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Seminar: Gabe Lander PhD (Scripps)
October 8 @ 11:00 am - 12:00 pm
GABE LANDER, PHD, Professor, Department of Integrative Structural and Computational Biology, Scripps Research Institute Host: Rob McGinty
“The highs and lows of cryo-EM and its use in studying molecular machines of mass destruction”
Abstract:Technical and methodological advances in single-particle cryo-electron microscopy (cryo-EM) have expanded the technique into a resolution regime that was previously only attainable by X-ray crystallography. We demonstrate that it is possible to obtain cryo-EM reconstructions of macromolecular complexes that are smaller than 100 kDa to better than 3 Å resolution using a 200 keV TEM. We also also use this technique to study AAA+ quality control proteases that regulate diverse aspects of cellular biology through specialized protein degradation. Through structure determination and biochemistry, we have established a structural framework to understand how different members of the AAA+ superfamily achieve specialized, diverse biological functions.