qPCR requests should be submitted to Dr. Dirk Dittmer and/or the Vironomics Core Manager, email is best. Once approved, samples should be prepared following the SOP of qPCR mRNA Preparation.
454 Junior Sequencing is currently restricted to UNC members that have completed training with the Vironomics Core.
- For those approved, please contact Core Manager for availability.
- Samples must meet below requirements or they are subject to rejection.
- NanoDrop is not compatible for assessing sequencing samples. If you do need to use the NanoDrop, please compensate for the overestimation and send 1ug.
- Amplicons: This technology is sensitive to primer-dimers, please contact Core Manager for Roche protocol of removing small fragments using AMPure beads.
*Please note that the Vironomics Core offers the PicoGreen reagents and fluorometer as part of the sequencing service. We also have an Agilent for assessing base-pair size.