Services:
  • Sample preparation via FACS
  • RNA-Seq (up to 800 cells)
  • REAP-Seq (up to 800 cells)
  • DNA-Seq (up to 96 cells)
  • qPCR (up to 96 cells)
  • Sequencing and basic data processing

The Advanced Analytics Core offers turnkey Fluidigm C1 single cell services. Simply provide a single cell suspension, and the Core will perform the services necessary to generate high quality sequencing data: cell sorting, library preparation, sequencing, and basic data analysis.

The C1 system isolates single cells into individual reaction chambers in the exclusive Fluidigm integrated fluidic circuit (IFC). The optically clear IFC lets you automatically stain captured cells and examine them by microscopy for viability, surface markers, or reporter genes. After staining, cells are automatically lysed and template is quickly prepared for sequencing or qPCR—all in as little as four hours. Applications include:

  • Studying cell differentiation
  • Measuring individual cell responses to specific stimuli
  • Verifying critical disease biomarkers
  • Validating RNAi knockdown
  • Conducting candidate drug screens
  • Custom-designed applications using Fluidigm’s Script Hub

Instruments:
How to start a project:

To begin planning an immunoassay project with the AA Core, please contact the Core Manager to arrange a consultation.


Pricing estimates:
Service Price* UNC/NCSU/CGIBD Member Price**
RNA-Seq (per IFC) ~$4,980 $3,200
HT RNA-Seq (per IFC) ~$5,450 $3,500
Pre-amp for qPCR (per IFC) ~$2,340 $1,500
REAP-Seq (per IFC) Pricing available upon request
DNA-Seq (per IFC)

* Price estimate for standard service. A customized quote can be provided upon consultation.

** Investigators affiliated with UNC, NCSU, or the CGIBD receive a ~35% discount

Sequencing & Basic Data Processing
Service Price* UNC/NCSU/CGIBD Member Price**
NextSeq Mid-Output 150 cycles ~$2,220 ~$1,425
NextSeq High-Output 150 cycles ~$5,640 ~$3,625

* Price estimate for standard service including materials, labor, and basic data processing.

** Investigators affiliated with UNC, NCSU, or the CGIBD receive a ~35% discount


Submitting Samples:

Users may schedule an appointment to drop off samples in the AA Core single cell laboratory:

AA Core MBRB Laboratory

Medical Biomolecular Research Building (MBRB)

Room 4331

111 Mason Farm Rd

Chapel Hill, NC 27599