qPCR requests should be submitted to Dr. Dirk Dittmer and/or the Vironomics Core Manager, email is best. Once approved, samples should be prepared following the SOP of qPCR mRNA Preparation.
454 GS Junior Sequencing
- Please contact Core Manager for availability.
- Samples must meet below requirements or they are subject to rejection.
- NanoDrop is not compatible for assessing sequencing samples. If you do need to use the NanoDrop, please compensate for the overestimation and send 1ug.
- Amplicons: This technology is sensitive to primer-dimers, please contact Core Manager for Roche protocol of removing small fragments using AMPure beads.
*Please note that the Vironomics Core offers the PicoGreen reagents and fluorometer as part of the sequencing service. We also have an Agilent for assessing base-pair size.