Sep 10, 2014
from 11:00 AM to 12:00 PM
|Contact Name||Amanda Chang|
Open to the public
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Joint UNC/NCSU Biomedical Engineering and UNC Biochemistry & Biophysics Seminar
"Cell Sorting and Directed Evolution in Microfluidic Systems"
Tom Soh, PhD
Ruth Garland Professor
Department of Mechanical Engineering and Materials
University of California, Santa Barbara
Bio: Dr. Soh received his B.S. with a double major in Mechanical Engineering and Materials Science with Distinction from Cornell University, and Ph.D. in Electrical Engineering from Stanford University. Prior to joining UCSB in 2003, Dr. Soh served as the technical manager of MEMS Device Research Group at Bell Laboratories and Agere Systems. His current research interests are in analytical biotechnology, especially in high throughput screening, directed evolution and integrated biosensors. He is Co-Director at the Center for Stem Cell Biology & Engineering and Associate Director of the California Nanosystems Institute (CNSI). He is the recipient of the MIT Technology Review’s "TR 100" Award (2002), ONR Young Investigator Award (2004), Beckman Young Investigator Award (2005), ALA Innovator Award (2009), NIH Director’s TR01 Award (2009), The Guggenheim Fellowship (2010), NIH Edward Nagy Award (2011), Garland Endowed Chair at UCSB (2011) and Alexander von Humboldt Fellowship (2012).
Abstract: Current techniques in high performance molecular and cellular separations are limited by the inherent coupling among three competing parameters: throughput, purity, and recovery of rare species. Our group utilizes unique advantages of microfluidics technology to decouple these competing parameters by precise and reproducible generation of separation forces that are not accessible in conventional, macroscopic systems. In this seminar, we will first discuss novel electrokinetic, magnetophoretic and acoustophoretic separation devices to purify rare target cells from complex mixtures. Second, we will discuss integrated biosensors that combine these separation devices with molecular probes in a disposable microfluidic chip. These chips are capable of processing clinical samples and provide molecular diagnostic information at the point-of-care. Finally, we will present our recent work in directed evolution using microfluidic devices. We will provide theoretical and experimental evidence for extremely fast generation of affinity reagents, and present innovative methods of evolving molecular machines that are capable of performing complex molecular functions such as binding induced switching and conformation change.
Host: Leslie Parise, PhD