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from Okada et.al. Nature 2007.Figure 3: Defective Chromatin condensation in Jhdm2a KO spermatids.
a , Haematoxylin and eosin staining shows the histopathological features of testis from seven-month-old Jhdm2a +/+ (+/+) and Jhdm2a G / G ( G / G ) mice. Roman numerals refer to the stages of mouse spermatogenesis. In Jhdm2a G / G mouse testis, spermatids with abnormal elongation were initially observed at stage XI (step 11). Insets represent higher-magnification images of elongated spermatids. b , Electron microscopy analysis of Jhdm2a +/+ (+/+) and Jhdm2a G / G ( G / G ) spermatids. Chromocentre at step 2–3 of Jhdm2a G / G spermatids is indistinct. In steps 7–9, heterochromatin distribution loses polarity. In step 13, chromatin condensation is defective. c , Abnormal morphology of spermatozoa isolated from cauda epididymis of Jhdm2a G / G ( G / G ) compared to spermatozoa from Jhdm2a +/+ (+/+). Jhdm2a G / G spermatozoa exhibit round shaped heads and variable size. d , Sperm motility analysis of the indicated genotypes. e , Acridine orange staining of spermatozoa from Jhdm2a +/+ (+/+) and Jhdm2a G / G ( G / G ) mice. Spermatozoa with complete chromatin condensation are only stained green, but are stained both green and red if their chromatin condensation is incomplete. Arrows indicate individual sperm. Blue staining represents Hoechst. |
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