{"id":3764,"date":"2020-06-03T10:34:02","date_gmt":"2020-06-03T14:34:02","guid":{"rendered":"https:\/\/www.med.unc.edu\/corefacilities\/?p=3764"},"modified":"2020-06-15T12:35:41","modified_gmt":"2020-06-15T16:35:41","slug":"norc-precision-nutrition-core-offers-quantstudio-pcr-system","status":"publish","type":"post","link":"https:\/\/www.med.unc.edu\/corefacilities\/norc-precision-nutrition-core-offers-quantstudio-pcr-system\/","title":{"rendered":"NORC Precision Nutrition Core offers QuantStudio PCR System"},"content":{"rendered":"

The Precision Nutrition Core now offers the QuantStudio 12K Flex Real-Time PCR System\u2014a highly flexible, comprehensive real-time PCR platform that can genotype or quantify gene expression in a customized and targeted approach.\u00a0 The system can accommodate OpenArray plates, TaqMan\u00ae array cards, 384-well, 96-well, and FAST 96-well plates, allowing us to select the format that is right for your project for maximum flexibility and consistency with your research.
\nThe Nutrition Obesity Research Center (NORC) Precision Nutrition Core offers a suite of services that enable investigators to integrate nutrigenomics and microbiome analysis into research projects to advance our understanding of how genetics and gut microbial communities influence individual variability related to nutritional exposures and adiposity.<\/p>\n

The Precision Nutrition Core provides expertise and services in both Nutrigenomics and Microbiome Analysis.
\nCustomized, cutting-edge services include:
\n\u2022 Whole genome, exome, and targeted gene sequencing
\n\u2022 RNA-sequencing
\n\u2022 Microbiota characterization, including 16S rRNA gene sequencing and shotgun metagenomics
\n\u2022 Targeted genotyping using PCR-based assays
\n\u2022 Array-based gene expression profiling
\n\u2022 Bisulfide sequencing for methylation patterns in human and mouse models.<\/p>\n

The Core also now offers a bomb calorimetry system that includes a Parr 6200 bomb calorimeter and a Parr 6510 water handling system.
\nThe core has locations at UNC Chapel Hill and the UNC Nutrition Research Institute (NRI) campus in Kannapolis.\u00a0 Nutrigenomics services are available at the NRI campus, and microbiome services are available at both locations.<\/p>\n

Visit their website for details on services and pricing: https:\/\/sph.unc.edu\/norc\/precisionnutrition\/<\/a><\/p>\n

\"\"<\/a><\/p>\n

Attached is a typical figure generated from microbiota data. The legend is below. These data were published in: Fouladi F., Glenny, E.M., Bulik-Sullivan, E.C., Tsilimigras, M.C.B., Sioda, M., Thomas, S.A., Wang, Y., Djukic, Z., Tang, Q., Tarantino, L.M., Bulik, C.M., Fodor, A.A., Carroll, I.M. 2020 Sequence variant analysis reveals poor correlations in microbial taxonomic abundance between humans and mice after gnotobiotic transfer. ISME J. 20 PMID: 32313261. https:\/\/www.nature.com\/articles\/s41396-020-0645-z?proof=trueIn%25EF%25BB%25BF<\/p>\n

Figure 1. A) Multidimensional scaling of microbial composition of human fecal samples, slurries, and mouse fecal pellets color coded by sample type. B) Multidimensional scaling of microbial composition of human fecal samples, slurries, and mouse fecal pellets color coded by human donors. HC=healthy control; T1=patient with anorexia nervosa before clinical renourishment; T2=patient with anorexia nervosa after clinical renourishment; MDS=multidimensional scaling. Each number in the Donors legend identifies a unique donor.<\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

The Precision Nutrition Core now offers the QuantStudio 12K Flex Real-Time PCR System\u2014a highly flexible, comprehensive real-time PCR platform that can genotype or quantify gene expression in a customized and targeted approach.\u00a0 The system can accommodate OpenArray plates, TaqMan\u00ae array cards, 384-well, 96-well, and FAST 96-well plates, allowing us to select the format that is … Read more<\/a><\/p>\n","protected":false},"author":11566,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"layout":"","cellInformation":"","apiCallInformation":"","footnotes":"","_links_to":"","_links_to_target":""},"categories":[6],"tags":[3],"class_list":["post-3764","post","type-post","status-publish","format-standard","hentry","category-spotlight","tag-corespotlightstory","odd"],"acf":[],"featured_image":false,"featured_image_medium":false,"featured_image_medium_large":false,"featured_image_large":false,"featured_image_thumbnail":false,"featured_image_alt":false,"category_details":[{"name":"Spotlight","link":"https:\/\/www.med.unc.edu\/corefacilities\/category\/spotlight\/"}],"tag_details":[{"name":"CoreSpotlightStory","link":"https:\/\/www.med.unc.edu\/corefacilities\/tag\/corespotlightstory\/"}],"_links_to":[],"_links_to_target":[],"_links":{"self":[{"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/posts\/3764","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/users\/11566"}],"replies":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/comments?post=3764"}],"version-history":[{"count":0,"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/posts\/3764\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/media?parent=3764"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/categories?post=3764"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/tags?post=3764"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}