{"id":2287,"date":"2019-07-16T14:02:12","date_gmt":"2019-07-16T18:02:12","guid":{"rendered":"https:\/\/www.med.unc.edu\/airwaybiocore\/?page_id=2287"},"modified":"2026-03-10T16:05:24","modified_gmt":"2026-03-10T20:05:24","slug":"research-applications","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/airwaybiocore\/research-applications\/","title":{"rendered":"Protocols and Research Applications"},"content":{"rendered":"

Core Protocols:
\n<\/strong><\/h4>\n

Cell Count<\/a>
\nCell Passaging Double Trypsinization<\/a>
\nCell Passaging with Accutase<\/a>
\nCell Plating Guidelines<\/a>
\nCoating Inserts- HPC-Collagen IV<\/a>
\nCoating Plastic Plates<\/a>
\nFreeze and Thaw Protocol<\/a>
\nMaintenance of HBE Cells on Plastic<\/a>
\nPrimary HBE Instructions<\/a>
\nWell-Differentiated Cell Cultures<\/a>
\nBMI Cell Line Instructions<\/a><\/p>\n

Papers Featuring Our Research:<\/strong><\/h4>\n

Well-Differentiated Human Airway Epithelial Cell Culture<\/a><\/p>\n

Novel Human Bronchial Epithelial Cell Lines for Cystic Fibrosis Research<\/a><\/p>\n

Primary Epithelial Cell Models for Cystic Fibrosis Research<\/a><\/p>\n

Pharmacological Rescue of Conditionally Reprogrammed CF HBE cells<\/a><\/p>\n

<\/h4>\n

 <\/p>\n

 <\/p>\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

Core Protocols: Cell Count Cell Passaging Double Trypsinization Cell Passaging with Accutase Cell Plating Guidelines Coating Inserts- HPC-Collagen IV Coating Plastic Plates Freeze and Thaw Protocol Maintenance of HBE Cells on Plastic Primary HBE Instructions Well-Differentiated Cell Cultures BMI Cell Line Instructions Papers Featuring Our Research: Well-Differentiated Human Airway Epithelial Cell Culture Novel Human Bronchial … Read more<\/a><\/p>\n","protected":false},"author":22214,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-2287","page","type-page","status-publish","hentry","odd"],"acf":[],"_links_to":[],"_links_to_target":[],"_links":{"self":[{"href":"https:\/\/www.med.unc.edu\/airwaybiocore\/wp-json\/wp\/v2\/pages\/2287","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.med.unc.edu\/airwaybiocore\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.med.unc.edu\/airwaybiocore\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/airwaybiocore\/wp-json\/wp\/v2\/users\/22214"}],"replies":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/airwaybiocore\/wp-json\/wp\/v2\/comments?post=2287"}],"version-history":[{"count":1,"href":"https:\/\/www.med.unc.edu\/airwaybiocore\/wp-json\/wp\/v2\/pages\/2287\/revisions"}],"predecessor-version":[{"id":3568,"href":"https:\/\/www.med.unc.edu\/airwaybiocore\/wp-json\/wp\/v2\/pages\/2287\/revisions\/3568"}],"wp:attachment":[{"href":"https:\/\/www.med.unc.edu\/airwaybiocore\/wp-json\/wp\/v2\/media?parent=2287"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}