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Epithelial Organoids and 2D Monolayer “EpCulture” Services Offered by the Advanced Analytics Core
Organoid technologies have transformed in vitro GI tract research, but they pose unique challenges due to their non-traditional culture methods. Organoids are grown in 3D matrigel patties that require more difficult culture techniques than standard 2D methods, and their spherical structures are challenging to evaluate by microscopy. To address these challenges, investigators at UNC have pioneered 2D monolayer culture methods that use the same initial starting material as 3D organoid cultures (typically epithelium in the form of small intestinal or colonic crypts). 2D monolayers are more convenient to work with since they can be passaged and cryopreserved using standard methods. The UNC Advanced Analytics Core now offers specialized conditioned media to facilitate both 2D and 3D organoid culture. Conditioned medias are available to UNC researchers only.

Conditioned Media Products and Pricing
Note: For most purposes, conditioned media is supplied at 2X– this makes the effective price only $1/mL!

*I: Intestinal, C: Colonic, G: Gastric, P: Pancreatic, L: Liver, B: Billiary

Submitting Orders:

Please email Quinn Fong ( regarding any service requests or general inquiries

AA Core MBRB Laboratory

Medical Biomolecular Research Building (MBRB)

Room 4331

111 Mason Farm Rd

Chapel Hill, NC 27599