{"id":9156,"date":"2025-06-12T14:10:32","date_gmt":"2025-06-12T18:10:32","guid":{"rendered":"https:\/\/www.med.unc.edu\/cgibd\/?page_id=9156"},"modified":"2025-06-12T14:55:28","modified_gmt":"2025-06-12T18:55:28","slug":"epculture-services","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/cgibd\/cores\/advanced-analytics\/epculture-services\/","title":{"rendered":"EpCulture Services"},"content":{"rendered":"<h2 data-start=\"222\" data-end=\"293\">Supporting Advanced Organoid and 2D Monolayer Models of the GI Tract<\/h2>\n<p data-start=\"295\" data-end=\"888\">The Advanced Analytics Core offers comprehensive support for culturing epithelial organoids and monolayers\u2014collectively referred to as <strong data-start=\"462\" data-end=\"476\">EpCultures<\/strong>. These in vitro models enable powerful GI research but require specialized techniques for establishment, maintenance, and analysis. Our services provide investigators with access to 3D organoids and unique 2D monolayer cultures, which offer greater flexibility. We also supply high-quality conditioned media and expert consultation to support reproducibility and innovation.<\/p>\n<hr data-start=\"890\" data-end=\"893\" \/>\n<h2 data-start=\"1465\" data-end=\"1484\"><\/h2>\n<h2 data-start=\"1465\" data-end=\"1484\">Services Offered<\/h2>\n<h3 data-start=\"1486\" data-end=\"1512\">Conditioned Media<\/h3>\n<p data-start=\"1513\" data-end=\"1569\">Custom formulations optimized for GI epithelial culture:<\/p>\n<ul data-start=\"1571\" data-end=\"1845\">\n<li data-start=\"1571\" data-end=\"1647\">\n<p data-start=\"1573\" data-end=\"1647\"><strong data-start=\"1573\" data-end=\"1588\">L-WRN Media<\/strong>: Contains L-cell-secreted Wnt3a, R-spondin 3, and Noggin<\/p>\n<\/li>\n<li data-start=\"1648\" data-end=\"1738\">\n<p data-start=\"1650\" data-end=\"1738\"><strong data-start=\"1650\" data-end=\"1669\">Expansion Media<\/strong>: Maintains intestinal stem cell populations (available on request)<\/p>\n<\/li>\n<li data-start=\"1739\" data-end=\"1845\">\n<p data-start=\"1741\" data-end=\"1845\"><strong data-start=\"1741\" data-end=\"1766\">Differentiation Media<\/strong>: For driving differentiation by removing growth factors (available upon request)<\/p>\n<\/li>\n<\/ul>\n<p><a href=\"https:\/\/www.med.unc.edu\/cgibd\/cores\/advanced-analytics\/epculture-services\/conditioned-medias-for-organoid-culture\/\">Learn more about Conditioned Media<\/a><\/p>\n<hr data-start=\"1889\" data-end=\"1892\" \/>\n<h3 data-start=\"1894\" data-end=\"1938\">Human Intestinal Stem Cells (hISCs)<\/h3>\n<p data-start=\"1939\" data-end=\"2004\">Provision of dissociated hISCs with customizable regional origin:<\/p>\n<ul data-start=\"2006\" data-end=\"2079\">\n<li data-start=\"2006\" data-end=\"2034\">\n<p data-start=\"2008\" data-end=\"2034\">Duodenum, Jejunum, Ileum<\/p>\n<\/li>\n<li data-start=\"2035\" data-end=\"2079\">\n<p data-start=\"2037\" data-end=\"2079\">Ascending, Transverse, or Descending Colon<\/p>\n<\/li>\n<\/ul>\n<hr data-start=\"2081\" data-end=\"2084\" \/>\n<h3 data-start=\"2086\" data-end=\"2120\">Multi-Well Cell Expansion<\/h3>\n<p data-start=\"2121\" data-end=\"2175\">Custom culture services for scaling EpCulture systems.<\/p>\n<ul data-start=\"2177\" data-end=\"2361\">\n<li data-start=\"2177\" data-end=\"2232\">\n<p data-start=\"2179\" data-end=\"2232\">Expansion and cryopreservation of 2D or 3D cultures<\/p>\n<\/li>\n<li data-start=\"2233\" data-end=\"2297\">\n<p data-start=\"2235\" data-end=\"2297\">Support for user-provided or AAC-sourced mouse\/human samples<\/p>\n<\/li>\n<li data-start=\"2298\" data-end=\"2361\">\n<p data-start=\"2300\" data-end=\"2361\">Plate formats: Transwells, collagen-coated plates, and others<\/p>\n<\/li>\n<\/ul>\n<hr data-start=\"2363\" data-end=\"2366\" \/>\n<h3 data-start=\"2368\" data-end=\"2410\">Organoid and Monolayer Generation<\/h3>\n<p data-start=\"2411\" data-end=\"2450\">From raw tissue to functional cultures.<\/p>\n<ul data-start=\"2452\" data-end=\"2613\">\n<li data-start=\"2452\" data-end=\"2498\">\n<p data-start=\"2454\" data-end=\"2498\">Isolation of intestinal and colonic crypts<\/p>\n<\/li>\n<li data-start=\"2499\" data-end=\"2564\">\n<p data-start=\"2501\" data-end=\"2564\">Derivation of ISCs from biopsies, resections, or mouse models<\/p>\n<\/li>\n<li data-start=\"2565\" data-end=\"2613\">\n<p data-start=\"2567\" data-end=\"2613\">Establishment of 3D organoids or 2D monolayers<\/p>\n<\/li>\n<\/ul>\n<p><a href=\"https:\/\/www.med.unc.edu\/cgibd\/cores\/advanced-analytics\/epculture-services\/epcultures\/\">Learn more about Organoid and 2d Monolayer Generation<\/a><\/p>\n<hr data-start=\"2615\" data-end=\"2618\" \/>\n<h3 data-start=\"2620\" data-end=\"2641\">Gene Editing<\/h3>\n<p data-start=\"2642\" data-end=\"2683\">Customizable genetic modifications using:<\/p>\n<ul data-start=\"2685\" data-end=\"2770\">\n<li data-start=\"2685\" data-end=\"2700\">\n<p data-start=\"2687\" data-end=\"2700\">CRISPR\/Cas9<\/p>\n<\/li>\n<li data-start=\"2701\" data-end=\"2770\">\n<p data-start=\"2703\" data-end=\"2770\">PiggyBac transposition systems<br data-start=\"2733\" data-end=\"2736\" \/><em data-start=\"2736\" data-end=\"2770\">(Available by consultation only)<\/em><\/p>\n<\/li>\n<\/ul>\n<hr data-start=\"2772\" data-end=\"2775\" \/>\n<h3 data-start=\"2777\" data-end=\"2809\">Consultation &amp; Training<\/h3>\n<p data-start=\"2810\" data-end=\"2899\">Tap into our experience to enhance your experimental design or master key lab techniques.<\/p>\n<ul data-start=\"2901\" data-end=\"3137\">\n<li data-start=\"2901\" data-end=\"2978\">\n<p data-start=\"2903\" data-end=\"2978\"><strong data-start=\"2903\" data-end=\"2920\">Consultation:<\/strong> Design support, troubleshooting, and protocol selection<\/p>\n<\/li>\n<li data-start=\"2979\" data-end=\"3137\">\n<p data-start=\"2981\" data-end=\"2996\"><strong data-start=\"2981\" data-end=\"2994\">Training:<\/strong><\/p>\n<ul data-start=\"2999\" data-end=\"3137\">\n<li data-start=\"2999\" data-end=\"3018\">\n<p data-start=\"3001\" data-end=\"3018\">ISC maintenance<\/p>\n<\/li>\n<li data-start=\"3021\" data-end=\"3055\">\n<p data-start=\"3023\" data-end=\"3055\">Passaging and cryopreservation<\/p>\n<\/li>\n<li data-start=\"3058\" data-end=\"3094\">\n<p data-start=\"3060\" data-end=\"3094\">Extracellular matrix (ECM) setup<\/p>\n<\/li>\n<li data-start=\"3097\" data-end=\"3137\">\n<p data-start=\"3099\" data-end=\"3137\">Gene editing protocols (with guidance)<\/p>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<hr data-start=\"3139\" data-end=\"3142\" \/>\n<h3 data-start=\"3144\" data-end=\"3169\">Equipment Rental<\/h3>\n<p data-start=\"3170\" data-end=\"3265\"><strong data-start=\"3170\" data-end=\"3198\">EVOM Manual TEER Machine<\/strong> available for measuring monolayer barrier integrity (manual unit).<\/p>\n<p data-start=\"3170\" data-end=\"3265\"><h2 class=\" oscitas-bootstrap-container\" > <span class=\" glyphicon glyphicon-envelope\" style=\"color:#568ebf;\"><\/span> Contact Us<\/h2>\n<p data-start=\"3170\" data-end=\"3265\">For more details or to initiate a project consultation, contact Quinn Fong at <a href=\"mailto:xequinn@med.unc.edu\">xequinn@med.unc.edu<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Supporting Advanced Organoid and 2D Monolayer Models of the GI Tract The Advanced Analytics Core offers comprehensive support for culturing epithelial organoids and monolayers\u2014collectively referred to as EpCultures. These in vitro models enable powerful GI research but require specialized techniques for establishment, maintenance, and analysis. Our services provide investigators with access to 3D organoids and &hellip; <a href=\"https:\/\/www.med.unc.edu\/cgibd\/cores\/advanced-analytics\/epculture-services\/\" aria-label=\"Read more about EpCulture Services\">Read more<\/a><\/p>\n","protected":false},"author":4366,"featured_media":0,"parent":2193,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-9156","page","type-page","status-publish","hentry","odd"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>EpCulture Services | Center for Gastrointestinal Biology and Disease<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.med.unc.edu\/cgibd\/cores\/advanced-analytics\/epculture-services\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"EpCulture Services | Center for Gastrointestinal Biology and Disease\" \/>\n<meta property=\"og:description\" content=\"Supporting Advanced Organoid and 2D Monolayer Models of the GI Tract The Advanced Analytics Core offers comprehensive support for culturing epithelial organoids and monolayers\u2014collectively referred to as EpCultures. These in vitro models enable powerful GI research but require specialized techniques for establishment, maintenance, and analysis. 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