Xinchun Pi, PhD
Dr. Pi studies the molecular mechanisms underneath the regulation of endothelial cell functions during vascular development and a variety of pathological conditions such as inflammation, atherosclerosis, peripheral vascular disease, cancer, diabetes and obesity. Her particular focus is to understand the signaling pathways associated with endothelial function that are critical in order to develop treatments for these vascular-associated conditions. Particular attentions are directed toward LRP1 (low density lipoprotein receptor-related protein 1) – and Bmper (Bmp-binding endothelial cell precursor-derived regulator)-dependent signaling pathways. Her work focuses on understanding how endothelial cells respond to ligand, initiate signaling cascades and cellular events such as proliferation, migration, differentiation, angiogenesis, inflammation and atherosclerosis, and change the metabolism to satisfy their needs at different active status.
Pi X (Corresponding author), Xie L, Portbury AL, Kumar S, Lockyer P, Li X, Patterson C. NADPH oxidase- generated ROS are required for SDF-1α-stimulated angiogenesis. Arterioscler Thromb Vasc Biol. Accepted.
Dyer LA, Pi X, Patterson C. The role of BMPs in endothelial cell function and dysfunction. (2014) Trends Endocrinol Metab. Jun 4. pii: S1043-2760(14)00085-X. PMID: 24908616
Pi X (Co-corresponding author), Schmitt CE, Xie L, Portbury AL, Wu Y, Lockyer P, Dyer LA, Moser M, Bu G, Flynn EJ 3rd, Jin SW, Patterson C (2012) An LRP1-dependent endocytic mechanism governs the signaling output of the bmp system in endothelial cells and in angiogenesis. Circulation Research. 111(5):564-74. PMID: 22777006
Xie L, Pi X, Mishra A, Fong G, Peng J, Patterson C (2012) PHD3-dependent hydroxylation of HCLK2 promotes the DNA damage response. The Journal of Clinical Investigation. 122(8):2827-36. PMID: 22797300
Pi X, Lockyer P, Dyer L, Schisler J, Carey S, Sweet D, Chen Z, Tzima E, Willis M, Homeister J, Moser M, Patterson C (2012) Bmper inhibits endothelial inflammation and protects against atherosclerosis. Arteriosclerosis, Thrombosis, and Vascular Biology. 32(9):2214-22. PMID: 22772758
Bode M, Wu Y, Pi X, Lockyer P, Dechyapirom W, Portbury AL, Patterson C (2011) Regulation of ankyrin repeat and suppressor of cytokine signaling box protein 4 expression in the immortalized murine endothelial cell lines MS1 and SVR: a role for tumor necrosis factor alpha and oxygen. Cell Biochemistry and Function. 29(4):334-41. PMID: 21506136
Aitsebaomo J, Srivastava S, Zhang H, Jha S, Wang Z, Winnik S, Veleva AN, Pi X, Lockyer P, Faber JE, Patterson C. Recombinant human interleukin-11 treatment enhances collateral vessel growth after femoral artery ligation. Arterioscler Thromb Vasc Biol. 2011 Feb;31(2):306-12
Pi X, Wu Y, Ferguson JE 3rd, Portbury AL, Patterson C. SDF-1alpha stimulates JNK3 activity via eNOS-dependent nitrosylation of MKP7 to enhance endothelial migration. Proc Natl Acad Sci USA. 2009 Apr 7;106(14):5675-80.
Kelley R, Ren R, Pi X, Wu Y, Moreno I, Willis M, Moser M, Ross M, Podkowa M, Attisano L, Patterson C. A concentration-dependent endocytic trap and sink mechanism converts Bmper from an activator to an inhibitor of Bmp signaling. J Cell Biol. 2009 Feb 23;184(4):597-609.
Pi X, Patterson C. Twin layers of lightning: a new role for the chaperone Hsp90 in angiogenesis. Arterioscler Thromb Vasc Biol. 2008 Jan;28(1):6-7.
Pi X, Ren R, Kelley R, Zhang C, Moser M, Bohil A, DiVito M, Cheney RE, Patterson C. Sequential roles for Myosin-X in BMP6-dependent filopodial extension, migration, and activation of BMP receptors. J Cell Biol. 2007 Dec 31; 179(7):1569-82
Wang H, Charles PC, Wu Y, Ren R, Pi X, Moser M, Barshishat-Kupper M, Rubin JS, Perou C, Bautch V, Patterson C. Gene expression profile signatures indicate a role for Wnt signaling in endothelial commitment from embryonic stem cells. Circ Res. 2006;98(10):1331-1339.
Pi X, Garin G, Xie L, Zheng Q, Wei H, Abe J, Yan C, Berk BC. BMK1/ERK5 is a novel regulator of angiogenesis by destabilizing hypoxia inducible factor 1alpha. Circ Res. 2005;96(11):1145-1151.
Kim D, Aizawa T, Wei H, Pi X, Rybalkin S, Berk BC, Yan C. Angiotensin II increases phosphodiesterase 5A expression in vascular smooth muscle cells: a mechanism by which angiotensin II antagonizes cGMP signaling. J Mol Cell Cardiol. 2005;38(1):175-84.
Pi X, Yan C, Berk BC. Big mitogen-activated protein kinase (BMK1)/ERK5 protects endothelial cells from apoptosis. Circ Res. 2004;94(3):362-369.
Pi X, Yan C, Berk BC. Atheroprotective mechanisms of flow: inhibition of apoptosis. (Review) International congress series. Atherosclerosis XIII. Proceedings of the 13th international atherosclerosis symposium. 2004;1262C:129-132.
Pi X, Yan C, Kim D, Chen J, Berk BC. Differential expression of genes from nitrate-tolerant rat aorta. J Vasc Res. 2002 Jul-Aug;39(4):304-10.
Kim D, Rybalkin SD, Pi X, Wang Y, Zhang C, Munzel T, Beavo JA, Berk BC, Yan C. Upregulation of phosphodiesterase 1A1 expression is associated with the development of nitrate tolerance. Circulation. 2001 Nov 6;104(19):2338-43.
Surapisitchat J, Hoefen RJ, Pi X, Yoshizumi M, Yan C, Berk BC. Fluid shear stress inhibits TNF-alpha activation of JNK but not ERK1/2 or p38 in human umbilical vein endothelial cells: Inhibitory crosstalk among MAPK family members. Proc Natl Acad Sci U S A. 2001 May 22;98(11):6476-81.