Hahn lab biosensors and caged proteins are available at “Addgene”, a nonprofit repository of constructs (http://www.addgene.org/Klaus_Hahn). Addgene produces the constructs with good quality control. We can synthesize dyes on a collaborative basis, and they will soon be commercially available. Please let Dr. Hahn know if there is any way we can help.

Below are links and information that will assist with the production and use of biosensors and caged proteins and with imaging. Good luck!

Imaging Protocols, Biosensor Production & Software

RhoA Biosensor; single chain, genetically encoded FRET
RhoA protocol [.doc]
Link to original publication, Pertz, et al, 2006.

Cdc42 Biosensor; dye-labeled WASP fragment, for endogenous Cdc42
Cdc42 dye-based biosensor protocol [.doc]
Link to original publication, Nalbant et al., 2004
Link to Cdc42 Biosensor Methods paper

Photobleaching Corrections (specific for Cdc42 but applicable to other sensors)
Link to photobleach correction paper and associated program files

Rac Biosensors
Note re: Rac Biosensors

Image-based autofocus
Link to article and associated computer files.



Labeling proteins and peptides with dyes
Dye labeling protocol [pdf]
Site-specific attachment of two different dyes to proteins and peptides:
Bark and Hahn, Methods, 2000 [pdf]



Spectral data for dye test kit
Dye data for dye kit [doc] or [pdf]