{"id":3954,"date":"2021-04-30T13:51:51","date_gmt":"2021-04-30T17:51:51","guid":{"rendered":"https:\/\/www.med.unc.edu\/corefacilities\/?p=3954"},"modified":"2021-04-30T13:52:48","modified_gmt":"2021-04-30T17:52:48","slug":"new-mass-photometer-in-unc-pep-mx-core","status":"publish","type":"post","link":"https:\/\/www.med.unc.edu\/corefacilities\/new-mass-photometer-in-unc-pep-mx-core\/","title":{"rendered":"NEW Mass Photometer in UNC PEP-MX Core"},"content":{"rendered":"<p>The <strong>UNC PEP-MX core<\/strong> has a Refeyn One <strong>Mass Photometry<\/strong> instrument that\u2019s now available for use. The Mass Photometer measures minute perturbations in the refractive index of a solution as biomolecules tumble within. Sophisticated software then calculates the masses of the particles that produced those optical differences (Figure 1).<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-3955\" src=\"https:\/\/www.med.unc.edu\/corefacilities\/wp-content\/uploads\/sites\/943\/2021\/04\/UNC-PEP-MX-CORE.png\" alt=\"UNC PEP MX CORE\" width=\"1004\" height=\"751\" srcset=\"https:\/\/www.med.unc.edu\/corefacilities\/wp-content\/uploads\/sites\/943\/2021\/04\/UNC-PEP-MX-CORE.png 1004w, https:\/\/www.med.unc.edu\/corefacilities\/wp-content\/uploads\/sites\/943\/2021\/04\/UNC-PEP-MX-CORE-300x224.png 300w, https:\/\/www.med.unc.edu\/corefacilities\/wp-content\/uploads\/sites\/943\/2021\/04\/UNC-PEP-MX-CORE-768x574.png 768w, https:\/\/www.med.unc.edu\/corefacilities\/wp-content\/uploads\/sites\/943\/2021\/04\/UNC-PEP-MX-CORE-280x210.png 280w, https:\/\/www.med.unc.edu\/corefacilities\/wp-content\/uploads\/sites\/943\/2021\/04\/UNC-PEP-MX-CORE-600x449.png 600w\" sizes=\"auto, (max-width: 1004px) 100vw, 1004px\" \/><\/p>\n<p>Figure 1. Recent experimental data acquired by the mass photometer on a joint project between the laboratories of Dr N Brown and Dr J Schisler (UNC Pharmacology). The sample was composed of the ubiquitin ligase known as CHIP (C-terminus of Hsc70-interacting protein). CHIP is known to exhibit successive oligomeric states, clearly identifiable in the histogram.<\/p>\n<p>&nbsp;<\/p>\n<p>The mass photometer has a range of 40 kDa \u2013 5 MDa with an accuracy ~5-10% of known. Sample needs are roughly 100 ul at 100 nM (~5 ug\/ml) or less, and the instrument can accommodate proteins, DNAs, RNAs, and virtually any assemblies thereof. The instrument can assess sample quality, oligomeric states, complex formation, and more. Contact the core (<a href=\"mailto:pepcore@med.unc.edu\">pepcore@med.unc.edu<\/a>) to schedule training and instrument time!<\/p>\n","protected":false},"excerpt":{"rendered":"<p>The UNC PEP-MX core has a Refeyn One Mass Photometry instrument that\u2019s now available for use.<\/p>\n","protected":false},"author":28418,"featured_media":3955,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"layout":"","cellInformation":"","apiCallInformation":"","footnotes":"","_links_to":"","_links_to_target":""},"categories":[2,6],"tags":[27],"class_list":["post-3954","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-news","category-spotlight","tag-unc-pep-mx-core","odd"],"acf":[],"featured_image":"https:\/\/www.med.unc.edu\/corefacilities\/wp-content\/uploads\/sites\/943\/2021\/04\/UNC-PEP-MX-CORE.png","featured_image_medium":"https:\/\/www.med.unc.edu\/corefacilities\/wp-content\/uploads\/sites\/943\/2021\/04\/UNC-PEP-MX-CORE-300x224.png","featured_image_medium_large":"https:\/\/www.med.unc.edu\/corefacilities\/wp-content\/uploads\/sites\/943\/2021\/04\/UNC-PEP-MX-CORE-768x574.png","featured_image_large":"https:\/\/www.med.unc.edu\/corefacilities\/wp-content\/uploads\/sites\/943\/2021\/04\/UNC-PEP-MX-CORE.png","featured_image_thumbnail":"https:\/\/www.med.unc.edu\/corefacilities\/wp-content\/uploads\/sites\/943\/2021\/04\/UNC-PEP-MX-CORE-150x150.png","featured_image_alt":"UNC PEP MX CORE","category_details":[{"name":"News","link":"https:\/\/www.med.unc.edu\/corefacilities\/category\/news\/"},{"name":"Spotlight","link":"https:\/\/www.med.unc.edu\/corefacilities\/category\/spotlight\/"}],"tag_details":[{"name":"UNC PEP-MX core","link":"https:\/\/www.med.unc.edu\/corefacilities\/tag\/unc-pep-mx-core\/"}],"_links_to":[],"_links_to_target":[],"_links":{"self":[{"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/posts\/3954","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/users\/28418"}],"replies":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/comments?post=3954"}],"version-history":[{"count":0,"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/posts\/3954\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/media\/3955"}],"wp:attachment":[{"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/media?parent=3954"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/categories?post=3954"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/tags?post=3954"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}