{"id":13454,"date":"2020-04-25T08:19:05","date_gmt":"2020-04-25T12:19:05","guid":{"rendered":"https:\/\/www.med.unc.edu\/biochem\/?p=13454"},"modified":"2020-04-30T14:29:46","modified_gmt":"2020-04-30T18:29:46","slug":"macinfac-wins-ncbc-iig-grant","status":"publish","type":"post","link":"https:\/\/www.med.unc.edu\/biochem\/news\/macinfac-wins-ncbc-iig-grant\/","title":{"rendered":"MacInFac Wins NCBC IIG Grant"},"content":{"rendered":"<p style=\"margin: 0px 0px 1.563rem;color: #4f4f4f;font-family: 'Open Sans', Helvetica, Arial, sans-serif;font-size: 16px;font-style: normal;font-weight: 400;letter-spacing: normal;text-align: start;text-indent: 0px;text-transform: none;background-color: #ffffff\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-13455 size-full\" src=\"https:\/\/www.med.unc.edu\/biochem\/wp-content\/uploads\/sites\/795\/2020\/04\/macinfac-cropped-banner-photo.png\" alt=\"MacInFac green dots image\" width=\"993\" height=\"270\" srcset=\"https:\/\/www.med.unc.edu\/biochem\/wp-content\/uploads\/sites\/795\/2020\/04\/macinfac-cropped-banner-photo.png 993w, https:\/\/www.med.unc.edu\/biochem\/wp-content\/uploads\/sites\/795\/2020\/04\/macinfac-cropped-banner-photo-300x82.png 300w, https:\/\/www.med.unc.edu\/biochem\/wp-content\/uploads\/sites\/795\/2020\/04\/macinfac-cropped-banner-photo-768x209.png 768w, https:\/\/www.med.unc.edu\/biochem\/wp-content\/uploads\/sites\/795\/2020\/04\/macinfac-cropped-banner-photo-600x163.png 600w\" sizes=\"auto, (max-width: 993px) 100vw, 993px\" \/>The Macromolecular Interactions Facility (MacInFac) recently applied for and was just awarded, a North Carolina Biotechnology Center (NCBC) innovation impact grant for a Biacore 8K, a high-throughput Surface Plasmon Resonance (SPR) -based biosensor.\u00a0 With its ultra-low noise level, high sensitivity and high throughput capability, it will not only replace the current aged instrumentation in the facility, but will add new capabilities.<br \/>\nThis is the 4th NCBC grant to Dr. Ash Tripathy, MacInFac Director.<\/p>\n<p style=\"margin: 0px 0px 1.563rem;color: #4f4f4f;font-family: 'Open Sans', Helvetica, Arial, sans-serif;font-size: 16px;font-style: normal;font-weight: 400;letter-spacing: normal;text-align: start;text-indent: 0px;text-transform: none;background-color: #ffffff\">SPR technology is considered the \u201cgold standard\u201d for exploring macromolecular interactions with their cognate ligands in real time, especially with limited sample amounts.\u00a0 Using a minute quantity of a macromolecule (5 \u00b5g), it can measure the on rate (kon), the off rate (koff), and the dissociation constant (KD) of a bi-molecular interaction.<\/p>\n<p style=\"margin: 0px 0px 1.563rem;color: #4f4f4f;font-family: 'Open Sans', Helvetica, Arial, sans-serif;font-size: 16px;font-style: normal;font-weight: 400;letter-spacing: normal;text-align: start;text-indent: 0px;text-transform: none;background-color: #ffffff\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-4913 alignright\" src=\"https:\/\/www.med.unc.edu\/biochem\/wp-content\/uploads\/sites\/795\/2018\/07\/tripathy_CROPPED.jpg\" alt=\"Dr. Ash Tripathy\" width=\"92\" height=\"128\" \/>Dr. Tripathy states: \u201cIn our facility, SPR technology has been used to explore antigen-antibody, DNA-protein, RNA-protein, protein-protein, protein-peptide, protein-small molecule, and RNA-small molecule interactions very successfully by many PI\u2019s in our campus and Triangle-based biotechnology companies. The Biacore 8K will not only be able to perform all these types of experiments, but its ultra-low noise level will allow us to explore binding kinetics of small molecule and fragment \u2013 with almost no lower MW detection cutoff \u2013 \u00a0drug \u201cleads\u201c \u00a0against protein and RNA targets, and the very tight binding kinetics of many designed antibodies against their respective ligands. In addition, it will be used as a platform for screening of small molecule and fragment libraries in drug discovery research.<\/p>\n<p style=\"margin: 0px 0px 1.563rem;color: #4f4f4f;font-family: 'Open Sans', Helvetica, Arial, sans-serif;font-size: 16px;font-style: normal;font-weight: 400;letter-spacing: normal;text-align: start;text-indent: 0px;text-transform: none;background-color: #ffffff\">In addition to its utilization for research purposes, the SPR-based biosensor is used for instruction in a graduate course \u201cMacromolecular Interactions\u201d (BIOC 662), which is a critical component of the Biophysics training program at UNC.<\/p>\n<p style=\"margin: 0px 0px 1.563rem;color: #4f4f4f;font-family: 'Open Sans', Helvetica, Arial, sans-serif;font-size: 16px;font-style: normal;font-weight: 400;letter-spacing: normal;text-align: start;text-indent: 0px;text-transform: none;background-color: #ffffff\">The new instrument is expected to be operational by early summer 2020.<\/p>\n<p style=\"margin: 0px 0px 1.563rem;color: #4f4f4f;font-family: 'Open Sans', Helvetica, Arial, sans-serif;font-size: 16px;font-style: normal;font-weight: 400;letter-spacing: normal;text-align: start;text-indent: 0px;text-transform: none;background-color: #ffffff\">The Macromolecular Interactions Facility, located in the Genome Sciences Building Room 1124,\u00a0 provides access to, and training on, an array of instrumentation for biophysical characterization of biological macromolecules and their interactions with cognate ligands.<\/p>\n<p style=\"margin: 0px 0px 1.563rem;color: #4f4f4f;font-family: 'Open Sans', Helvetica, Arial, sans-serif;font-size: 16px;font-style: normal;font-weight: 400;letter-spacing: normal;text-align: start;text-indent: 0px;text-transform: none;background-color: #ffffff\">Visit their website for more information:\u00a0<a href=\"https:\/\/www.med.unc.edu\/csb\/macinfac\/\" target=\"_blank\" rel=\"noopener noreferrer\">https:\/\/www.med.unc.edu\/csb\/macinfac\/<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>The Macromolecular Interactions Facility (MacInFac) recently applied for and was just awarded, a North Carolina Biotechnology Center (NCBC) innovation impact grant for a Biacore 8K, a high-throughput Surface Plasmon Resonance (SPR) -based biosensor.  <\/p>\n","protected":false},"author":41619,"featured_media":13455,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"layout":"","cellInformation":"","apiCallInformation":"","footnotes":"","_links_to":"","_links_to_target":""},"categories":[2],"tags":[330,78,358,263,10,298],"class_list":["post-13454","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-news","tag-2020-faculty-year-in-review","tag-biochemistry-and-biophysics","tag-core-news","tag-department-news","tag-news_faculty","tag-news_2020","odd"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>MacInFac Wins NCBC IIG Grant | Biochemistry and Biophysics<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" 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