{"id":2236,"date":"2013-05-31T20:50:00","date_gmt":"2013-06-01T00:50:00","guid":{"rendered":"https:\/\/www.med.unc.edu\/csb\/pep\/instrumentation-1\/"},"modified":"2023-01-06T20:09:25","modified_gmt":"2023-01-07T01:09:25","slug":"instrumentation","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/csb\/pep\/instrumentation\/","title":{"rendered":"Instrumentation"},"content":{"rendered":"<p class=\" \">Please contact core <strong><a title=\"\" href=\"https:\/\/www.med.unc.edu\/csb\/pep\/contact\/\" target=\"_self\" rel=\"noopener noreferrer\">Director<\/a> <\/strong>to schedule use of Protein Expression and Purification Core Facility equipment and instrumentation.<\/p>\n<h2>General<\/h2>\n<ul>\n<li>Marketforge Sterilmatic autoclave Sorvall superspeed evolution RC centrifuge w\/ fiberlite rotors (6x1L and 8x50mls)<\/li>\n<li>Ice Machine<\/li>\n<li>2 large -80C freezers<\/li>\n<li>2 -20C freezers<\/li>\n<li>2-bay 4C refrigerator<\/li>\n<li>2 3-bay 4C refrigerators (for FPLCs)<\/li>\n<li>NanoDrop One spectrophotometer<\/li>\n<li>Gel scanner documentation system (DNA\/Protein)<\/li>\n<li>Multiple Scales, heat blocks, stir-plates, microfuges, gel rigs (DNA\/Protein), and a pH meter<\/li>\n<li>Eppendorf 96 well gradient-capable PCR Machine<\/li>\n<li>37C plate <em>E. coli<\/em> plate incubator<\/li>\n<li>Small drying oven<\/li>\n<li>Sorvall tabletop refrigerated centrifuge (ST 40R)<\/li>\n<li>Chemical fume hood<\/li>\n<\/ul>\n<h2>Protein Expression \/ Tissue Culture<\/h2>\n<ul>\n<li>2 Thermo MaxQ 8000 stackable temperature controlled shakers (<em>E. coli<\/em>)<\/li>\n<li>Benchtop temperature controlled shaker for small scale <em>E. coli<\/em> cultures<\/li>\n<li>2 Heracell 240 37C CO2 incubators<\/li>\n<li>2 Heracell 37C CO2 incubators<\/li>\n<li>1 Large Thermo Reach-in CO2 incubator outfitted with 2 Forma shakers<\/li>\n<li>4 biological safety cabinets (4ft\/6ft)<\/li>\n<li>2 Olympus CKX31 inverted microscopes<\/li>\n<li>Leica inverted microscope with fluorescence<\/li>\n<li>BioRad automated cell counters<\/li>\n<\/ul>\n<h2>Purification<\/h2>\n<ul>\n<li>Millipore Tangential Flow Concentration device<\/li>\n<li>2 Cytiva AKTAxpress FPLCs<\/li>\n<li>Cytiva Pure FPLC<\/li>\n<li>Cytiva AktaStart FPLC<\/li>\n<li>Wide variety of pre-packed and gravity flow columns with a variety of resins<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Please contact core Director to schedule use of Protein Expression and Purification Core Facility equipment and instrumentation. General Marketforge Sterilmatic autoclave Sorvall superspeed evolution RC centrifuge w\/ fiberlite rotors (6x1L and 8x50mls) Ice Machine 2 large -80C freezers 2 -20C freezers 2-bay 4C refrigerator 2 3-bay 4C refrigerators (for FPLCs) NanoDrop One spectrophotometer Gel scanner &hellip; <a href=\"https:\/\/www.med.unc.edu\/csb\/pep\/instrumentation\/\" aria-label=\"Read more about Instrumentation\">Read more<\/a><\/p>\n","protected":false},"author":22429,"featured_media":0,"parent":0,"menu_order":13,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-2236","page","type-page","status-publish","hentry","odd"],"acf":[],"_links_to":[],"_links_to_target":[],"_links":{"self":[{"href":"https:\/\/www.med.unc.edu\/csb\/pep\/wp-json\/wp\/v2\/pages\/2236","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.med.unc.edu\/csb\/pep\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.med.unc.edu\/csb\/pep\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/csb\/pep\/wp-json\/wp\/v2\/users\/22429"}],"replies":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/csb\/pep\/wp-json\/wp\/v2\/comments?post=2236"}],"version-history":[{"count":0,"href":"https:\/\/www.med.unc.edu\/csb\/pep\/wp-json\/wp\/v2\/pages\/2236\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.med.unc.edu\/csb\/pep\/wp-json\/wp\/v2\/media?parent=2236"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}