Equipment

Sequencers:

  • 454 GS Junior : ~500 bp read-length Next-Gen Sequencer
  • Ion Torrent PGM : ~200-400 bp read-length Next-Gen Sequencer
  • Ion Torrent S5: ~200bp read-length Next-Gen Sequencer

Robots:

  • Tecan Evo 50: Robot with 96 well; pipetting head for post-PCR clean-up
  • Tecan Evo 150: Robot with integrated 384 well Roche LC480 real-time PCR reactions
  • CAS-1200 (Corbett Res.): Robot pipetting robot which pipettes 96/384 well PCR reactions with 0.1 microL accuracy (aka QIAgility)

Supporting Equipment:

  • Ion Chef for automated AmpliSeq library to chip loading for Ion Torrent sequencers
  • Roche LightCycler 480 (384-well and 96-well)
  • Centrifuge for 96 and 384 well plate centrifugation
  • PIPPIN Prep automated gel fragments sizing and elution station
  • Agilent Bionanalyzer 2100
  • Caliper GX 384 well analyzer
  • Tecan M200 fluorometer with NanoDrop capability
  • Qubit 3.0 DNA analyzer (Life Technologies)
  • Digital PCR machine (ABI/Life Technologies)

Servers and Computers:

  • Penguin Computing Tempest 4400 computing server
  • Department-provided FTP server for data delivery
  • Multiple Apple Mac Pro workstations, including bioinformatics software, for analysis

Example usage

This video published by the Journal of Visualized Experiments shows how samples may be set up and aliquoted by the Tecan Freedom Evo®, as well as quantified by the Roche Lightcycler® 480 II.

Chugh P, Tamburro K, Dittmer DP (2010). Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR (qPCR) Arrays. J Vis Exp. 46.

http://www.jove.com/index/details.stp?id=2210, doi: 10.3791/2210