180 for low copy plasmid) per ligation+transformation+plasmid prep.  For example, a construct that requires sequential insertion of two different pieces of DNA will cost 2 x $150, plus any associated sequencing costs.

 

Note: This charge table is regarding fees and should act as a general guideline. An estimated cost will be given to each PI after construct design has been completed by the Core Director.  Once PI agrees regarding design and pricing, construct making will be initiated. When a construct is not stable, or the map and sequence are not correct (unrelated to an error by the Core), the Core Director will ask the PI if he/she wishes terminate this specific project or if the PI wants the Core to continue with various trouble-shooting efforts at additional expenses  (to the PI).  If project is terminated, PI will be charged for the cost of materials used up to the termination point. If the PI chooses to continue, a new cost estimate will be given to the PI by the Core Director.  PI must agree to pay for the additional costs prior to moving forward.  Please feel free to contact the Core Director with any questions. It is always a pleasure and free of charge to discuss a specific project.