{"id":2421,"date":"2020-05-20T13:21:10","date_gmt":"2020-05-20T17:21:10","guid":{"rendered":"https:\/\/www.med.unc.edu\/csb\/nmr\/?page_id=2421"},"modified":"2020-09-16T16:24:30","modified_gmt":"2020-09-16T20:24:30","slug":"rigor-and-reproducibility","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/csb\/nmr\/rigor-and-reproducibility\/","title":{"rendered":"Rigor and Reproducibility"},"content":{"rendered":"

Eight steps to Rigorous and Reproducible Experiments in Biomolecular Research at UNC<\/h2>\n
    \n
  1. If using a core facility,\u00a0consult\u00a0<\/strong>with the core staff in the planning stage. Consult with a statistician if you need help developing a Power Analysis to assure that your results will be adequately powered.<\/li>\n
  2. Design your experiment with\u00a0sufficient controls<\/strong>\u00a0(rigor)\u00a0and replicates <\/strong>(reproducibility).<\/li>\n
  3. Assure that ALL of your reagents (antibodies, cell lines, mice) are\u00a0fully validated <\/strong>(see below).<\/li>\n
  4. Have a clear and\u00a0detailed protoco<\/strong>l (SOP) and data analysis plan. Assure that the protocol is strictly followed or that any deviation is well documented.<\/li>\n
  5. Assure that the staff or students performing the experiment are\u00a0well trained<\/strong>\u00a0and understand each step and the importance of performing them precisely.<\/li>\n
  6. Use only\u00a0well-maintained instrumentation<\/strong>, preferably maintained and operated in a core facility with expert staff (see #1 above).<\/li>\n
  7. Document all steps<\/strong>, reagents, equipment and data analysis methods used in the experiment. Assure that the both the documentation and the data itself are properly stored in a safe data management repository.<\/li>\n
  8. Acknowledge<\/strong>\u00a0the Cancer Center Support Grant (P30 CA016086) (if applicable)<\/em>, the core (by name), and core staff in publications.<\/li>\n<\/ol>\n

    Guide to Rigor and Reproducibility for the Department of Biochemistry and Biophysics Biomolecular Nuclear Magnetic Resonance (NMR) Core Laboratory<\/h2>\n
      \n
    1. Consult\u00a0with the core staff in the planning stage. \u2013 Stuart Parnham, PhD., Director stitch@email.unc.edu<\/a>, (919)966-6184.<\/li>\n
    2. NMR sample preparation SOPs can be accessed here, please consult with NMR Core Director if your sample falls outside of these guidelines before using the instruments.<\/li>\n
    3. All New users must attend a training course with the Biomolecular NMR Director before gaining access to the Core facility and its instrumentation.<\/li>\n<\/ol>\n

      For more details on sample preparation and more common NMR experiments and applications, please scroll to the bottom of this page to access our SOPs.<\/p>\n

      Instrument Calibration Standards for all NMR Spectrometers<\/h2>\n

      Temperature calibration:
      \n<\/strong>100% Methanol-d4, 40 mm filling height.<\/p>\n

      Shim maps:
      \n<\/strong>2 mM Sucrose, 0.5 mM DSS, 2 mM NaN3<\/sub>, 10% D2<\/sub>O, 90% H2<\/sub>O and a 40 mm Filling Height.<\/p>\n

      Pulse calibrations:<\/strong><\/p>\n

      0.1 M 15<\/sup>N-Urea, 0.1 M 13<\/sup>C- CH3<\/sub>OH in 100% DMSO-d6, 40 mm filling height.<\/p>\n

      48.5 mM TPP in 100% Acetone-d6 for 31<\/sup>P \u2013 Sensitivity, 40 mm filling height.<\/p>\n

      0.05% \u03b1, \u03b1, \u03b1-F3<\/sub>-CC6<\/sub>H5<\/sub> in 100% C6<\/sub>D6<\/sub> for 19<\/sup>F \u2013 Sensitivity, 40 mm filling height.<\/p>\n

      0.8 mM 13<\/sup>C 15<\/sup>N \u2013 GB-1n 20 mM PO4<\/sub>, 100 mM NaCl, pH 6.5, 0.01% NaN3<\/sub>, 10% D2<\/sub>O, 90% H2<\/sub>O and a 40 mm filling height.<\/p>\n

      Line-shape:
      \n<\/strong>0.3% CHCl3<\/sub> in 100% Acetone-d6, 40 mm filling height.<\/p>\n

      Signal to Noise:
      \n<\/strong>0.1% Ethylbenzine in 100% CDCl3<\/sub>, 40 mm filling height.<\/p>\n

      Important Protocols<\/h2>\n

      NMR acqisition checklist Protocol<\/a><\/strong><\/p>\n

       <\/p>\n","protected":false},"excerpt":{"rendered":"

      Eight steps to Rigorous and Reproducible Experiments in Biomolecular Research at UNC If using a core facility,\u00a0consult\u00a0with the core staff in the planning stage. Consult with a statistician if you need help developing a Power Analysis to assure that your results will be adequately powered. Design your experiment with\u00a0sufficient controls\u00a0(rigor)\u00a0and replicates (reproducibility). Assure that ALL … Read more<\/a><\/p>\n","protected":false},"author":22429,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-2421","page","type-page","status-publish","hentry","odd"],"acf":[],"_links_to":[],"_links_to_target":[],"_links":{"self":[{"href":"https:\/\/www.med.unc.edu\/csb\/nmr\/wp-json\/wp\/v2\/pages\/2421","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.med.unc.edu\/csb\/nmr\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.med.unc.edu\/csb\/nmr\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/csb\/nmr\/wp-json\/wp\/v2\/users\/22429"}],"replies":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/csb\/nmr\/wp-json\/wp\/v2\/comments?post=2421"}],"version-history":[{"count":0,"href":"https:\/\/www.med.unc.edu\/csb\/nmr\/wp-json\/wp\/v2\/pages\/2421\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.med.unc.edu\/csb\/nmr\/wp-json\/wp\/v2\/media?parent=2421"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}