Caltech Cryo-EM course by Dr. Grant Jenson<\/p>\n
National Resource for Automated Molecular Microscopy (NRAMM) and the Simons Electron Microscopy Center (SEMC) at the New York Structural Biology Center videos<\/p>\n
MRC Molecular Biology Laboratory CryoEM course<\/p>\n
MYSCOPE Microscopy Training<\/strong><\/p>\n An online learning environment for electron microscopy and other imaging techniques. \u00a0Covers the theory, instrument design, applications, and practicals to test your knowledge. \u00a0Highly recommend it for people interested in learning TEM.<\/p>\n Electron Microscopy Terms<\/strong><\/p>\n Comprehensive list of microscopy terms from JEOL Ltd. \u00a0This site contains a glossary of 800 terms, with illustrations and technical information. \u00a0New terms are constantly being added or updated. \u00a0A great resource for learning and for teaching cryoEM.<\/p>\n CryoEM 101<\/strong><\/p>\n An online cryoEM learning environment in the format of a textbook. \u00a0Covers the basis of sample purification, cryo-grid preparation, screening, data collection, and image processing. \u00a0A great resource for people interested in getting started in single-particle cryoEM.<\/p>\n RELION\u00a0https:\/\/www3.mrc-lmb.cam.ac.uk\/relion\/index.php?title=Main_Page<\/a><\/p>\n CisTEM\u00a0https:\/\/cistem.org<\/a><\/p>\n CryoSPARC\u00a0https:\/\/cryosparc.com<\/a><\/p>\n EMAN2\u00a0https:\/\/blake.bcm.edu\/emanwiki\/EMAN2<\/a><\/p>\n IMOD\u00a0https:\/\/bio3d.colorado.edu\/imod\/<\/a><\/p>\n Cryodrgn https:\/\/cryodrgn.cs.princeton.edu<\/a><\/p>\n Warp\u00a0http:\/\/www.warpem.com\/warp\/<\/a><\/p>\n IsoNet https:\/\/isonetcryoet.com\/<\/a><\/p>\n Dragonfly https:\/\/dragonfly.comet.tech\/<\/a><\/p>\n Process data on a virtual machine<\/p>\n Process data on the Dogwood computer cluster<\/p>\n Single Particle LLC https:\/\/www.singleparticle.com\u00a0<\/a><\/p>\n Exxactcorp\u00a0https:\/\/www.exxactcorp.com\/Relion-for-Cryo-EM-Solutions<\/a><\/p>\n We typically use TEM grids with hole size and spacing of R1.2\/1.3 or R0.6\/1.\u00a0 We prefer to use copper or gold 300 mesh TEM grids but 200, and 400 mesh will also work fine for single-particle cryoEM.\u00a0 TEM grids with regular spaced holes can be purchased from vendors like EMS or Tedpella or direct from the manufacturers Quantifoil or Protochips.<\/p>\n Quantifoil\u00a0https:\/\/www.quantifoil.com\/<\/a><\/p>\n Protochip\u00a0https:\/\/www.protochips.com\/<\/a><\/p>\n To collect data on the UNC Talos Arctica we use the software package SerialEM.<\/p>\n SerialEM\u00a0http:\/\/bio3d.colorado.edu\/SerialEM\/<\/a><\/p>\n 20200506_SerialEM_JDS<\/a><\/p>\n <\/p>\n","protected":false},"excerpt":{"rendered":"\n
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Software for CryoEM image processing and analysis<\/h2>\n
How to process your data without a GPU Linux Workstation
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Turn-key Linux Workstations for CryoEM Application<\/h3>\n
TEM Grids for CryoEM<\/h3>\n
Software for data acquisition.<\/h3>\n
UNC CryoEM Core SerialEM Tutorial from the UNC CryoEM Journal Club<\/h3>\n
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