Hahn lab biosensors are available at “Addgene”, a nonprofit repository of
constructs (http://www.addgene.org/Klaus_Hahn). Addgene produces the
constructs with good quality control. Please let us know if there are any
problems or if Dr. Hahn can help in some way.

Below are links to protocols and papers that will assist with the production
and use of different biosensors. Good luck!



Biosensors
Photoactivatable proteins
Others

RhoA Biosensor; single chain, genetically encoded FRET
RhoA protocol [.doc]
Link to original publication, Pertz, et al, 2006.

Cdc42 Biosensor; dye-labeled WASP fragment
Cdc42 dye-based biosensor protocol [.doc]
Link to original publication, Nalbant et al., 2004
Link to Cdc42 Biosensor Methods paper

Photobleaching Corrections (specific for Cdc42 but applicable to other sensors)
Link to photobleach correction paper and associated program files

Rac Biosensors
Note re: Rac Biosensors

Software-based autofocus
Link to article and associated computer files.



Labeling proteins and peptides with dyes
Dye labeling protocol [pdf]
Site-specific attachment of two different dyes to proteins and peptides: Bark and Hahn, Methods, 2000 [pdf]



Spectral data for dye test kit
Dye data for dye kit [doc] or [pdf]