The Molecular Biology Core

Director: Wanda K O'Neal, PhD, Research Associate Professor


The Molecular Biology Core was established to provide expertise, equipment, training, and reagents to serve the molecular biology needs for Cystic Fibrosis Research.


  • The Core serves as a centralized resource for cloning and sequencing of genes.
  • Isolation, storage, and analysis of high quality RNA.
  • Generation of transgenic mouse models.
  • Development of methods for si/shRNA knockdown in airway cell models.
  • Immunohistochemistry.
  • Providing training in molecular biology.

Molecular biology has become an indispensable component of modern biological research programs. In order to continue to move the CF Center at UNC forward and to keep its competitive edge, the core plans to become proficient at RNA and miRNA sequencing, utilizing next generation sequencing technologies to advance CF research.


 Update: New Equipment in the Core: As part of the Molecular Therapy Core Center AARA equipment funding, the Molecular Biology Cor has been able to purchase a new imaging system! Please look over the FluorChemQ brochure from  Alpha Innotech for detailed information. It is designed for quantitative Western blotting with chemiluminescence and fluorescence imaging, but it can be used for a variety of applications, including fluorescent DNA and protein gels (ethidium bromide stains, etc.), colorimetric protein gels, and colony plate imaging. In addition, it can accept multi-plex fluorescent westerns because it has a variety of emissions filters built in, so it can quantify more than one protein per gel and resolve co-migrating proteins. It also has image capture software for ease of publication, as well as record keeping and software for accurate quantification.

Molecular Core Personnel

Group with Dr. Wanda O'Neal, Director

Lisa Jones, Research Specialist

Rodney C. Gilmore, Research Specialist









Kristy Terrell, Research Specialist








Contact Information

7027 Thurston-Bowles Building
Campus Box #7248
The University of North Carolina at Chapel Hill
Chapel Hill, NC 27599
(919) 962 9866
Fax:(919) 966 6821