Johnson Lab at the UNC Department of Pharmacology

Publications

Journal Highlights


JBC CoverCover of Journal of Cell Biology April 16, 2010
"Rho kinase inhibition rescues the endothelial cell cerebral cavernous malformation phenotype."

About the cover: Cerebral cavernous malformations (CCM) are vascular lesions causing seizure and stroke. Mutations in one of three genes, ccm1, ccm2, or ccm3, cause an increase in RhoA expression and activation. Increased RhoA and Rho kinase activity inhibits endothelial cell vessel-like tube formation. A small molecule inhibitor of Rho kinase rescues the CCM endothelial cell phenotype, defining Rho kinase as a therapeutic target to rescue endothelial cells from loss of CCM protein function. For details see the article by Borikova et al., pages 11760–11764 (online at JBC).

Selected Recent Publications

  • Nakamura, K., and Johnson, G.L. (2010) Activity assays for extracellular signal-regulated kinase 5. Methods Mol. Biol. 661:91-106. PMID: 20811978. abstract
  • Konhilas, J.P., Boucek, D.M., Horn, T.R., Johnson, G.L., and Leinwand, L.A. (2010) The Role of MEKK1 in Hypertrophic Cardiomyopathy. Int. Heart J. 51(4):277-84. PMID: 20716846. abstract
  • Dibble, C.F., Horst, J.A., Malone, M.H., Park, K., Temple, B., Cheeseman, H., Barbaro, J.R., Johnson, G.L., and Bencharit, S. (2010) Defining the functional domain of programmed cell death 10 through its interactions with phosphatidylinositol-3,4,5-trisphosphate. PLoS One 5(7):e11740.  PMID: 20668527. PMC2909203. abstract
  • Borikova, A.L., Dibble, C.F., Sciaky, N., Welch, C.M., Abell, A.N., Bencharit, S. and Johnson, G.L., (2010) Rho kinase inhibition rescues the endothelial cell cerebral cavernous malformation phenotype. (cover) J. Biol. Chem. 285:11760-11764. PMID: 20181950. PMC2852911. abstract
  • Nakamura, K., Kimple, .A.J., Siderovski, .D.P. and Johnson G.L. (2010) PB1 domain interaction of  p62/Sequestosome 1 and MEKK3 regulates NFkB activation. J. Biol. Chem. 285: 2077-2089. PMID: 19903815. PMC2804364. abstract
  • Leblanc GG, Golanov E, Awad IA, Young WL; Biology of Vascular Malformations of the Brain NINDS Workshop Collaborators. (2009) Biology of vascular malformations of the brain. Stroke. 40(12):e694-702. Review.PMID: 19834013. PMC2810509. abstract
  • Crose, L.E., Hilder, T.L., Sciaky, N., and Johnson, G.L. (2009) Cerebral cavernous malformation 2 protein promotes smad ubiquitin regulatory factor 1-mediated RhoA degradation in endothelial cells. J. Biol. Chem. 284(20):13301-5. PMID: 19318350. PMC2679429. abstract
  • Xie, L., Jing, L., Yu, Y., Nakamura, K., Parker, C.E., Johnson, G.L., and Chen, X. (2009)  In vivo profiling endogenous interactions with knock-out in mammalian cells. Anal. Chem. 81(4):1411-7.PMID: 19199569. abstract
  • Frye, S.V. and Johnson, G.L. Inhibitors paradoxically prime kinases. (2009) Nature Chem. Biol. News & Views. 5:448-449. PMID: 19465930. online article
  • Machacek, M., Hodgson, L., Welch, C., Elliott, H, Pertz, O., Naban P., Abell, A., Johnson, G.L., Hahn, K.L., and Danuser, G. (2009) Coordination of Rho GTPase activities during cell protrusion. Nature 461, 99-103. PMID: 1969301. PMC2885353. abstract
  • Abell, A. N., Granger, D.A., Johnson, N.L., Vincent-Jordan, N., Dibble, C.F. and Johnson, G.L.. (2009)    Trophoblast stem cell maintenance by FGF4 requires MEKK4 activation of JNK. Mol. Cell. Biol. 29:2748-61. PMID: 19289495. PMC2682043. abstract

For a more complete listing of publications click on the pubmed link below.

 

 

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