Figure 1. A7r5 cells were plated on fibronectin and treated with the phorbol ester PDBu. After fixation, endogenous palladin and Eps8was were detected by immunofluorescence using anti-palladin and anti-Eps8 antibodies.
Palladin localization to PDGF-induced dorsal ruffles
Figure 2. A7r5 cells were plated on fibronectin overnight and serum-starved for 2 hours prior to growth factor treatment. The addition of PDGF led to the induction of dynamic, actin-based membrane protrusions called circular dorsal ruffles. After fixation, endogenous palladin and cortactin were detected in these structures by immunofluorescence using anti-palladin and anti-cortactin polyclonal antobodies.
