{"id":18015,"date":"2021-05-02T11:51:39","date_gmt":"2021-05-02T15:51:39","guid":{"rendered":"https:\/\/www.med.unc.edu\/biochem\/?p=18015"},"modified":"2021-05-18T11:56:01","modified_gmt":"2021-05-18T15:56:01","slug":"2021-new-mass-photometer-in-unc-pep-mx-core","status":"publish","type":"post","link":"https:\/\/www.med.unc.edu\/biochem\/news\/2021-new-mass-photometer-in-unc-pep-mx-core\/","title":{"rendered":"2021: NEW Mass Photometer in UNC PEP-MX Core"},"content":{"rendered":"<p>The\u00a0<strong>UNC PEP-MX core<\/strong>\u00a0has a Refeyn One\u00a0<strong>Mass Photometry<\/strong>\u00a0instrument that\u2019s now available for use. The Mass Photometer measures minute perturbations in the refractive index of a solution as biomolecules tumble within. Sophisticated software then calculates the masses of the particles that produced those optical differences (Figure 1).<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-3955\" src=\"https:\/\/www.med.unc.edu\/corefacilities\/wp-content\/uploads\/sites\/943\/2021\/04\/UNC-PEP-MX-CORE.png\" alt=\"UNC PEP MX CORE\" width=\"1004\" height=\"751\" \/><\/p>\n<p>Figure 1. Recent experimental data acquired by the mass photometer on a joint project between the laboratories of Dr N Brown and Dr J Schisler (UNC Pharmacology). The sample was composed of the ubiquitin ligase known as CHIP (C-terminus of Hsc70-interacting protein). CHIP is known to exhibit successive oligomeric states, clearly identifiable in the histogram.<\/p>\n<p>&nbsp;<\/p>\n<p>The mass photometer has a range of 40 kDa \u2013 5 MDa with an accuracy ~5-10% of known. Sample needs are roughly 100 ul at 100 nM (~5 ug\/ml) or less, and the instrument can accommodate proteins, DNAs, RNAs, and virtually any assemblies thereof. The instrument can assess sample quality, oligomeric states, complex formation, and more. Contact the core (<a href=\"mailto:pepcore@med.unc.edu\">pepcore@med.unc.edu<\/a>) to schedule training and instrument time!<\/p>\n","protected":false},"excerpt":{"rendered":"<p>The\u00a0UNC PEP-MX core\u00a0has a Refeyn One\u00a0Mass Photometry\u00a0instrument that\u2019s now available for use. The Mass Photometer measures minute perturbations in the refractive index of a solution as biomolecules tumble within. Sophisticated software then calculates the masses of the particles that produced those optical differences (Figure 1). Figure 1. Recent experimental data acquired by the mass photometer &hellip; <a href=\"https:\/\/www.med.unc.edu\/biochem\/news\/2021-new-mass-photometer-in-unc-pep-mx-core\/\" aria-label=\"Read more about 2021: NEW Mass Photometer in UNC PEP-MX Core\">Read more<\/a><\/p>\n","protected":false},"author":41619,"featured_media":18016,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"layout":"","cellInformation":"","apiCallInformation":"","footnotes":"","_links_to":"","_links_to_target":""},"categories":[2],"tags":[10,363,179],"class_list":["post-18015","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-news","tag-news_faculty","tag-news_2021","tag-news_postdoc-scholars","odd"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>2021: NEW Mass Photometer in UNC PEP-MX Core | Biochemistry and Biophysics<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.med.unc.edu\/biochem\/news\/2021-new-mass-photometer-in-unc-pep-mx-core\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"2021: NEW Mass Photometer in UNC PEP-MX Core | Biochemistry and Biophysics\" \/>\n<meta property=\"og:description\" content=\"The\u00a0UNC PEP-MX core\u00a0has a Refeyn One\u00a0Mass Photometry\u00a0instrument that\u2019s now available for use. The Mass Photometer measures minute perturbations in the refractive index of a solution as biomolecules tumble within. Sophisticated software then calculates the masses of the particles that produced those optical differences (Figure 1). Figure 1. Recent experimental data acquired by the mass photometer &hellip; Read more\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.med.unc.edu\/biochem\/news\/2021-new-mass-photometer-in-unc-pep-mx-core\/\" \/>\n<meta property=\"og:site_name\" content=\"Biochemistry and Biophysics\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/uncbiochemistryandbiophysics\/\" \/>\n<meta property=\"article:published_time\" content=\"2021-05-02T15:51:39+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2021-05-18T15:56:01+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.med.unc.edu\/biochem\/wp-content\/uploads\/sites\/795\/2021\/05\/unc-som-core-facilities.png\" \/>\n\t<meta property=\"og:image:width\" content=\"425\" \/>\n\t<meta property=\"og:image:height\" content=\"161\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/png\" \/>\n<meta name=\"author\" content=\"Carolyn Clabo\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@UNC_BCBP\" \/>\n<meta name=\"twitter:site\" content=\"@UNC_BCBP\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Carolyn Clabo\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\/\/www.med.unc.edu\/biochem\/news\/2021-new-mass-photometer-in-unc-pep-mx-core\/#article\",\"isPartOf\":{\"@id\":\"https:\/\/www.med.unc.edu\/biochem\/news\/2021-new-mass-photometer-in-unc-pep-mx-core\/\"},\"author\":{\"name\":\"Carolyn Clabo\",\"@id\":\"https:\/\/www.med.unc.edu\/biochem\/#\/schema\/person\/9693a4e0a76e8208ca2105ae25587332\"},\"headline\":\"2021: NEW Mass Photometer in UNC PEP-MX Core\",\"datePublished\":\"2021-05-02T15:51:39+00:00\",\"dateModified\":\"2021-05-18T15:56:01+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\/\/www.med.unc.edu\/biochem\/news\/2021-new-mass-photometer-in-unc-pep-mx-core\/\"},\"wordCount\":176,\"publisher\":{\"@id\":\"https:\/\/www.med.unc.edu\/biochem\/#organization\"},\"image\":{\"@id\":\"https:\/\/www.med.unc.edu\/biochem\/news\/2021-new-mass-photometer-in-unc-pep-mx-core\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/www.med.unc.edu\/biochem\/wp-content\/uploads\/sites\/795\/2021\/05\/unc-som-core-facilities.png\",\"keywords\":[\"Faculty &amp; 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