The primary goal of this core is to assist neuroscientists at UNC-Chapel Hill in their efforts to molecularly manipulate and study nervous system function.  This Core is directed by Dr. JrGang Cheng, a pioneer in the field of BAC recombineering. Our Facility has been in operation for more than 10 years and has made many complex gene targeting constructs for use in mice. We have also generated constructs expressing Cre recombinase, Tet on/off trans-activators and optogenetic proteins to study neural circuit function. We can also use recombineering, including CRISPR-Cas9 genome editing, to generate various mutations at the gene level including: exon swaps, duplications, truncations, deletions, point mutations and conditional gene modifications.


We offer the following services at an affordable cost to UNC neuroscientists:


Generates transgenic and gene-targeting constructs and modified cell lines using CRISPR-Cas9 system (fee-for-service).

Generates transgenic and gene-targeting constructs using Bacterial Artificial Chromosome (BAC) recombineering (fee-for-service).

Generates expression plasmids, recombinant adenoviral (rAAV) or other viral based plasmid constructs or any other type of plasmid construct (fee-for-service).

Provide neuroscientists with access to a quantitative PCR (Q-PCR) machine (usage fee applies).


We provide free initial consultation in the design of transgenic or gene targeting constructs and plasmids.