Protocols adapted from Dong XR, Maguire CT, Wu SP, Majesky MW. Methods Enzymol. 2008;445:209-28:
Analysis of PE and epicardium in vivo by SEM
Explant culture of the epicardim
Isolation of total RNA from individual PEs for gene expression studies
Microdissection and explant culture of the proepicardium
Whole mount immunostaining methods for embryos
