Pharmacology Student Publications 2010-2011

Alan JK, Berzat AC, Dewar BJ, Graves LM, Cox AD. Regulation of the Rho family small GTPase Wrch-1/RhoU by C-terminal tyrosine phosphorylation requires Src. Mol Cell Biol. 2010 Sep;30(17):4324-38. Epub 2010 Jun 14.

González-Pérez V, Reiner DJ, Alan JK, Mitchell C, Edwards LJ, Khazak V, Der CJ, Cox AD. Genetic and functional characterization of putative Ras/Raf interaction inhibitors in C. elegans and mammalian cells. J Mol Signal. 2010 Feb 23;5:2.

Bauman JA, Kole R. Modulation of RNA splicing as a potential treatment for cancer. Bioeng Bugs. 2011 May-Jun;2(3):125-8. Epub 2011 May 1

Bauman JA, Li SD, Yang A, Huang L, Kole R. Anti-tumor activity of splice-switching oligonucleotides. Nucleic Acids Res. 2010 Dec;38(22):8348-56. Epub 2010 Aug 18.

Bosch DE, Wittchen ES, Qiu C, Burridge K, Siderovski DP. Unique Structural and Nucleotide Exchange Features of the Rho1 GTPase of Entamoeba histolytica. J Biol Chem. 2011 Nov 11;286(45):39236-46. Epub 2011 Sep 19.

Kimple AJ, Bosch DE, Giguère PM, Siderovski DP. Regulators of G-protein signaling and their Gα substrates: promises and challenges in their use as drug discovery targets. Pharmacol Rev. 2011 Sep;63(3):728-49. Epub 2011 Jul 7.

Kosloff M, Travis AM, Bosch DE, Siderovski DP, Arshavsky VY. Integrating energy calculations with functional assays to decipher the specificity of G protein-RGS protein interactions. Nat Struct Mol Biol. 2011 Jun 19;18(7):846-53.

Sammond DW, Bosch DE, Butterfoss GL, Purbeck C, Machius M, Siderovski DP, Kuhlman B. Computational design of the sequence and structure of a protein-binding peptide. J Am Chem Soc. 2011 Mar 30;133(12):4190-2. Epub 2011 Mar 9.

Bosch DE, Kimple AJ, Sammond DW, Muller RE, Miley MJ, Machius M, Kuhlman B, Willard FS, Siderovski DP. Structural determinants of affinity enhancement between GoLoco motifs and G-protein alpha subunit mutants. J Biol Chem. 2011 Feb 4;286(5):3351-8. Epub 2010 Nov 29.

Borikova AL, Dibble CF, Sciaky N, Welch CM, Abell AN, Bencharit S, Johnson GL. Rho kinase inhibition rescues the endothelial cell cerebral cavernous malformation phenotype. J Biol Chem. 2010 Apr 16;285(16):11760-4. Epub 2010 Feb 24.

Cappell KM, Larson B, Sciaky N, Whitehurst AW. Symplekin specifies mitotic fidelity by supporting microtubule dynamics. Mol Cell Biol. 2010 Nov;30(21):5135-44. Epub 2010 Sep 7.

Whitehurst AW, Xie Y, Purinton SC, Cappell KM, Swanik JT, Larson B, Girard L, Schorge JO, White MA. Tumor antigen acrosin binding protein normalizes mitotic spindle function to promote cancer cell proliferation. Cancer Res. 2010 Oct 1;70(19):7652-61. Epub 2010 Sep 28.

Torres MP, Clement ST, Cappell SD, Dohlman HG. Cell cycle-dependent phosphorylation and ubiquitination of a G protein alpha subunit. J Biol Chem. 2011 Jun 10;286(23):20208-16. Epub 2011 Apr 26.

Cappell SD, Dohlman HG. Selective regulation of MAP kinase signaling by an endomembrane phosphatidylinositol 4-kinase. J Biol Chem. 2011 Apr 29;286(17):14852-60. Epub 2011 Mar 9.

Cappell SD, Baker R, Skowyra D, Dohlman HG. Systematic analysis of essential genes reveals important regulators of G protein signaling. Mol Cell. 2010 Jun 11;38(5):746-57.

Dibble CF, Horst JA, Malone MH, Park K, Temple B, Cheeseman H, Barbaro JR, Johnson GL, Bencharit S. Defining the functional domain of programmed cell death 10 through its interactions with phosphatidylinositol-3,4,5-trisphosphate. PLoS One. 2010 Jul 23;5(7):e11740.

Yadav PN, Kroeze WK, Farrell MS, Roth BL. Antagonist functional selectivity: 5-HT2A serotonin receptor antagonists differentially regulate 5-HT2A receptor protein level in vivo. J Pharmacol Exp Ther. 2011 Oct;339(1):99-105. Epub 2011 Jul 7.

Yadav PN, Abbas AI, Farrell MS, Setola V, Sciaky N, Huang XP, Kroeze WK, Crawford LK, Piel DA, Keiser MJ, Irwin JJ, Shoichet BK, Deneris ES, Gingrich J, Beck SG, Roth BL. The presynaptic component of the serotonergic system is required for clozapine's efficacy. Neuropsychopharmacology. 2011 Feb;36(3):638-51. Epub 2010 Nov 3.

Dong S, Allen JA, Farrell M, Roth BL. A chemical-genetic approach for precise spatio-temporal control of cellular signaling. Mol Biosyst. 2010 Aug;6(8):1376-80. Epub 2010 Jun 7. Review.

Abbas AI, Urban DJ, Jensen NH, Farrell MS, Kroeze WK, Mieczkowski P, Wang Z, Roth BL. Assessing serotonin receptor mRNA editing frequency by a novel ultra high-throughput sequencing method. Nucleic Acids Res. 2010 Jun;38(10):e118. Epub 2010 Feb 25.

Farrell M, Roth BL. Allosteric antipsychotics: m4 muscarinic potentiators as novel treatments for schizophrenia. Neuropsychopharmacology. 2010 Mar;35(4):851-2.

Goldsmith JR, Uronis JM, Jobin C. Mu opioid signaling protects against acute murine intestinal injury in a manner involving Stat3 signaling. Am J Pathol. 2011 Aug;179(2):673-83. Epub 2011 Jun 13.

Gresset A, Hicks SN, Harden TK, Sondek J. Mechanism of phosphorylation-induced activation of phospholipase C-gamma isozymes. J Biol Chem. 2010 Nov 12;285(46):35836-47. Epub 2010 Aug 31.

Jordan NV, Johnson GL, Abell AN. Tracking the intermediate stages of epithelial-mesenchymal transition in epithelial stem cells and cancer. Cell Cycle. 2011 Sep 1;10(17):2865-73. Epub 2011 Sep 1.

Huang W, Umbach DM, Vincent Jordan N, Abell AN, Johnson GL, Li L. Efficiently identifying genome-wide changes with next-generation sequencing data. Nucleic Acids Res. 2011 Oct 1;39(19):e130. Epub 2011 Jul 29.

Abell AN, Jordan NV, Huang W, Prat A, Midland AA, Johnson NL, Granger DA, Mieczkowski PA, Perou CM, Gomez SM, Li L, Johnson GL. MAP3K4/CBP-regulated H2B acetylation controls epithelial-mesenchymal transition in trophoblast stem cells. Cell Stem Cell. 2011 May 6;8(5):525-37.

Nakamura K, Kimple AJ, Siderovski DP, Johnson GL. PB1 domain interaction of p62/sequestosome 1 and MEKK3 regulates NF-kappaB activation. J Biol Chem. 2010 Jan 15;285(3):2077-89. Epub 2009 Nov 10.

Neel NF, Martin TD, Stratford JK, Zand TP, Reiner DJ, Der CJ. The RalGEF-Ral Effector Signaling Network: The Road Less Traveled for Anti-Ras Drug Discovery. Genes Cancer. 2011 Mar;2(3):275-87.

Martin TD, Samuel JC, Routh ED, Der CJ, Yeh JJ. Activation and involvement of Ral GTPases in colorectal cancer. Cancer Res. 2011 Jan 1;71(1):206-15.

Kidd AR 3rd, Snider JL, Martin TD, Graboski SF, Der CJ, Cox AD. Ras-related small GTPases RalA and RalB regulate cellular survival after ionizing radiation. Int J Radiat Oncol Biol Phys. 2010 Sep 1;78(1):205-12. Epub 2010 Jul 7.

Wang Q, Zimmerman EI, Toutchkine A, Martin TD, Graves LM, Lawrence DS. Multicolor monitoring of dysregulated protein kinases in chronic myelogenous leukemia. ACS Chem Biol. 2010 Sep 17;5(9):887-95.

Mitchell AM, Nicolson SC, Warischalk JK, Samulski RJ. AAV's anatomy: roadmap for optimizing vectors for translational success. Curr Gene Ther. 2010 Oct 1;10(5):319-40.

Kovarik ML, Gach PC, Ornoff DM, Wang Y, Balowski J, Farrag L, Allbritton NL. Micro Total Analysis Systems for Cell Biology and Biochemical Assays. Anal Chem. 2011 Oct 21. [Epub ahead of print]

Hodges-Loaiza HB, Parker LE, Cox AD. Prenylation and Phosphorylation of Ras Superfamily Small GTPases. In: The Enzymes Volume 30, Protein Prenylation, Part B, eds. Tamanoi F, Hrycyna CA, Bergo MO. Academic Press/Elsevier. In press.

Waldo GL, Ricks TK, Hicks SN, Cheever ML, Kawano T, Tsuboi K, Wang X, Montell C, Kozasa T, Sondek J, Harden TK. Kinetic scaffolding mediated by a phospholipase C-beta and Gq signaling complex. Science. 2010 Nov 12;330(6006):974-80. Epub 2010 Oct 21.

Rogan SC, Roth BL. Remote control of neuronal signaling. Pharmacol Rev. 2011 Jun;63(2):291-315. Epub 2011 Mar 17. Review.

Krashes MJ, Koda S, Ye C, Rogan SC, Adams AC, Cusher DS, Maratos-Flier E, Roth BL, Lowell BB. Rapid, reversible activation of AgRP neurons drives feeding behavior in mice. J Clin Invest. 2011 Apr 1;121(4):1424-8.

Dong S, Rogan SC, Roth BL. Directed molecular evolution of DREADDs: a generic approach to creating next-generation RASSLs. Nat Protoc. 2010;5(3):561-73. Epub 2010 Feb 25.

Singh A, Boyer JL, Der CJ, Zohn IE. Transformation by a nucleotide-activated P2Y receptor is mediated by activation of Galphai, Galphaq and Rho-dependent signaling pathways. J Mol Signal. 2010 5:11.

Asokan A, Conway JC, Phillips JL, Li C, Hegge J, Sinnott R, Yadav S, DiPrimio N, Nam HJ, Agbandje-McKenna M, McPhee S, Wolff J, Samulski RJ. Reengineering a receptor footprint of adeno-associated virus enables selective and systemic gene transfer to muscle. Nat Biotechnol. 2010 Jan;28(1):79-82. Epub 2009 Dec 27.

Stevens EV, Banet N, Onesto C, Plachco A, Alan JK, Nikolaishvili-Feinberg N, Midkiff BR, Kuan PF, Liu J, Miller CR, Vigil D, Graves LM, Der CJ.   RhoGDI2 antagonizes ovarian carcinoma growth, invasion and metastasis. Small GTPases. 2011 2:202-210.

Stevens EV, Wells A, Shin JH, Liu J, Der CJ, Schoenfisch MH. Nitric oxide-releasing silica nanoparticle inhibition of ovarian cancer cell growth. Mol. Pharmaceutics. 2010 7:775-785.

Stevens EV, Der CJ.  Rho history.  In The Rho GTPases in Cancer. (K. Van Golen, ed.). Springer, 2010, Chapter 1, pp. 3-28.

Stratford JK, Bentrem DJ, Anderson JM, Fan C, Volmar KA, Marron JS, Routh ED, Caskey LS, Samuel JC, Der CJ, Thorne LB, Calvo BF, Kim HJ, Talamonti MS, Iacobuzio-Donahue CA, Hollingsworth MA, Perou CM, Yeh JJ. A six-gene signature predicts survival of patients with localized pancreatic ductal adenocarcinoma. PLoS Med. 2010 Jul 13;7(7):e1000307.

Welch CM, Elliott H, Danuser G, Hahn KM. Imaging the coordination of multiple signalling activities in living cells. Nat Rev Mol Cell Biol. 2011 Oct 21;12(11):749-56.

Hinde E, Digman MA, Welch C, Hahn KM, Gratton E. Biosensor Förster resonance energy transfer detection by the phasor approach to fluorescence lifetime imaging microscopy. Microsc Res Tech. 2011 Aug 19.

Koivusalo M, Welch C, Hayashi H, Scott CC, Kim M, Alexander T, Touret N, Hahn KM, Grinstein S. Amiloride inhibits macropinocytosis by lowering submembranous pH and preventing Rac1 and Cdc42 signaling. J Cell Biol. 2010 Feb 22;188(4):547-63. Epub 2010 Feb 15. Erratum in: J Cell Biol. 2010 Apr 19;189(2):385.

Samson T, Welch CM, Monaghan-Benson E, Hahn KM, Burridge K. Endogenous RhoG is Rapidly Activated after EGF Stimulation through Multiple GEFs. Mol Biol Cell. 2010 May; 21(9): 1629-42.

Zand TP, Reiner DJ, Der CJ. Ras effector switching promotes divergent cell fates in C. elegans vulval patterning. Dev Cell. 2011 Jan 18;20(1):84-96.

Beltran AS, Rivenbark AG, Richardson BT, Yuan X, Quian H, Hunt JP, Zimmerman E, Graves LM, Blancafort P. Generation of tumor-initiating cells by exogenous delivery of OCT4 transcription factor. Breast Cancer Res. 2011 Sep 27;13(5):R94. [Epub ahead of print]

Leisewitz AV, Zimmerman EI, Huang M, Jones SZ, Yang J, Graves LM. Regulation of ENT1 expression and ENT1-dependent nucleoside transport by c-Jun N-terminal kinase. Biochem Biophys Res Commun. 2011 Jan 7;404(1):370-5. Epub 2010 Dec 9.

Zimmerman EI, Dollins CM, Crawford M, Grant S, Nana-Sinkam SP, Richards KL, Hammond SM, Graves LM. Lyn kinase-dependent regulation of miR181 and myeloid cell leukemia-1 expression: implications for drug resistance in myelogenous leukemia. Mol Pharmacol. 2010 Nov;78(5):811-7. Epub 2010 Aug 6.