Goicoechea - Images
| Figure 1 |  | Figure 2 |  |
| Palladin localization to PDBu-induced podosomes |
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Figure 1. A7r5 cells were plated on fibronectin and treated with the phorbol ester PDBu. After fixation, endogenous palladin and Eps8was were detected by immunofluorescence using anti-palladin and anti-Eps8 antibodies. |
| Palladin localization to PDGF-induced dorsal ruffles |
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Figure 2. A7r5 cells were plated on fibronectin overnight and serum-starved for 2 hours prior to growth factor treatment. The addition of PDGF led to the induction of dynamic, actin-based membrane protrusions called circular dorsal ruffles. After fixation, endogenous palladin and cortactin were detected in these structures by immunofluorescence using anti-palladin and anti-cortactin polyclonal antobodies. |
