Stephani Page

Stephani Page
Undergraduate Institution: North Carolina A&T State University
Department: Biochemistry & Biophysics
PI: Bob Bourret
Research Summary:

Microorganisms use two-component systems to mediate responses to stimuli. The canonical components of two-component systems are the sensor kinase and the response regulator. Receiver domains of response regulators contain the site of phosphorylation, which mediates responses by switching output function on and off. Receiver domains have highly conserved active site residues that catalyze phosphorylation and dephosphorylation. Despite the conserved active site and similarities in structure and sequence amongst receiver domains, self-catalyzed dephosphorylation of receiver domains reportedly occurs over a nearly million-fold range of reaction rates. I want to understand what factors influence receiver domain autodephosphorylation rates and the mechanisms by which those factors exert influence.