398 Form Pages

BIOGRAPHICAL SKETCH[JG1] Provide the following information for the key personnel in the order listed for Form Page 2. Follow this format for each person. DO NOT EXCEED FOUR PAGES.

NAME Carol Aline Otey		POSITION TITLE Assistant Professor Dept. of Cell and Molecular Physiology
EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.)
INSTITUTION AND LOCATION	DEGREE (if applicable)		YEAR(s)	FIELD OF STUDY
Trinity University, San Antonio, TX University of California, Los Angeles, CA	B.S. Ph.D.		1980 1987	Biology Cell Biology

A. Positions and Honors.

Positions and Employment

1982-1985 NIH Predoctoral Trainee, University of California at Los Angeles, Dept. of Biology

1985-1987 Graduate Research Assistant, Laboratory of Dr. J.C. Bulinski, U.C.L.A., Dept. of Biology

1987-1992 NIH Postdoctoral Trainee, Lineberger Cancer Research Center, U.N.C. at Chapel Hill,

lab of Dr. Keith Burridge

1993-1998 Assistant Professor, Department of Cell Biology, University of Virginia, Charlottesville, VA

1998-present Assistant Professor, Department of Cell and Molecular Physiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina

Honors

1997 Dean’s Award for Excellence in Medical Education

B. Selected peer-reviewed publications (in chronological order), out of 33 total.

1. Otey-Rambo, C. and C.M. Szego. 1983. Estrogen action at endometrial membranes: Alternations in luminal surface detectable within seconds. J. Cell Biol. 97: 679-685.

2. Otey, C.A., M.H. Kalnoski, J.L. Lessard and J.C. Bulinski. 1986. Immunolocalization of the gamma isoform of non-muscle actin in cultured cells. J. Cell Biol. 102: 1726-1737.

3. Otey, C.A., M.H. Kalnoski and J.C. Bulinski. 1986. A procedure for the immunoblotting of proteins separated on isoelectric focusing gels. Anal. Biochem. 157: 71-76.

4. Otey, C.A., M.H. Kalnoski and J.C. Bulinski. 1987. Identification and quantification of actin isoforms in vertebrate cells and tissues. J. Cellular Biochem. 34: 113-124.

5. Otey, C.A., M.H. Kalnoski and J.C. Bulinski. 1988. Immunolocalization of muscle and non-muscle isoforms of actin in myogenic cells and adult skeletal muscle. Cell Motil. Cytoskel. 9: 337-348.

6. Pavalko, F.M., C.A. Otey and K. Burridge. 1989. Identification of a filamin isoform enriched at the ends of

stress fibers in chicken embryo fibroblasts. J. Cell Science 94: 109-118.

7. Otey, C.A., W. Griffiths and K. Burridge. 1990. Characterization of monoclonal antibodies to chicken gizzard talin. Hybridoma 9: 57-62.

8. Otey, C.A., F.M. Pavalko, and K. Burridge. 1990. An interaction between a-actinin and the b-1 integrin subunit in vitro. J. Cell Biol. 111: 721-729.

9. Bockholt, S.M., C.A. Otey, J.R. Glenney and K. Burridge. 1992. Localization of 215 kD tyrosine-phosphorylated protein that cross reacts with tensin antibodies. Exp. Cell Res. 203: 39-46.

10. Otey, C.A., G.B. Vasquez, K. Burridge and B.W. Erickson. 1993. Mapping of the a-actinin binding site within the ß₁ integrin cytoplasmic domain. J. Biol. Chem. 268: 21193-21197.

11. Eckert, W.A., J.G. Valtschanoff, C.A. Otey, A.L. Rustioni and R.J. Weinberg. 1994. Tyrosine phosphorylation in rat spinal cord after sciatic nerve transection. Neuroreport 5: 1289-1292.

12. Schaller, M.D., C.A. Otey, J.D. Hildebrand and J.T. Parsons. 1995. Focal adhesion kinase and paxillin bind to peptides mimicking b integrin cytoplasmic domains. J. Cell Biol., 130:1181-1188.

13. Hungerford, J., M.I. Compton, M.L. Matter, B.G. Hoffstrom and C.A. Otey. 1996. Inhibition of pp125^FAK in cultured fibroblasts results in apoptosis. J. Cell Biol. 135: 1383-1390.

14. Otey, C.A. 1998. A role for pp125FAK in suppression of apoptosis in fibroblasts. Biol Bull. 194(3):387-9.

15. Kiosses, W.B., R.H. Daniels, C. Otey, G.M. Bokoch and M.A. Schwartz. 1999. A role for PAK in endothelial cell migration. J. Cell Biol. 147: 831-843.

16. Parast, M.M. and C.A. Otey. 2000. Characterization of palladin, a novel protein localized to stress fibers and cell adhesions. J. Cell Biol. 150: 643-656.

17. Ren, X.-D., W.B. Kiosses, D.J. Sieg, C. Otey, D.D. Schlaepfer, and M.A. Schwartz. 2000. Focal adhesion kinase suppresses rho activity to promote focal adhesion turnover. J. Cell Sci.113: 3673-3678.

18. Edlund, N.M., M.A. Lotano and C.A. Otey. 2001. Dynamics of a-actinin in focal adhesions and stress fibers visualized with a-actinin Green Fluorescent Protein. Cell Motil. Cytoskel. 48: 190-200.

19. Edlund, M., T. Miyamoto, R. A. Sikes, R. Ogle, G.W. Laurie, M.C. Farach-Carson, C.A. Otey, H.E. Zhau, and L. W. K. Chung. 2001. Integrin expression and usage by prostate cancer cell lines on laminin substrata. Cell Growth and Differentiation 12: 99-107.

20. Hwang, S.J., Pagliardini S., Boukelifa M., Parast, M.M., Otey C.A., Rustioni A., Valtschanoff J.G. 2001. Palladin is expressed in excitatory terminals in the rat central nervous system. J. Comparative Neurol. 436: 211-224.

21. Boukhelifa, M., M.M. Parast, J.G. Valtschanoff, A.S. LaMantia, R.B. Meeker and C.A. Otey. 2001. A role for the cytoskeletal protein palladin in neurite outgrowth. Molec. Biol. Cell 12: 2721-2729.

22. Gonzalez, A.M., C. Otey, N.M. Edlund and J.C.R. Jones. 2001. Interactions of a hemidesmosome component and actinin family members. J. Cell Sci. 114: 4197-4206.

23. Vanni, S., B.C. Lagerholm, C.A. Otey, D. Velegol, D.L. Taylor and F. Lanni. 2001. Fibroblast mechanics in a model extracellular matrix – an application of optical sectional microscopy. Euro. Cells Materials 2: 21-22.

24. Rajfur, Z., P. Roy, C. Otey, L. Romer and K. Jacobson. 2002. The connection between stress fibers and focal adhesions: Dissecting the link employing chromophore assisted laser inactivation (CALI) with EGFP-fusion proteins. Nature Cell Biology 4: 286-293.

25. Tsuruta, D., M. Gonzales, S. B. Hopkinson, C. Otey, S. Khuon, R. D. Goldman, and J. C. R. Jones . 2002. Microfilament-dependent movement of the ß3 integrin subunit within focal contacts of endothelial cells. FASEB J. 16: 866-8.

26. Bhatt A, Kaverina I, Otey C, Huttenlocher A. 2002. Regulation of focal complex composition and disassembly by the calcium-dependent protease calpain. J Cell Sci. 115: 3415-25.

27. Boukhelifa, M., S.-J. Hwang, J.G. Valtschanoff, R. Meeker, A. Rustioni and C. Otey. (2003) A critical role for palladin in astrocyte morphology and response to injury. In press, Molec. Cell. Neuroscience.

28. Otey, C., M. Boukhelifa and P. Maness. (2003) B35 neuroblastoma cells: an easily transfected, cultured cell model of central nervous system neurons. Methods in Cell Biology 71:287-304.

29. Vanni, S., B.C. Lagerholm, C. Otey, D.L Taylor, and F. Lanni. (2003) Internet-based image analysis quantifies contractile behavior of individual fibroblasts inside model tissue. Biophysical J. 84: 2715-2727.

C. Research Support.

Completed Support

R29GM50974 5/01/94-4/30/99

“Regulation of Focal Adhesion Structure”,

NIH/NIGMS

Major goals: to characterize the interactions of both integrin subunits (a and b) with cytoplasmic binding partners such as a-actinin, talin and pp125FAK. A major finding from these studies was that inhibition of the binding interaction between b integrin and pp125FAK resulted in apoptotic cell death in cultured fibroblasts.

Role: P.I.

Ongoing Support

RO1 GM61743 4/1/02 – 3/30/06

“Biochemical Regulation of Actin Cytoskeleton Assembly”

NIH/NIGMS

Major goals: to identify binding partners for the cytoskeletal scaffold protein palladin in fibroblasts; to map their binding sites and perform a mutational analysis by overexpression in cultured cells; and to explore the role of palladin post-translational modification in cell rounding during mitosis.

Role: P.I.

R01 NS43253 9/30/02 - 7/31/07

“Role of Palladin in Regulating Astrocytes”

NIH/NINDS

Major goals: to determine if palladin expression directly controls shape change and actin assembly during astrocyte activation in response to injury, both in vitro and in vivo; to identify palladin’s binding partners in astrocytes.

Role: P.I.