There
are an enormous number of post-translational modification specific antibodies
that have been developed by both academic research labs and commercial sources
for the study of histone modifications.
Many chromatin labs have informally noted that these antibodies
cross-react with other histone PTMs.
Furthermore, little is known about how modifications neighboring the
intended epitope modulate binding. We have developed
an array-based technique for measuring the binding of histone PTM-specific
antibodies to greater than 100 peptides harboring one or more PTMs. As we collect data on antibodies we make or
purchase, we will report their binding specificities here. This is intended as a resource for researchers
but we will never be able to test all the available histone PTM-specific
antibodies (currently >1500).
However, if you would like information on a specific antibody and you
don’t see it below, please contact us and we can try to add it to our queue.
Histone
H3
|
Epitope |
Source |
|
|
|
|
H3R2me2assymetric |
Mark
Bedford (coming soon) |
|
|
|
|
H3T3 |
Active
Motif (39153 coming soon) |
|
|
|
|
H3K4me1 |
|
|
H3K4me1 |
|
|
|
|
|
H3K4me2 |
|
|
|
|
|
H3K4me3 |
|
|
H3K4me3 |
|
|
H3K4me3 |
|
|
|
|
|
H3K9me1 |
(coming
soon) |
|
H3K9me2 |
Millipore
17-648 (coming soon) |
|
H3K9me3 |
Millipore
17-625 (coming soon) |
|
|
|
|
H3K9acS10phos |
|
|
|
|
|
H3S10phos |
|
|
|
|
|
H3K14ac |
|
|
H3K14ac |
|
|
H3K14ac |
|
|
|
|
|
H3K36me1 |
Abcam ab1783 (coming soon) |
|
|
|
|
H3K36me2 |
Active
Motif (coming soon) |
|
|
|
|
H3K36me3 |
Abcam 9050
(coming soon) |
|
|
|
|
H3K79me1 |
|
|
H3K79me2 |
|
|
H3K79me3 |
Abcam ab2621 (lot 809870) (lot 441039) |
Histone
H4
|
Epitope |
Source |
|
|
|
|
H4 tetraacetyl |
|
|
|
|
|
H4K20me1 |
(coming
soon) |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
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|