Douglas Phanstiel, PhD

Douglas Phanstiel, PhD

Assistant Professor of Cell Biology & Physiology

3300 Thurston Building
Camus Box 7280
Chapel Hill , NC 27599-7280
919-966-0558


Specialty Areas: Genomics, Proteomics, Bioinformatics, Genomic software development

Chronology:
BS: University of California – San Diego (Cell Biology & Biochemistry), 2001
PhD: University of Wisconsin – Madison (Chemistry), 2011
Post-doctoral fellow: Stanford University (laboratory of Michael P Snyder), 2011-2016
Assistant Professor of Cell Biology & Physiology: University of North Carolina at Chapel Hill, 2016-present

Description of research and/or clinical interests: Dr. Phanstiel’s lab studies transcriptional regulation during innate immune cell development both in healthy individuals and in the context of human disease.  The lab is particularly interested in the mechanisms by which specific intergenic genomic loci, known as enhancers, influence transcriptional changes of genes hundreds of thousands of base pairs away.  To accomplish their work the Phanstiel lab employs interdisciplinary techniques including DNA sequencing, bioinformatics, and genome editing applied to primary human cells and cell culture model systems.

 Selected Publications:

1)    Phanstiel DH*, Van Bortle K*, Spacek D, Hess GT, Shamim MS, Machol I, Love MI, Aiden EL, Bassik MC, Snyder MP. Static and Dynamic DNA Loops form AP-1-Bound Activation Hubs during Macrophage Development. Molecular Cell. 2017 Sep 21;67(6):1037-1048.e6. PubMed PMID: 28890333; PubMed Central PMCID: PMC5610110.

2)    Phanstiel DH, Boyle AP, Heidari N, Snyder MP. Mango: a bias-correcting ChIA-PET analysis pipeline. Bioinformatics. 2015 Oct 1;31(19):3092-8. PubMed PMID: 26034063; PubMed Central PMCID: PMC4592333.

3)    Heidari N*, Phanstiel DH*, He C, Grubert F, Jahanbani F, Kasowski M, Zhang MQ, Snyder MP. Genome-wide map of regulatory interactions in the human genome. Genome Research. 2014 Dec;24(12):1905-17. PubMed PMID: 25228660; PubMed Central PMCID: PMC4248309.

4)    Phanstiel DH, Boyle AP, Araya CL, Snyder MP. Sushi.R: flexible, quantitative and integrative genomic visualizations for publication-quality multi-panel figures. Bioinformatics. 2014 Oct;30(19):2808-10. PubMed PMID: 24903420; PubMed Central PMCID: PMC4173017.

 5)    Phanstiel DH*, Brumbaugh J*, Wenger CD, Tian S, Probasco MD, Bailey DJ, Swaney DL, Tervo MA, Bolin JM, Ruotti V, Stewart R, Thomson JA, Coon JJ. Proteomic and phosphoproteomic comparison of human ES and iPS cells. Nature Methods. 2011 Sep 11;8(10):821-7. PubMed PMID: 21983960; PubMed Central PMCID: PMC3432645.

 6)    Phanstiel D, Brumbaugh J, Berggren WT, Conard K, Feng X, Levenstein ME, McAlister GC, Thomson JA, Coon JJ. Mass spectrometry identifies and quantifies 74 unique histone H4 isoforms in differentiating human embryonic stem cells. Proceedings of the National Academy Sciences. U S A. 2008 Mar 18;105(11):4093-8. PubMed PMID: 18326628; PubMed Central PMCID: PMC2393763

Awards and Honors
2015:  K99/R00 Pathway to Independence
2012:  Damon Runyon Fellowship Award

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