Purity of Product

These oligonucleotides are provided by following deprotecting and drying steps.  In general, most users can use these oligonucleotides after resuspension with TE buffer (or double distilled water) and quantification, but the following simple extraction methods can clean up the organic materials produced during synthesis:

1. Resuspend the DNA in 500 ul TE buffer and vortex.
2. Transfer the DNA solution to an eppendorf tube.
3. Add 400 ul chloroform and vortex.
4. Centrifuge the tube at 3000-5000 rpm for 5 minutes.
5. Take the upper DNA aqueous solution, without disturbing the chloroform interface.
6. Speed-vacuum dry the DNA solution for 1 minute to evaporate any remaining chloroform.
7. Measure the OD at A260.