{"id":5118,"date":"2023-07-01T14:08:51","date_gmt":"2023-07-01T18:08:51","guid":{"rendered":"https:\/\/www.med.unc.edu\/corefacilities\/?page_id=5118"},"modified":"2025-10-16T00:28:36","modified_gmt":"2025-10-16T04:28:36","slug":"animal-models-and-husbandry","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/corefacilities\/find-a-core-facility\/animal-models-and-husbandry\/","title":{"rendered":"Animal Models and Husbandry"},"content":{"rendered":"
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Gene Targeting<\/span><\/h2>\n

Animal Models Core<\/h3>\n

Animal Models Core <\/span><\/a>(Transgenic and knockout mice and rats) provides mouse and rat genetic modification services, reagents and related technologies such as strain rederivation and cryopreservation. Services are available to UNC and external investigators. Knockout, knock-in and conditional knockout mice are created by gene targeting in ES cells with vectors produced by the Core or provided by the client (including vectors from KOMP\/EUCOMM). The Core can also provide validation and mouse production using ES cells available from knockout consortia such as KOMP and EUCOMM. Transgenic mice and rats are produced by pronuclear injection of plasmid or BAC transgenes. Transgene cloning and BAC recombineering services are available upon request. Clients should contact the core director for information on DNA preparation for pronuclear injection. Mutant rats and mice can also be produced by pronuclear injection of Zinc Finger or TAL Effector Nucleases (ZFN\u2019s or TALEN\u2019s).<\/p>\n

Mouse Models<\/span>
\n<\/b><\/h2>\n

Animal Models Core<\/h3>\n

Animal Models Core <\/span><\/a>(Transgenic and knockout mice and rats) provides mouse and rat genetic modification services, reagents and related technologies such as strain rederivation and cryopreservation. Services are available to UNC and external investigators. Knockout, knock-in and conditional knockout mice are created by gene targeting in ES cells with vectors produced by the Core or provided by the client (including vectors from KOMP\/EUCOMM). The Core can also provide validation and mouse production using ES cells available from knockout consortia such as KOMP and EUCOMM. Transgenic mice and rats are produced by pronuclear injection of plasmid or BAC transgenes. Transgene cloning and BAC recombineering services are available upon request. Clients should contact the core director for information on DNA preparation for pronuclear injection. Mutant rats and mice can also be produced by pronuclear injection of Zinc Finger or TAL Effector Nucleases (ZFN\u2019s or TALEN\u2019s).<\/p>\n

Preclinical Research Unit<\/h3>\n

The Preclinical Research Unit<\/a> merged the Animal Studies Core with the Mouse Phase 1 Unit to provide a central facility staffed by skilled technicians to promote reproducible experimentation in laboratory animals for UNC investigators.
\nServices include small animal injection and surgical models, colony management, optical imaging, traditional therapeutic and gene therapy approaches, tumor transplantation and tumorigenicity testing, pharmacologic and pharmacodynamic assessment, experimental design and protocol assistance, and production of nu\/nu and SCID mice.<\/p>\n<\/div>\n

Gnotobiotic Core<\/h3>\n
\n

Gnotobiotic Core <\/span><\/a>(Center for Gastrointestinal Biology and Disease)
\nThe Gnotobiotic Core at The University of North Carolina at Chapel Hill maintains several inbred and outbred strains of germ-free and gnotobiotic rodents. The mission is to support animal model and basic research projects of CGIBD investigators and of scientists nationwide. Mice and rats can be purchased and shipped to investigators or experiments can be carried out in our facility.<\/p>\n

Mutant Mouse Regional Resource Center<\/h3>\n

Mutant Mouse Regional Resource Center <\/span><\/a> acts as a repository for mutant strains of mice. The MMRRC UNC center is one of four collaborating centers functioning as a single, multi-site repository and informatics center for mouse lines of interest for the research community.
\nThe centers accept strain submissions after review by a strain selection committee. The accepted strains are cryopreserved and made available worldwide, on demand, from the cryoarchive. Breeding colonies of high demand strains are established to fulfill orders.<\/p>\n

Rodent Breeding Colony Management Core<\/h3>\n

Rodent Breeding Colony Management Core<\/a> (CMC) was founded with a mission to support UNC faculty with their mouse and rat breeding needs. Our service saves researchers time, money, and covers all aspects of managing given mouse or rat colonies. No colony is too small or simple; no colony is too large or complex. We are experienced with many genetic configurations and assist in establishing the most efficient, time- and cost-effective way to breed for specific research needs. Access to a custom created shareable online database, provides us with an effective communication tool and assists with proper colony pedigree tracing . We strive to improve weaning outcomes of harder to maintain mutant strains, reduce animal stress and overproduction, and allow laboratory personnel to focus more on research related duties.\u00a0<\/span><\/span>The core staff consists of experienced AALAS-credentialed breeding specialists, a PhD-level manager, advanced manager for operations support, and an attending veterinarian faculty advisor. We currently support over 30 colonies of various sizes and steadily growing while simultaneously maintaining high expectations of the UNC community with our exemplary service.<\/span><\/span><\/p>\n

UNC Systems Genetics Core<\/h3>\n

UNC Systems Genetics Core<\/span><\/a>: The core provides Collaborative Cross mice and genotypes to investigators both at UNC Chapel Hill and at other institutions.
\nThe Collaborative Cross (CC) is a large panel of recently established recombinant inbred (RI) mouse lines specifically designed to overcome the limitations of existing genetic resources and to act as an optimal murine model of heterogeneous human populations.
\nThe goal of the program is to conduct research that results (i) in determining the function of many of the genes identified by the human genome program, (ii) in providing mouse models for genetic diseases, and (iii) makes populations of mice that mimic the human population for elucidation of Quantitative Trait Loci (QTLs).<\/p>\n

Rodent Phenotyping<\/span><\/h2>\n

Preclinical Research Unit<\/h3>\n

The Preclinical Research Unit<\/a> merged the Animal Studies Core with the Mouse Phase 1 Unit to provide a central facility staffed by skilled technicians to promote reproducible experimentation in laboratory animals for UNC investigators.
\nServices include small animal injection and surgical models, colony management, optical imaging, traditional therapeutic and gene therapy approaches, tumor transplantation and tumorigenicity testing, pharmacologic and pharmacodynamic assessment, experimental design and protocol assistance, and production of nu\/nu and SCID mice.<\/p>\n

Cardiovascular Physiology and Phenotyping Core<\/h3>\n

Cardiovascular Physiology and Phenotyping Core<\/a> provides technology, surgical skills and knowledge necessary for developing and applying preclinical mouse models that may prove to be of value in the study of human cardiovascular and other diseases. Services include: Surgical and non-surgical preclinical cardiovascular mouse models (e.g. aortic banding, myocardial infarct and hind limb ischemia moels, femoral or carotid artery injury, arterial and venous transplant, vascular access, inferior vena cava banding, etc.), Cardiovascular Phenotyping (invasive and non-invasive blood pressure measurement, small animal echocardiography, laser doppler measurements, ECG monitoring, etc.), Intravital Microscopy (Leukocyte rolling and adhesion, cell interactions, etc.), Tumor Screening.<\/p>\n

Mouse Behavioral Phenotyping Laboratory<\/span><\/h3>\n

Mouse Behavioral Phenotyping Laboratory<\/span><\/a> provides investigators within the Carolina Institiute for Developmental Disablities and throughout the University of North Carolina research community with a wide variety of mouse behavioral tasks to analyze targeted gene mutations relevant to basic research and models of human diseases, inbred strains, pharmacological treatments, gene therapies, etc.
\nInitial testing includes a standardized battery for measures of general health, home cage behavior, and neurological reflexes. Sensory abilities and motor functions are evaluated with a sequence of simple tasks. Multiple tests are available to evaluate mice in specific behavioral domains, including social interaction, learning and memory, and prepulse inhibition.<\/p>\n

Nutrition Obesity Research Center (NORC)<\/span> Animal Metabolism Phenotyping Core<\/span><\/h3>\n

The Nutrition Obesity Research Center (NORC)<\/span> Animal Metabolism Phenotyping Core<\/span><\/a> provides access to state of the art phenotyping techniques for metabolism and energy balance in mouse models of nutrition and disease. Those phenotypes are: body composition, home cage activity, energy expenditure, food\/drink consumption and behavior, voluntary\/endurance exercise capacity, bone mineral density and microbiome analysis.<\/p>\n

Pathology Service Core<\/span><\/h3>\n

The Pathology Service Core’s <\/span><\/a>clinical chemistry component assists research investigators who would greatly benefit from availability of service (on a cost-per-test basis) that replicates for animals the laboratory tests that are routine for humans. Examples include blood chemistry, urinalysis, hematology, and biomarker Immunoassay.<\/p>\n

Animal Histology<\/span><\/h2>\n

Histology Research Core Facility<\/span><\/h3>\n

The Histology Research Core Facility <\/span><\/a>serves the histology needs of the UNC community and beyond.<\/p>\n

We have experience with various organisms including, but not limited to mouse, rat, pig, cat, dog, fish, human and primate. Additionally, we have provided histology services for plants and invertebrates such as coral, caterpillars and even stinkbugs!<\/p>\n

Research applications:<\/strong>\u00a0We take pride in providing top quality paraffin and frozen sectioning, special staining, and both fluorescent and chromogenic single- and multi-label immunohistochemistry. Furthermore, we recently added in situ hybridization using RNAscope and BaseScope technology to our list of services. Our lab houses an extensive slide catalogue for our customer\u2019s use as positive and negative controls, provides access to HIER for antigen retrieval in conjunction with our IHC\/IF services, and offers the CryoJane Tape Transfer system to improve morphology during frozen sectioning.<\/p>\n

We offer all services at competitive rates with a quick turn-around time of typically within 2 weeks of sample submission. We are here to talk with you at any stage in the research process about your specific research aims and the best way to achieve the results you need.<\/p>\n

Please explore our new website where you can find detailed information about all of our services, submission instructions, answers to frequently asked questions and more.<\/p>\n

We are located in rooms 212, 213, 214, and 216 in Taylor Hall at 109 Mason Farm Rd Chapel Hill, NC 27514. We can be reached by phone at (919) 966-1202 or e-mail at hrc@med.unc.edu. Please contact us to discuss your specific research needs!<\/p>\n

Instrumentation available:\u00a0<\/strong>Leica 1950 Cryostats, LKB Rotary Microtome, LeicaRM2235 Rotary Microtome, eVos Microscope, RNAScope and HiPlex technology, CryoJane Tape Transfer System for the cryostat, Vibratome,<\/p>\n

Eligible users:<\/strong>\u00a0Any researchers at UNC or other academic institutions, and in industry.<\/p>\n

Pathology Service Core<\/span><\/h3>\n

The Pathology Service Core’s\u00a0<\/span><\/a> animal histopathology component offers consultation and training in necropsy techniques and histopathologic assays, as well as routine histology services such as processing, embedding, sectioning an H&E and special stains and spatial biology technology<\/p>\n

Research applications:<\/strong> Major services include tissue embedding and sectioning (frozen and paraffin), routine and special stains, consultation on animal study design, tissue collection, immunohistochemistry (IHC), immunofluorescence (IF), spatial biology (RNAScope, Phenocycler-Fusion, Visium, and GeoMx technologies) and pathology consultation.<\/p>\n

Instrumentation available:\u00a0<\/strong>Leica St5010 Autostainer XL, Leica CV5030 Coverslipper, NX70 Cryostat, Leica Automated Tissue Processor, ASP6025, Fluorescence Microscope E600, Leica RM2255 Automated Microtomes, Histocore Arcadia Embedder, Ventana Discovery Ultra Stainer.<\/p>\n

CGBID Histology Core<\/span><\/h3>\n

CGBID Histology Core<\/span><\/a> provides a full range of histology services, encompassing routine and specialized grossing, tissue processing, paraffin embedding, microtomy, H&E and special staining, and immunohistochemistry. Consultations are also available. CGIBD members receive priority.<\/p>\n

Animal Hematology\/Clinical Chemistry\/Immunoassays<\/h2>\n

Animal Clinical Chemistry Core<\/h3>\n

Animal Clinical Chemistry Core<\/a> provides hematological and blood chemistry testing to researchers at UNC Chapel Hill.<\/p>\n

Animal Imaging
\n<\/span><\/h2>\n

Biomedical Research Imaging Center (BRIC) Small Animal Imaging (SAI) and Center for Animal MRI (CAMRI)<\/span><\/h3>\n

Biomedical Research Imaging Center (BRIC) Small Animal Imaging (SAI)<\/span><\/a> and the Center for Animal MRI<\/a> provides various animal imaging services to facilitate biomedical research at UNC and beyond. Non-invasive in-vivo animal imaging provided by the core has been critical in many research fields including neurological study, cancer research, pharmaceutical development, etc. The goal of SAIF is to develop and offer advanced imaging technologies with state-of-the-art instrumentation and experienced staff to assist investigators in a wide range of applications in biomedical research. To date, the SAI core houses twelve major pieces of equipment for animal imaging, including two MRI, one MR\/PET, one PET\/CT, one SPECT\/CT, two microCT, three optical imaging, and two ultrasound imaging systems. The facility has become the most complete animal imaging facility regional-wise. We have expertise on imaging small animals such as rats and mice, as well as bigger animals like cat and dogs. The facility is open to all users and does accept work from external entities.<\/p>\n

Preclinical Research Unit<\/h3>\n

The Preclinical Research Unit<\/a> merged the Animal Studies Core with the Mouse Phase 1 Unit to provide a central facility staffed by skilled technicians to promote reproducible experimentation in laboratory animals for UNC investigators.
\nServices include small animal injection and surgical models, colony management, optical imaging, traditional therapeutic and gene therapy approaches, tumor transplantation and tumorigenicity testing, pharmacologic and pharmacodynamic assessment, experimental design and protocol assistance, and production of nu\/nu and SCID mice.<\/p>\n

Aquaculture Models<\/span><\/h2>\n

Zebrafish Aquaculture Core Facility<\/span><\/h3>\n

Zebrafish Aquaculture Core Facility<\/span><\/a> provides zebrafish husbandry and training services to researchers at UNC-CH. This facility operates as an IACUC Satellite Animal Facility with veterinary care provided by DLAM.
\nThe following services are available:
\n\u2022 Zebrafish embryos (several wild-type and transgenic lines available)
\n\u2022 Facilities for zebrafish microinjection and light stereomicroscopy
\n\u2022 Training for zebrafish embryo handling, microinjection, and light stereomicroscopy<\/p>\n

Large Animal Models<\/span><\/h2>\n

DCM Large Animal Studies Core<\/h3>\n

The Division of Comparative Medicine\u2019s (DCM) Large Animal Studies Core (LASC) provides comprehensive training, technical expertise, and veterinary support for both surgical and non-surgical non-rodent research. Committed to advancing scientific knowledge and improving outcomes for both animals and humans, LASC ensures all studies are conducted in alignment with ethical and regulatory standards. Our skilled technicians and veterinarians offer services including anesthesia, surgical assistance, post-operative care, biosampling, in vivo imaging, and general study conduct. To learn more, please e-mail the veterinary team<\/a>.\u00a0<\/span><\/p>\n

CGIBD Large Animal Models Core<\/span><\/h3>\n

Housed at NC State, the CGIBD Large Animal Models Core<\/a> was established because in vitro<\/em>\u00a0cell culture systems or small animal models have not always translated well to solving human health problems. The core was designed to provide models of digestive disease that are more pathophysiologically similar to humans. The large animal core has made it possible to house and rear large animals, sedate and operate them, recover and maintain them post-operatively.<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

Gene Targeting Animal Models Core Animal Models Core (Transgenic and knockout mice and rats) provides mouse and rat genetic modification services, reagents and related technologies such as strain rederivation and cryopreservation. Services are available to UNC and external investigators. 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