{"id":3925,"date":"2016-05-19T17:20:00","date_gmt":"2016-05-19T21:20:00","guid":{"rendered":"https:\/\/www.med.unc.edu\/bric\/high-resolution-contrast-enhanced-ct-on-froglet\/"},"modified":"2018-08-23T18:08:53","modified_gmt":"2018-08-23T22:08:53","slug":"high-resolution-contrast-enhanced-ct-on-froglet","status":"publish","type":"post","link":"https:\/\/www.med.unc.edu\/bric\/high-resolution-contrast-enhanced-ct-on-froglet\/","title":{"rendered":"High resolution contrast enhanced CT on froglet"},"content":{"rendered":"<div>\n<p class=\"lead\">High resolution CT imaging is used to delineate cardiac structure of Xenopus froglets in Frank Conlon\u2019s group.<\/p>\n<div class=\"image-section\">\n<figure class=\"thumbnail wp-caption alignright\">\n    <img loading=\"lazy\" decoding=\"async\" class=\"size-medium wp-image-3926\" src=\"https:\/\/www.med.unc.edu\/bric\/wp-content\/uploads\/sites\/843\/2018\/08\/high-resolution-contrast-enhanced-ct-on-froglet-image2-300x300.png\" width=\"300\" height=\"200\" alt=\"image2\" srcset=\"https:\/\/www.med.unc.edu\/bric\/wp-content\/uploads\/sites\/843\/2018\/08\/high-resolution-contrast-enhanced-ct-on-froglet-image2-768x512.png 768w, https:\/\/www.med.unc.edu\/bric\/wp-content\/uploads\/sites\/843\/2018\/08\/high-resolution-contrast-enhanced-ct-on-froglet-image2-600x400.png 600w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><figcaption class=\"caption wp-caption-text\">Micro-CT image taken from Xenopus froglet at 10um resolution and analyzed with VivoQuant software.  Upper left hand corner is 3D rendered heart valves segmented from the CT images.<br \/>\n    <\/figcaption><\/figure>\n<figure class=\"thumbnail wp-caption alignright\">\n    <img loading=\"lazy\" decoding=\"async\" class=\"size-medium wp-image-3927\" src=\"https:\/\/www.med.unc.edu\/bric\/wp-content\/uploads\/sites\/843\/2018\/08\/high-resolution-contrast-enhanced-ct-on-froglet-image3-300x297.png\" width=\"300\" height=\"200\" alt=\"image3\"\/><figcaption class=\"caption wp-caption-text\">Closeup of CT slice of Xenopus heart taken at 10um resolution following staining with the CT contrast agent.<br \/>\n    <\/figcaption><\/figure>\n<\/div>\n<div>\n<p>We have recently developed a high resolution contrast enhanced CT imaging protocol for a study on Xenopus froglets.  Dr. Frank Conlon\u2019s group has  been using  Xenopus froglets as an animal model to study the genetic causes of congenital heart disease. The focus of the study is to ascertain how the Xenopus heart develops and grows and to help determine its structural similarity to the mammalian heart. Using high resolution microCT, detailed structures and defects in froglet heart can be delineated without standard histological methods on the heart tissue.  The froglet\u2019s  were fixed at NF stage 66 and had a torso length of approximately 1cm, which is a newly metamorphosed froglet.  The images below were obtained with the SCANCO uCT40 with a voxel size of 10um following PTA contrast staining.  Post-processing was completed with VivoQuant software.  The image below shows a 3D rendering of the froglet heart with the segmented valves shown in light pink.<\/p>\n<p><img decoding=\"async\" src=\"https:\/\/www.med.unc.edu\/bric\/wp-content\/uploads\/sites\/843\/2018\/08\/froglet-render.gif\" alt=\"Froglet render\" class=\"image-inline\" title=\"Froglet render\"\/> <\/p>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<\/p>\n<p>High resolution CT imaging is used to delineate cardiac structure of Xenopus froglets in Frank Conlon\u2019s group.<\/p>\n","protected":false},"author":41912,"featured_media":3926,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"categories":[2],"tags":[],"class_list":["post-3925","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-news","odd"],"pp_force_visibility":null,"pp_subpost_visibility":null,"pp_inherited_force_visibility":null,"pp_inherited_subpost_visibility":null,"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.8 - 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