HISTONEANTIBODIES

 

There are an enormous number of post-translational modification specific antibodies that have been developed by both academic research labs and commercial sources for the study of histone modifications.Many chromatin labs have informally noted that these antibodies cross-react with other histone PTMs.Furthermore, little is known about how modifications neighboring the intended epitope modulate binding. We have developed an array-based technique for measuring the binding of histone PTM-specific antibodies to greater than 100 peptides harboring one or more PTMs.As we collect data on antibodies we make or purchase, we will report their binding specificities here.This is intended as a resource for researchers but we will never be able to test all the available histone PTM-specific antibodies (currently >1500).However, if you would like information on a specific antibody and you donít see it below, please contact us and we can try to add it to our queue.

 

Histone H3

 

Epitope

Source

 

 

H3R2me2assymetric

Mark Bedford (coming soon)

 

 

H3T3

Active Motif (39153 coming soon)

 

 

H3K4me1

Upstate 07-436

H3K4me1

Millipore 07-436

 

 

H3K4me2

Active Motif 39142

 

 

H3K4me3

Active Motif 39160

H3K4me3

Millipore 07-473

H3K4me3

Abcam ab1012

 

 

H3K9me1

(coming soon)

H3K9me2

Millipore 17-648 (coming soon)

H3K9me3

Millipore 17-625 (coming soon)

 

 

H3K9acS10phos

Cell Signaling 9711

 

 

H3S10phos

Active Motif 39253

 

 

H3K14ac

Active Motif 39616

H3K14ac

Millipore 07-353

H3K14ac

Abcam ab46984

 

 

H3K36me1

Abcam ab1783 (coming soon)

 

 

H3K36me2

Active Motif (coming soon)

 

 

H3K36me3

Abcam 9050 (coming soon)

 

 

H3K79me1

Active Motif 39146

H3K79me2

Millipore 04-835

H3K79me3

Abcam ab2621 (lot 809870)†† (lot 441039)

 

 

 

Histone H4

 

Epitope

Source

 

 

H4 tetraacetyl

Active Motif 39179

 

 

H4K20me1

(coming soon)