Philpot - Images

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Figure 1. Visual experience in wild-type (wt) mice alters the properties of synaptic plasticity. A) Low-magnification view of a visual cortex slice preparation. Field potentials are recorded with a glass micropipette in layers 3 and evoked by layer 4 stimulation. B) Changes in synaptic strength can by measured by the amplitude of the field potential before and after high-frequency stimulation. This figure demonstrates that 40 Hz stimulation increases synaptic strength more in visual cortex of dark-reared (DR) mice than light-reared controls (LR).

 

 

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Figure 2. Visual experience alters NMDA receptor currents. A) Example of a whole-cell recording made from a visualized layer 3 pyramidal cell. B) NMDA receptor currents are longer in cells from dark-reared cortex as compared to light-reared controls.

 

 

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Figure 3. Immunoblot demonstrating protein levels of NR2A, NR1, GluR1, and synaptophysin (Syn) in wild-type (+/+), heterozygote (+/-), and NR2A knockout (-/-) mice. The NR2A NMDA receptor subunit is absent in NR2A knockout mice, while NR1 NMDA receptor subunit and the GluR1 AMPA receptor subunit levels remain unchanged. Levels of the synaptic protein, synaptophysin, are used for normalization purposes.

 

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Figure4. A layer 3 pyramidal cell filled with a fluorescent marker.