Hahn Lab - Publications

Publications

Patents

Publications

Garrett, S.C., Hodgson, L., Rybin, A., Toutchkine, A., Hahn, K.M., Lawrence, D.S., Bresnick, A.R. A Biosensor of S100A4
Metastasis Factor Activation - Inhibitor Screening and Cellular Activation Dynamics. Biochemistry 47:986-96, 2008.
Click here for abstract.

Hodgson, L. and Hahn, K.M. Design and optimization of genetically encoded fluorescent biosensors: GTPase
biosensors. Methods in Cell Biol. 85:63-81, 2008. Click here for abstract.

Cai, X., Lietha, D., Ceccarelli, D.F., Karginov, A.V., Rajfur, Z., Jacobson, K., Hahn, K.M., Eck, M.J., Schaller, M.D. Spatial and
Temporal Regulation of FAK Activity in Living Cells. Mol Cell Biol., 2007 Oct 29; [Epub ahead of print]. Click here for abstract.

Toutchkine, A., Han, W.G., Ullmann, M., Liu, T., Bashford, D., Noodleman, L., and Hahn, K.M. Experimental and DFT studies:
novel structural modifications greatly enhance the solvent sensitivity of live cell imaging dyes. J Phys Chem A.
111:10849-60, 2007. Click here for abstract.

Healy, K.D., Hodgson, L., Kim, T.Y., Shutes, A., Maddileti, S., Juliano, R.L., Hahn, K.M., Harden, T.K., Bang, Y.J., Der, C.J.
DLC-1 suppresses non-small cell lung cancer growth and invasion by RhoGAP-dependent and independent
mechanisms. Mol Carcinog. 2007 Oct 11; [Epub ahead of print]. Click here for abstract.

Malone, M.H., Sciaky, N., Stalheim, L., Hahn, K.M., Linney, E., Johnson, G.L. Laser-scanning velocimetry: A confocal
microscopy method for quantitative measurement of cardiovascular performance in zebrafish embryos and larvae.
BMC Biotechnology, 7:40, 2007. Click here for abstract.

Frantz, C., Karydis1, A., Nalbant, P., Hahn, K. M., and Barber, D. L. Positive feedback between Cdc42 activity and H+
efflux by the Na-H exchanger NHE1 for polarity of migrating cells. J. Cell Biol. 179: 403-410, 2007. Click here for abstract.

El-Sibai, M., Nalbant, P., Sarmiento, C., Macaluso, F., Cammer, M, Condeelis, J.S., Hahn, K.M. and Backer, J.M. Cdc42 is
Required for EGF-Stimulated Protrusion and Motility in Carcinoma Cells. J. Cell Science, 120(Pt 19): 3465-74, 2007.
Click here for abstract.

Hodgson, L., Chan, E.W.L., Hahn, K.M., and Yousaf, M.N. Combining Surface Chemistry with a FRET-Based Biosensor to
Study the Dynamics of RhoA GTPase Activation in Cells on Patterned Substrates. J. Am. Chem. Soc. 129:9264 - 9265,
2007. Click here for abstract.

Toutchkine, A., Nguyen, D.V., and Hahn, K.M. Merocyanine Dyes with Improved Photostability. Org. Lett. 9(15)2775-2777,
2007. Click here for abstract.

Toutchkine, A., Nguyen, D.V., and Hahn, K.M. Simple one-pot preparation of water-soluble, cysteine- reactive cyanine
and merocyanine dyes for biological imaging. Bioconjugate Chem.18(4):1344-1348, 2007. Click here for abstract.

Birkenfeld, J., Nalbant, P., Bohl, B.P., Pertz, O.P., Hahn, K.M., and Bokoch, G.M. GEF-H1 modulates localized RhoA
activation during cytokinesis under the control of mitotic kinases. Developmental Cell.12(5): 699-712, 2007.
Click here for abstract.

Van Keymeulen, A., Wong, K., Knight, Z.A., Govaerts, C., Hahn, K.M., Shokat, K.M., and Bourne, H.R. To stabilize neutrophil
polarity, PIP3 and Cdc42 augment RhoA activity at the back as well as signals at the front. J. Cell Biol., 174:437-445,
2006. Click here for abstract.

Hodgson, L. Nalbant, P. Shen, F., and Hahn, K. Imaging and photobleach correction of Mero-CBD, sensor of endogenous
Cdc42 activation. Methods Enzymol., 406:140-156, 2006. Click here for abstract.

Shen, F., Hodgson, L., and Hahn, K. Digital Autofocus Methods for Automated Microscopy. Meth. Enzymol., 414: 620-632,
2006. Click here for abstract.

Wang, Y.L. Hahn, K.M., Murphy, R.F., and Horwitz, A.F. From Imaging to understanding: Frontiers in Live Cell Imaging,
Bethesda, MD, April 19-21, 2006. J. Cell Biol., 174(4):481-484, 2006. Click here for abstract.

Shen, F., L. Hodgson, A. Rabinovich, O. Pertz, K.M. Hahn, and J.H. Price. Functional Proteomics for Cell Migration.
Cytometry, 69(7):563-782, 2006. Click here for abstract.

Pertz, O., L. Hodgson, R. R. Klemke, and K.M. Hahn. Spatio-temporal dynamics of RhoA activity in migrating cells.
Nature, 440(7087):1069-1072, 2006. Click here for abstract.

Nakamura K, Uhlik MT, Johnson NL, Hahn KM, G. L. Johnson. PB1 Domain-Dependent Signaling Complex Is Required for
Extracellular Signal-Regulated Kinase 5 Activation. Mol Cell Biol., 26(6):2065-79. 2006. Click here for abstract.

Wong K., Pertz O., Hahn K. M., H.. Bourne. Neutrophil polarization: Spatiotemporal dynamics of RhoA activity support a
self-organizing mechanism. Proc Natl Acad Sci USA., 103(10):3639-44, 2006. Click here for abstract.

Hodgson L, Nalbant P, Shen F, Hahn K. Imaging and Photobleach Correction of Mero-CBD, Sensor of Endogenous Cdc42
Activation. Methods Enzymol., 406:140-56, 2006. Click here for abstract.

Subauste, M., P. Nalbant, E. Adamson, K.M. Hahn. Vinculin Controls PTEN Protein Level by Maintaining the Interaction
of the Adherens Junction Protein a-Catenin with the Scaffolding Protein MAGI-2. J. Biol. Chem., 280 (7):5676-81, 2005.
Click here for abstract.

Subauste, M., O. Pertz, E. Adamson, C.E. Turner, S. Junger, K.M. Hahn. Vinculin Modulation of Paxillin-FAK Interactions
Regulates ERK to Control Survival and Motility. J Cell Biol., 165:171-181, 2004. Click here for abstract.

Nalbant, P., L. Hodgson, V. Kraynov, A. Toutchkine, K.M. Hahn. Activation of Endogenous Cdc42 Visualized in Living
Cells. Science, 305: 1615-1619, 2004. Click here for abstract.

Liu, T., W. Han, F. Himo, G.M. Ullmann, D. Bashford, A. Toutchkine, K.M. Hahn, L. Noodleman. Density Functional Vertical
Self-Consistent Reaction Field Theory for Solvatochromism Studies of Solvent-Sensitive Dyes. J. Phys. Chem. A
108:3545-3555, 2004. Click here for abstract.

Pertz,O. and K.M. Hahn. Designing biosensors for Rho family proteins - deciphering the dynamics of Rho family
GTPase activation in living cells. J. Cell Sci. 117(8): 1313-1318,2004. Click here for abstract.

Tan, X., P. Nalbant, A. Toutchkine, D. Hu, E.R. Vorpagel, K.M. Hahn, and H.P. Lu. Single Molecule Study of Protein-Protein
Interaction Dynamics in a Cell Signaling System. J. Phys. Chem. B 108: 737-744, 2004. Click here for abstract.

Han, W.G., T. Liu, F. Himo, A. Toutchkine, D. Bashford, K. M. Hahn, and L. Noodleman. A Theoretical Study of the UV
Absorption and Emission Solvatochromic Properties of Solvent Sensitive Dyes. ChemPhysChem, 4: 1084-1094, 2003.
Click here for abstract.

Toutchkine, A., V. Kraynov, and K.M. Hahn. Solvent-Sensitive Dyes to Report Protein Conformational Changes in Living
Cells. J. Amer. Chem. Soc., 125:4132-4145, 2003. Click here for abstract.

Gaits, F. and K.M. Hahn. Shedding Light on Cell Signaling: Interpretation of FRET Biosensors, Sci. STKE 2003, p3, 2003
Click here for abstract.

Price, J., A. Goodacre, K.M. Hahn, L. Hodgson, E. Hunter, S. Krajewski, R.F. Murphy, A. Rabinovich, J.C. Reed, and S.
Heynen. Advances in Molecular Labeling, High Throughput Imaging and Machine Intelligence Portend Powerful
Functional Cellular Biochemistry Tools. J. Cell.Biochem. 39:194-210, 2003. Click here for abstract.

Shine, S.T., J.W. Wen, W. Yung, P. Nalbant, K.M. Hahn, and R.A. Cerione. Anti-apoptotic Cdc42 Mutants are Potent
Activators of Cellular Transformation. Biochemistry, 41: 12350-12358, 2002. Click here for abstract.

Gardiner, E.M., N. Pestonjamasp, B.P. Bohl, C. Chamberlain, K.M. Hahn and G.M.Bokoch. Spatial and Temporal Analysis of
Rac Activation During Live Neutrophil Chemotaxis: Roles in Leading Edge Extension and Tail Retraction. Current
Biology, 12: 2029-2034, 2002. Click here for abstract.

Katsumi, A., J. Milanini, W.B. Kiosses, M.A. del Pozo, R. Kaunas, S. Chien, K.M. Hahn, and M.A. Schwartz. Effects of Cell
Tension on Membrane Protrusion Through Regulation of the GTPase Rac. J.Cell Biol., 158: 1-13, 2002. Click here for abstract.

Nishimura, N., H. Pluttner, K.M. Hahn, and W.E. Balch. The g Subunit of AP-3 is Required for Efficient Transport of VSV-G
from the Trans-Golgi Network to the Cell Surface. Proc. Natl. Acad. Sci., USA, 99: 6755-6760, 2002. Click here for abstract.

Toutchkine, A., Nalbant, P. and Hahn, K.M. Facile synthesis of thiol-reactive CY3 and CY5 derivatives with enhanced
water solubility. Bioconjugate Chemistry, 3: 387-391, 2002. Click here for abstract.

Toutchkine, A. and K.M. Hahn. Live cell fluorescent biosensors for activated signaling proteins. Curr. Op Cell Biol.
14:167-172, 2002. Click here for abstract.

Del Pozo, M.A., W.B. Kiosses, N.B. Alderson, N. Meller, K.M. Hahn, and A. Schwartz. Integrin-regulated membrane
translocation of GTP-Rac induces localized interaction with effectors due to dissociation of RhoGDI, Nature Cell
Biology, 4:232-239, 2002. Click here for abstract.

Frosst, P., Guan, T., Subauste,C., Hahn, K., and Gerace, L. Tpr is localized within the nuclear basket of the pore complex
and has a role in nuclear protein export. J. Cell Biol.,156(4):617-30, 2002. Click here for abstract.

Subauste, M.C., B. List, X. Guan, K.M. Hahn, R. Lerner, and B. Gilula. A catalytic antibody produces fluorescent tracers of
gap junction communication in living cells. J. Biol. Chem., 276:49164-49168, 2001.Click here for abstract.

Weiss, T.S., C.E. Chamberlain, T. Takeda, P. Lin, K.M. Hahn and M.G. Fraquhar. Galpha binding to calnuc on Golgi
membranes in living cells monitored by fluorescence resonance energy transfer of green fluorescent tracers of gap
junction communication in living cells. J. Biol. Chem., 276:49164-49168, 2001. Click here for abstract.

Kiosses, W. B., Hahn, K. M., Giannelli, G., and Quaranta, V. Characterization of laminin-5 morphological and cytoskeletal
changes in MCF10A breast epithelial cells. Cell Adhes.commun., 8:29-44, 2001.

Kraynov, V. S., Chamberlain, C. E., Bokoch, G. M., Schwartz, M. A., Slabaugh, S., and K. M. Hahn. Localized Rac activation
dynamics visualized in living cells. Science, 290:333-337, 2000. Click here for abstract.

Chester Chamberlain and Klaus M. Hahn. Watching Proteins in the Wild: Flourescence Methods to Study Protein
Dynamics in Living Cells. Traffic, 1 (10), 755-762., 2000. Click here for abstract.

Chamberlain, C.E., Kraynov, V., & Hahn, K.M. Imaging Spatiotemporal Dynamics of Rac Activation in vivo with FLAIR.
Methods in Enzymology, 325, 389-400, 2000. Click here for pdf of article.

Bark, S. J., Schmid, S., and K. M. Hahn. A highly efficient method for site-specific modification of peptides after
chemical synthesis. J. Am. Chem. Soc., 122 (15): 3567-3573, 2000. Click here for abstract.

M.C. Subauste, M.V. Herrath, C.E. Chamberlain, T.H. Chuang, G.M. Bokoch, and K.M. Hahn. Rho family proteins modulate
CTL and Fas-induced apoptosis through control of the actin cytoskeleton. J. Biol. Chem., 275: 9725-33, 2000. Click here
for abstract.

Bark, S. J., and K. M. Hahn. Fluorescent indicators of peptide cleavage in the trafficking compartments of living cells:
Peptides Site-Specifically labeled with two dyes. Methods, 20: 429-435, 2000. Click here for abstract.

D.G. Stupack, E. Li, J.A. Kehler, R.L. Geahlen, K. Hahn, G.R. Nemerow, and D.A. Cheresh. Matrix valency regulates
integrin-mediated lymphoid adhesion via Syk kinase. J. Cell Bio., 144: 77-787, 1999. Click here for abstract.

Nishimura, N., S. Bannykh, S. Slabaugh, J. Matteson, K.M. Hahn, and W.E. Balch. The Di-Acidic (DxE) Code Found in the
Cytoplasmic Domain of Cargo Directs Concentration During Export from the Endoplasmic Reticulum. J. Biol. Chem.,
274: 15937-15946, 1999. Click here for abstract.

K.M. Hahn, J. Kolega, J. Montibeller, R. DeBiasio, P. Post, J. Myers and D.L. Taylor. Fluorescent analogs: Optical
biosensors of the chemical and molecular dynamics of macromolecules in living cells, In Fluorescent and Luminescent
Probes for Biological Activity. Second Edition. Mason, ed., Academic Press, London, 554-566. 1999.

G. Ben-Ami, V. Ozeri, E. Hanski, F. Hofmann, K. Aktories, K.M. Hahn, G.M. Bokoch, and I. Rosenshine. Agents that inhibit
Rho, Rac and Cdc42 do not block formation of actin pedestals in HeLa cells infected with enteropathogenic
Escherichia coli. Infect. Immun., 66: 1755-1758, 1998. Click here for abstract.

Tsung-Hsien Chuang, K.M. Hahn, J.D. Lee, D.E. Danley, and G.M. Bokoch. The Small GTPase Cdc42 Initiates an Apoptotic
Signaling Pathway in Jurkat T Lymphocytes. Molecular Biology of the Cell., 8: 1687-1698, 1997. Click here for abstract.

K.A. Giuliano, P.L. Post, K.M. Hahn, D.L. Taylor. Fluorescent Protein Biosensors: Measurement of Molecular Dynamics in
Living Cells. In The Cytoskeleton. Spudich, ed. Annual Reviews Inc. 107-136, 1996.

R.D. Shelby, K.M. Hahn, and K.F. Sullivan. Dynamic elastic behavior of alpha-satellite DNA domains visualized in situ in
living human cells. J.Cell Biol., 135: 545-557, 1996. Click here for abstract.

V.W. Cornish, K.M. Hahn, and P.G. Schultz. Site-specific protein modification using a ketone handle. J.Am Chem. Soc.
118: 8150-8151, 1996. Click here for abstract.

J.B. Waters, M.B.A. Oldstone and K.M. Hahn. Changes in the cytoplasmic structure of cytotoxic T lymphocytes during
target cell recognition and killing. J.Immunol., 157:3396-3403, 1996. Click here for abstract.

P.A. Knauf, F.Y. Law, and K.M. Hahn. An oxonol dye is the most potent known inhibitor of band 3-mediated anion
exchange. Am. J. Physiol (Cell Physiol.), C1073-C1077, 1995. Click here for abstract.

K. Giuliano, P.L. Post, K.M. Hahn, and D.L. Taylor. Fluorescent protein biosensors: measurement of molecular dynamics
in living cells. Ann. Rev. Biophys. Biomol. Struct. 24:405434, 1995. Click here for abstract.

H. Lewicki, A. Tishon, P. Borrow, C.F. Evans, J.E. Gairin, K.M. Hahn, D.A. Jewell, I.A. Wilson, and M.B.A. Oldstone. CTL
escape viral variants. I. Generation and molecular characterization. Virology, 210:2940, 1995. Click here for abstract.

I.C. Bark, K.M. Hahn, A.E. Ryabinin, and M.C. Wilson. Differential expression of SNAP-25 protein isoforms during
divergent vesicle fusion events of neuronal development. Proc. Natl Acad.Sci USA 92:1510-1514, 1995.
Click here for abstract.

P. Southwick, K.M. Hahn, J. Chao, P. Parry, A.S. Wagman, M. Wagner and A.S. Waggoner. A long wavelength biolabeling
reagent based on the oxonol flurophore. J. Fluorescence 5(2):231-235, 1995. Click here for abstract.

K.M. Hahn, R. DeBiasio, A. Tishon, H. Lewicki, J.E. Gairin, G. LaRocca, D.L. Taylor, and M.B.A. Oldstone. Antigen
presentation and cytotoxic T lymphocyte killing studied in individual, living cells. Virology 20l:330-340, 1994.
Click here for abstract.

K.M. Hahn, P.A. Conrad, J. Chao, D.L. Taylor and A.S. Waggoner. A photocrosslinking, fluorescent indicator of
mitochondrial membrane potential. J. Histochem. Cytochem. 41:631-634, 1993. Click here for abstract.

K.M. Hahn, R. DeBiasio and D.L. Taylor. Patterns of elevated free calcium and calmodulin activation in living cells.
Nature 359:736-738, 1992. Click here for abstract.

K.M. Hahn, S.B. Hastie and R.J. Sundberg. Synthesis and evaluation of 2 diazo-3,3,3-trifluoropropanoyl derivatives of
colchicine and podophyllotoxin as photoaffinity labels: reactivity, photochemistry, and tubulin binding. Photochem.
Photobiol 55:17-27, 1992. Click here for abstract

K.M. Hahn and P.L. Southwick. A stable diazo photoaffinity label with increased absorptivity and effective
photoactivation beyond 300 nm. Anal Biochem. 196:271-278, 1991. Click here for abstract.

K.M. Hahn, W.G. Humphries, A.M. Helms, S.B. Hastie and T.L. Macdonald. Structural requirements for the binding of
colchicine analogs to tubulin: the role of the C-1O substituent. Biomed Chem. Lett.1:471-476, 1991.
Click here for abstract.

K.M. Hahn, A.S. Waggoner and D.L. Taylor. A calcium-sensitive fluorescent analog of calmodulin based on a novel
calmodulin-binding fluorophore. J. Biol Chem. 265:20335-20345, 1990. Click here for abstract.

Questions or Comments? Contact Hahn Lab.
Hahn Lab, UNC Chapel Hill, Department of Pharmacology