{"id":3233,"date":"2025-07-07T13:01:39","date_gmt":"2025-07-07T17:01:39","guid":{"rendered":"https:\/\/www.med.unc.edu\/cryo-em\/?page_id=3233"},"modified":"2026-03-03T15:03:15","modified_gmt":"2026-03-03T20:03:15","slug":"equipment","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/cryo-em\/home-2-2-2\/equipment\/","title":{"rendered":"Instruments"},"content":{"rendered":"

ThermoFisher Scientific Talos Arctica CryoTEM<\/h2>\n

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200 keV transmission electron microscope.<\/p>\n

FEG-X Gun Schottkey Emitter.<\/p>\n

Constant power C-Twin object lens with Cs = 2.7 mm.<\/p>\n

Autoloader capable of holding 12 cartridges.<\/p>\n

Ceta-D 4096 x 4096 pixel CMSO CCD.<\/p>\n

Gatan K3 5,760 x 4,092 pixel direct electron detector.<\/p>\n

Automated single-particle, electron tomography, and micro-election diffraction data collection using SerialEM or EPU-D.<\/p>\n

On-the-fly processing using cryoSPARC Live.<\/p>\n

Capable of obtaining near atomic resolution for single-particle cryoEM. Benchmarked with Apoferritin sample 1.8 \u00c5 resolution based on FSC=1.34.<\/p>\n

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Thermo Fisher Scientific Vitrobot Mark IV<\/h2>\n
\n

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Robotic cryo-plunger capable of semi-automated vitrification.<\/p>\n

Capable of manual or automated blotting.<\/p>\n

Up to 16 grids can be prepared per freezing session.<\/p>\n

Touch screen consul and foot-pedal for hands-free operation.<\/p>\n

Enclosed processing chamber that maintains high humidity (100%) and temperature (4\u201360\u00b0C) for samples.<\/p>\n

\n
\n
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Precise control of vitrification parameters including blot time.<\/p>\n

We use a mixture of ethane (40%) propane (60%) as the primary cryogen. \u00a0This much easier to use as compared to pure ethane.<\/p>\n

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Pelco easiGlow<\/h2>\n

\"\"<\/p>\n

Glow discharge cleaning system for rendering TEMs hydrophilic for CryoEM application.<\/p>\n

Easy to use and automated.<\/p>\n

Unit uses air as source of plasma.<\/p>\n

Easy to use touch panel with predefined settings.<\/p>\n

Adjustable stage glass slide holder can hold multiple grids.<\/p>\n

 <\/p>\n

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Pie Scientific Tergeo-EM plasma cleaner<\/h2>\n

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Plasma cleaner used primary for making TEM grid hydrophilic for cryo-EM applications.<\/div>\n
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Oil-free dry pump.<\/p>\n

Three gas delivery ports with precise mixing capabilities. Gases available for general use include argon, oxygen and hydrogen.<\/p>\n

Custom recipes for CryoEM applications for different types of TEM grids including (1) Quantifoil, (2) Lacey Carbon, (3) TEM grids with continuous layer of thin 2-nm carbon, (4) C-Flat, (5) UltrAufoil TEM grids.<\/p>\n<\/div>\n

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Leica EM ACE600 Sputter Coater<\/span><\/p>\n

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High vacuum coating system for carbon and sputter coating.<\/span><\/span><\/p>\n

Used to prepare ultra thin (2-4 nm) carbon for single-particle CryoEM applications.<\/p>\n

Used to prepare UltrAufoil style gold grids for single particle CryoEM application.<\/p>\n

Graphite rods and gold sputter target available for general use.<\/p>\n

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 <\/p>\n

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ThermoFisher Scientific Talos Arctica CryoTEM 200 keV transmission electron microscope. FEG-X Gun Schottkey Emitter. Constant power C-Twin object lens with Cs = 2.7 mm. Autoloader capable of holding 12 cartridges. Ceta-D 4096 x 4096 pixel CMSO CCD. Gatan K3 5,760 x 4,092 pixel direct electron detector. Automated single-particle, electron tomography, and micro-election diffraction data collection … Read more<\/a><\/p>\n","protected":false},"author":93771,"featured_media":0,"parent":2239,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-3233","page","type-page","status-publish","hentry","odd"],"acf":[],"yoast_head":"\nInstruments - Cryo EM Core<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.med.unc.edu\/cryo-em\/home-2-2-2\/equipment\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Instruments - Cryo EM Core\" \/>\n<meta property=\"og:description\" content=\"ThermoFisher Scientific Talos Arctica CryoTEM 200 keV transmission electron microscope. 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