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James ("Jed") Ferguson Pharmacology 103 Mason Farm Road, CB# 7126 Lab Phone: (919) 843-6477 Lab Fax: |
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4th Year IVB Trainee
4th Year Graduate Student
MD/PhD |
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Ph.D. Project Description:
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Targeted protein degradation in Vascular Development | |
Principle Investigator: |
Cam Patterson | |
IVB Collaborator: |
Victoria Bautch | |
Project Description: |
To investigate the biologic significance and mechanism of E3 ligases during vascular development. | |
Rotations: |
Leslie Parise: Characterization of a sindbis virus-mediated gene expression system in megakaryocytes | |
| Cam Patterson: Investigating the role of HB-EGF in thrombin-induced vascular smooth muscle cell proliferation | ||
Familiar Techniques: |
Western Blot, Northern Blot, Southern Blot, RT-PCR, whole mount in situ hybridization, yeast 2-hybrid, immunoprecipitation, GST-fusion protein purification, immunofluorescence/confocal microscopy, knockout, lentivirus-mediated RNAi, dual luciferase reporter assays, anti-peptide polyclonal antibody production, | |
Other Techniques used in Laboratory: |
in vitro ubiquitination assays, microarray, EMSA, embryoid body culture model, embryo culture, animal models | |
Undergraduate degree: |
B.A. in Interdisciplinary (2000) from University of Virginia, Charlottesville, Virginia | |
Other Professional/
Graduate degree(s): |
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Awards/ Scholarships/ Grants/ Fellowships: |
Echols Scholar, UVa 1996-2000, Phi Beta Kappa, 2000, American Heart Association Pre-doctoral Fellow 2005-7 | |
Publications: |
Ferguson JE, 3rd, Patterson C. Break the cycle. Cell Cycle. 2003;2:211-219. | |
| Ferguson JE, 3rd, Kelley, RW, and Patterson, C. Mechanisms of Endothelial Differentiation in Embryonic Vasculogenesis. ATVB. 2005. | ||
Personal Comments: |
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