Immunotechnologies (ELISA & Multiplex)

Immunotechnology Service:

• Consultation
• ELISA/EIA and multiplex assays
• Plate reading & data analysis

The Advanced Analytics (AA) Core’s immunotechnologies component provides consultation, ordering, and assay service for most commercially available kits in both traditional ELISA/EIA and bead-based multiplex formats. The AA Core typically receives discounts of 20% or more from preferred vendors and the Core’s innovative cost-sharing system allows users to purchase immunoassay reagents they need in more cost-effective quantities and receive credits toward future assays when other users have need of them. When combined with the already lower cost of bulk reagents, paying to have your immunoassay work done by the AA Core can save a user hundreds or even thousands of dollars a year.

Instruments:

• Bio-Rad Bio-Plex 200 multiplex suspension array system with high-throughput fluidics
• Curiox LT210 bead plate washing system for Luminex and Singulex SMC assays
• BioTek Instruments Synergy HT microplate spectrophotometer
• BioTek Instruments 405LSRS microplate washer

Services:

Consultation: All prospective Core users receive up to one hour of complimentary consultation, including any assay research or purchasing advice, at no charge. Additional assistance with experimental planning or troubleshooting is available for a small fee.

ELISA/EIA & Multiplex Assay Services: Ordering and immunoassay services are available through the AA Core at very reasonable rates. Just schedule an assay, drop off your samples, and in a week or less you’ll have data.

Plate Reading & Data Analysis: Your assay plate will be read and you will be sent an electronic copy of your results, including some basic statistics (mean, standard deviation, % CV) and information about the standard curve (graph, formula, curve fitting results). Users who prefer to prep and run their own assays may purchase this as a stand-alone service.

How to start a project:

To begin planning an immunoassay project with the AA Core, please contact the Associate Director to arrange a consultation.

Pricing estimates:

* Price estimate for standard service. A customized quote can be provided upon consultation.

** Investigators affiliated with UNC, NCSU, or the CGIBD receive a ~35% discount

*** Only for samples not submitted in ready-to-assay form (i.e. samples which require plating, centrifugation, etc…)

Submitting Samples:

All samples should be submitted in ready-to-assay form on a minimum-binding 96-well plate (we recommend Fisher part number 12-565-505, available from the Core). Tubed samples will be charged $2 per sample for additional processing and handling. Please provide sufficient volume for all samples to be assayed in duplicate, plus an additional 25 uL. Samples to be diluted may instead be submitted at the volume required to dilute them up to 250-300 uL evenly (e.g., 25uL for a 1:10 dilution). Samples to be miniaturized via DropArray require less volume (75-100 uL).

Samples may be dropped off in Room 030 of the Glaxo Building. New users are advised to make an appointment to ensure that someone will be available to receive their samples.

Any pre-extraction or sample preparation procedures are the sole responsibility of the user.

AA Core Glaxo Laboratory

Glaxo Building

Room 030

101 Mason Farm Rd

Chapel Hill, NC 27599