Imaging

UNC-Chapel Hill is fortunate to have some overlap in key equipment in the imaging/microscopy fields. Listed below are our separate facilities, their equipment, and links to their main websites, most of which include calendars that allow you to schedule time on the equipment. Please check all of the sites that might have the services you need.

Electron Microscopy Facility

Jack Griffith, PhD, Director
http://cancer.med.unc.edu/research/cores/facility.asp?facilityID=8

  • TEM instruments dedicated to imaging nucleic acid complexes and proteins

Cell & Molecular Imaging Facility

Ken Jacobson, PhD, Director
Robert Currin and Gerald Gordon, Managers
http://www-cellbio.med.unc.edu/grad/depttest/cmifhome.htm

  • Biorad MRC 600 laser scanning confocal microscope
  • CARV spinning disc confocal microscope (inverted)
  • Confocal Laser Scanning Microscopy (CLSM)
  • Fluorescence Microscopy
  • Fluorescence Plate Reader-fluorescence, absorbance, lifetime-filter based system (Xenon flash lamp), microinjectors, temperature control
  • FRAP-fluorescence recovery after photobleaching
  • FRET-fluorescence resonance energy transfer
  • Image Processing Workstations-Adobe suite, Metamorph, Imaris 3D software, Volocity 3D software, Zeiss 510 software, Licor Odyssey software
  • Light Microscopy
  • Live cell imaging
  • Microinjection System (Eppendorf)
  • Multiphoton Microscopy
  • Slide scanning, Image Stitching, Cell Classification
  • Zeiss Axiovert 200 inverted microscope-Orca ER camera, x-y stage control, excitation and emission filter wheels
  • Zeiss 410 laser scanning microscope(inverted), 458nm, 488nm, 514nm, 543nm, 643nm laser lines
  • Zeiss 510 NLO Meta laser scanning confocal microscope-Arg/Krypton laser, HeNe laser and Ti-sapphire multiphoton laser, environmental chamber, inverted and upright microscopes, physiology software module

Electron Microscopy

Hal Mekeel, Manager
mekeel@med.unc.edu (919) 968-6868

  • Electron Microscopy
  • JEOL 6300 scanning electron microscope
  • Scanning Electron Microscopy (SEM)
  • Tecnai 12 Transmission Electron Microscope by FEI
  • Transmission Electron Microscopy (TEM)
  • Ultramicrotomy

Microscopy Services Laboratory

Robert Bagnell, PhD, Director
http://www.med.unc.edu/microscopy/

Electron Microscopy

  • Zeiss EM910 Transmission Electron Microscope with a digital camera
  • Zeiss Supra 25 Field Emission Scanning Electron Microscope
  • Specimen preparation services for electron microscopy including immuno-em

Light Microscopy and Macroscopy

1) Wide field instruments -

  • Nikon Microphot FXA Upright Fluorescence Microscope, (bright field, dark field, phase contrast, polarized light, differential interference contrast, epi-fluorescence) with a digital camera
  • Olympus IMT-2 Inverted Fluorescence Microscope, (bright field, phase contrast, epi-fluorescence) with a digital camera
  • Wild M420 Macroscope - for imaging specimens up to 2 cm long, (transmitted and epi-illumination systems) with a digital camera

2) Confocal Laser Scanning instruments -

  • Zeiss LSM-5 Pascal, (2 confocal channels, 1 DIC channel)
  • Olympus FV500, (3 confocal channels, 1 DIC channel, virtual channels) equipped for short-term live cell imaging utilizing the Bioptechs Delta T Live Cell system

3) Live Cell Imaging -

  • Olympus IX70 Inverted Fluorescence Microscope equipped for live cell imaging, (bright field, phase contrast, epi-fluorescence) with a digital camera. This system is enclosed in an environmental chamber and is operated remotely using Improvision’s OpenLab software.

4) Laser Capture Microdissection -

  • Zeiss PALM (bright field, epi-fluorescence)
  • Arcturus PixCell ll, (bright field, phase-contrast, epi-fluorescence)

Image Processing and Measurement

  • Imaris from BitPlane (three and four dimensional reconstruction and measurement)
  • Auto Deblur from Autoquant (deconvolution from confocal and widefield image series)
  • MetaMorph from MMI (morphometry)
  • Fovea Pro from Reindeer Graphics (morphometry)
  • PhotoShop from Adobe (general image processing)

Michael Hooker Microscopy Facility

Michael Chua, PhD, Director
http://mhmicroscopy.med.unc.edu/

  • Leica inverted microscope with fluorescence, transmitted & DIC, color & B/W digital cameras
  • Nikon upright microscope and dissecting scope with 11 MPixel color CCD camera
  • Nikon TE2000 inverted microscope, transmitted/Phase/DIC/fluorescence multifunction time lapse with dual cameras, ratio imaging, Fura-2
  • Zeiss 510 Meta Laser Scanning Confocal Microscope, 458, 488, 543 & 633 nm laser excitation & spectral detectors, high speed z-axis stage, spectral unmixing, transmitted/DIC
  • Leica SP2 AOBS Laser Scanning Confocal Microscope, 351, 364, 458, 488, 512, 561 & 633 nm lasers and adjustable bandwidth detection, 3 channels simultaneous + transmitted/DIC, 9 channels sequential, high speed z-axis stage
  • Spinning Disk (Yokogawa) Confocal Microscope (Perkin Elmer UltraView) 488, 568, 647 nm laser excitation
  • LeicaTCS-4D Laser Scanning Confocal Microscope
  • Leica AS-LMD Laser Micro-Dissection and capture microscope
  • Atomic Force Microscope, Topometrix Explorer
  • Microscope stage heaters
  • Volocity 3-D rendering, 3-D/4-D image analysis, particle tracking, colocalization
  • MetaMorph off line analysis/processing
  • Zeiss AIM confocal analysis, 3-D, FRAP, Coloc, FRET, spectral unmixing
  • Fluorescence microscopy
  • Immunofluorescence imaging
  • Cconfocal laser scanning microscopy
  • Live cell imaging
  • Light microscopy
  • Digital imaging
  • Digital Oscilloscope
  • Laser Power meter
  • Fluorescence Image blind deconvolution
  • Multimeter & clamp on current meter
  • Laser Capture Microdissection
  • Macroscopic imaging
  • Immuno light microscopy methods consulting
  • Light Microscopy training
  • Light Microscopy workshop
  • Light Microscopy seminars