{"id":5487,"date":"2020-01-09T12:48:09","date_gmt":"2020-01-09T17:48:09","guid":{"rendered":"https:\/\/www.med.unc.edu\/cgibd\/?page_id=5487"},"modified":"2020-01-09T12:59:19","modified_gmt":"2020-01-09T17:59:19","slug":"crook-nathan","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/cgibd\/crook-nathan\/","title":{"rendered":"Nathan Crook, PhD"},"content":{"rendered":"

<\/p>\n

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\"\"<\/p>\n
\n

Contact Information<\/h4>\n

North Carolina State University
\n911 Partners Way
\nBox 7905
\n(O) 919-513-2429<\/p>\n

nccrook@ncsu.edu<\/a><\/p>\n

Website Links<\/h4>\n

PubMed<\/a><\/p>\n

Lab Website<\/a><\/p>\n

<\/h4>\n<\/div>\n<\/th>\n

\n
Assistant Professor
\nDept. of Chemical and Biomedical Engineering
\nCollege of Engineering
\nNorth Carolina State University<\/span><\/strong><\/span><\/span><\/div>\n

Research Summary<\/strong><\/p>\n

Dr. Crook\u2019s research focuses on developing new methods for engineering microbes, and applying these methods to improve human health.\u00a0 As a graduate student in the laboratory of Dr. Hal Alper at UT Austin, Dr. Crook developed several new methods to genetically engineer baker\u2019s yeast, including RNAi screens and continuous directed evolution approaches using retrotransposons.\u00a0 Dr. Crook also developed model-based approaches for yeast engineering, encompassing the strengthening of yeast promoters via nucleosome exclusion and enhancing translation rate via removal of mRNA secondary structure.\u00a0 As a postdoc in the laboratory of Dr. Gautam Dantas at Washington University in Saint Louis, Dr. Crook explored the evolutionary trajectories of probiotic E. coli <\/em>Nissle in the mammalian gut, including genomic mutations and horizontally transferred genes. As a new assistant professor at NC State, Dr. Crook\u2019s lab combines his training in metabolic engineering and gut microbial ecology to engineer probiotic yeast (S. boulardii<\/em>) to secrete drug molecules in the gut.<\/p>\n

Relevance of Research to CGIBD Mission:<\/strong> Dr. Crook\u2019s research directly addresses the CGIBD mission of performing multidisciplinary research to reduce the burden of gastrointestinal diseases. The ability to precisely control microbial phenotypes in the complex and dynamic environment of the human gut is still in its infancy, requiring expertise in metabolic engineering, microbial ecology, and gastroenterology.\u00a0 Nevertheless, making progress toward this goal will enable precision synthesis and delivery of biologic drugs directly to the site of disease via microbial factories which are cheap to manufacture and shelf stable.<\/p>\n

CGIBD Focus Area(s):<\/strong>\u00a0 Microbiome, Probiotics<\/p>\n

Collaborators:\u00a0 <\/strong>Magness, Tonkonogy<\/p>\n

 <\/th>\n<\/tr>\n<\/tbody>\n<\/table>\n

<\/h4>\n","protected":false},"excerpt":{"rendered":"

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