Molecular Neuroscience

BAC recombineering, rAAV vectors, ES cell characterization, massively parallel (next generation) sequencing library preparation

Directors Instruments Services Fees Genetic Engineering Links References

The primary goal of this core is to assist neuroscientists at UNC-Chapel Hill in their efforts to molecularly manipulate and study nervous system function.  This Core is directed by Dr. JrGang Cheng, a pioneer in the field of BAC recombineering.

We offer the following services to UNC neuroscientists on a fee-for-service basis:

  • Generates transgenic and gene-targeting constructs using Bacterial Artificial Chromosome (BAC) recombineering.
  • Assists neuroscientists in generating knockin/out mice by screening gene targeted embryonic stem (ES) cells (includes 5’ and 3’ probe generation and Southern blot screening).
  • Generates expression and recombinant adenoviral (rAAV) plasmid constructs.
  • Prepares massively parallel (next-generation) sequencing libraries (RNA-seq, ChIP-seq).

Molecular Neuroscience

Location & Hours

Neuroscience Research Building
Room 8133
115 Mason Farm Road, Campus Box 7250
Chapel Hill, NC

9am-6pm M-F