Single Cell Genomics (Fluidigm/Standard BioTools C1)
Fluidigm C1 Services:
- Sample preparation via FACS
- RNA-Seq (up to 800 cells)
- REAP-Seq (up to 800 cells)
- DNA-Seq (up to 96 cells)
- qPCR (up to 96 cells)
- Sequencing and basic data processing
The Advanced Analytics Core offers turnkey Fluidigm C1 single cell services. Simply provide a single cell suspension, and the Core will perform the services necessary to generate high quality sequencing data: cell sorting, library preparation, sequencing, and basic data analysis.
The C1 system isolates single cells into individual reaction chambers in the exclusive Fluidigm integrated fluidic circuit (IFC). The optically clear IFC lets you automatically stain captured cells and examine them by microscopy for viability, surface markers, or reporter genes. After staining, cells are automatically lysed and template is quickly prepared for sequencing or qPCR—all in as little as four hours. Applications include:
- Studying cell differentiation
- Measuring individual cell responses to specific stimuli
- Verifying critical disease biomarkers
- Validating RNAi knockdown
- Conducting candidate drug screens
- Custom-designed applications using Fluidigm’s Script Hub
Instruments:
How to start a project:
To begin planning an immunoassay project with the AA Core, please contact the Associate Director to arrange a consultation.
Pricing estimates:
| Service | Price* | UNC/NCSU/CGIBD Member Price** |
| RNA-Seq (per IFC) | ~$5,040 | $3,240 |
| HT RNA-Seq (per IFC) | ~$5,575 | $3,580 |
| Pre-amp for qPCR (per IFC) | ~$2,395 | $1,540 |
| REAP-Seq (per IFC) | Pricing available upon request | |
| DNA-Seq (per IFC) | ||
* Price estimate for standard service. A customized quote can be provided upon consultation.
** Investigators affiliated with UNC, NCSU, or the CGIBD receive a ~35% discount
Sequencing & Basic Data Processing
| Service | Price* | UNC/NCSU/CGIBD Member Price** |
| NextSeq Mid-Output 150 cycles | ~$2,040 | ~$1,310 |
| NextSeq High-Output 150 cycles | ~$5,150 | ~$3,310 |
* Price estimate for standard service including materials, labor, and basic data processing.
** Investigators affiliated with UNC, NCSU, or the CGIBD receive a ~35% discount
Submitting Samples:
Users may schedule an appointment to drop off samples in the AA Core single cell laboratory:
AA Core MBRB Laboratory
Medical Biomolecular Research Building (MBRB)
Room 4331
111 Mason Farm Rd
Chapel Hill, NC 27599