The CF Center Molecular Biology Core

Mission Services Equipment Personnel Contact

Director: Wanda K O'Neal, PhD, Research Associate Professor

Our Mission

The Molecular Biology Core was established to provide expertise, equipment, training, and reagents to serve the molecular biology needs for Cystic Fibrosis Research.

Services Available in the Molecular Core

  • The Core serves as a centralized resource for cloning and sequencing of genes.
  • Isolation, storage, and analysis of high quality RNA.
  • Generation of transgenic mouse models.
  • Development of methods for si/shRNA knockdown in airway cell models.
  • Immunohistochemistry.
  • Providing training in molecular biology.

Molecular biology has become an indispensable component of modern biological research programs. In order to continue to push the CF Center at UNC forward and to keep us competitive we are planning on becoming proficient at RNA and miRNA sequencing, utilizing next generation sequencing technologies to advance CF research.

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Equipment Available in the Molecular Core

The Core's newest instrument: an Alpha Innotech FluroChem Imager

Hood dedicated solely for RNA isolation

Our PCR room has both a Roche 480 Lightcycler and an ABI 7500 Fast Instrument

 

 

 

 

 

 

 

 

 

 

 


Update: New Equipment in the Core: As part of the Molecular Therapy Core Center AARA equipment funding, the Molecular Biology Cor has been able to purchase a new imaging system! Please look over the FluorChemQ brochure from  Alpha Innotech for detailed information. It is designed for quantitative Western blotting with chemiluminescence and fluorescence imaging, but it can be used for a variety of applications, including fluorescent DNA and protein gels (ethidium bromide stains, etc.), colorimetric protein gels, and colony plate imaging. In addition, it can accept multi-plex fluorescent westerns because it has a variety of emissions filters built in, so it can quantify more than one protein per gel and resolve co-migrating proteins. It also has image capture software for ease of publication as well as record keeping and software for accurate quantification.

Molecular Core Personnel

Group with Dr. Wanda O'Neal, Director

Lisa Jones, Research Specialist

Rodney C. Gilmore, Research Specialist

 

 

 

 

 

 

 

 

 

Kristy Terrell, Research Specialist

 

 

 

 

 

 

 

 

 

 

Contact Information

7027 Thurston-Bowles Building
Campus Box #7248
The University of North Carolina at Chapel Hill
Chapel Hill, NC 27599
(919) 962 9866
Fax:(919) 966 6821
E-mail: woneal@email.unc.edu