{"id":6481,"date":"2021-02-19T12:47:03","date_gmt":"2021-02-19T17:47:03","guid":{"rendered":"https:\/\/www.med.unc.edu\/cgibd\/?post_type=directory&#038;p=6481"},"modified":"2024-03-05T16:12:38","modified_gmt":"2024-03-05T21:12:38","slug":"nathan-crook-phd","status":"publish","type":"directory","link":"https:\/\/www.med.unc.edu\/cgibd\/directory\/nathan-crook-phd\/","title":{"rendered":"Nathan Crook, PhD"},"content":{"rendered":"<h3><strong>Research Summary<\/strong><\/h3>\n<p>Dr. Crook\u2019s lab characterizes and enhances the ability of probiotic yeast (Saccharomyces boulardii) to synthesize therapeutics in the large intestine. The lab has developed the largest toolkit for engineering this strain and has characterized its colonization kinetics in a variety of mouse models. They have recently applied this knowledge to enable S. boulardii to produce the vitamin A precursor \u03b2-carotene in the colon, have enhanced its ability to secrete peptides by more than 10-fold via knockout of key proteases, and are currently investigating the ability of S. boulardii to produce peptides that inactivate the toxins of Clostridioides difficile. He has developed methods accelerating high-throughput screening in yeast, including the invention of retrotransposon-mediated continuous directed evolution, and a set of optimized procedures for genome-wide RNA interference screens. He also developed computer design algorithms that enable fine-tuned control of transcription and translation rates for the first time in yeast. The ultimate goal is to develop platform technologies that de-bottleneck genetic engineering and applying them to transform drug delivery.<\/p>\n<p><strong>CGIBD Focus Area(s):<\/strong>\u00a0 Microbiome<\/p>\n<p><strong>Collaborators:\u00a0\u00a0<\/strong>Magness<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Research Summary Dr. Crook\u2019s lab characterizes and enhances the ability of probiotic yeast (Saccharomyces boulardii) to synthesize therapeutics in the large intestine. The lab has developed the largest toolkit for engineering this strain and has characterized its colonization kinetics in a variety of mouse models. They have recently applied this knowledge to enable S. boulardii &hellip; <a href=\"https:\/\/www.med.unc.edu\/cgibd\/directory\/nathan-crook-phd\/\" aria-label=\"Read more about Nathan Crook, PhD\">Read more<\/a><\/p>\n","protected":false},"featured_media":5488,"template":"","meta":{"_acf_changed":false,"_links_to":"","_links_to_target":""},"class_list":["post-6481","directory","type-directory","status-publish","has-post-thumbnail","hentry","odd"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Nathan Crook, PhD | Center for Gastrointestinal Biology and Disease<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.med.unc.edu\/cgibd\/directory\/nathan-crook-phd\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Nathan Crook, PhD | Center for Gastrointestinal Biology and Disease\" \/>\n<meta property=\"og:description\" content=\"Research Summary Dr. Crook\u2019s lab characterizes and enhances the ability of probiotic yeast (Saccharomyces boulardii) to synthesize therapeutics in the large intestine. 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