{"id":2249,"date":"2014-06-04T19:59:57","date_gmt":"2014-06-04T23:59:57","guid":{"rendered":"https:\/\/www.med.unc.edu\/csb\/pep\/services\/expression-services\/"},"modified":"2024-02-05T12:19:42","modified_gmt":"2024-02-05T17:19:42","slug":"expression-services","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/csb\/pep\/services\/expression-services\/","title":{"rendered":"Expression Services"},"content":{"rendered":"<p>The facility currently utilizes <em>E. coli<\/em> and mammalian cell expression systems. Typically, users provide a sequence-verified expression vector containing the gene(s) of interest. Alternatively, our staff can undertake expression vector construction if requested.<\/p>\n<h2><em>E. coli <\/em>Expression<\/h2>\n<ul>\n<li>Small-scale test expressions can be conducted to determine optimal expression conditions.<\/li>\n<li>Large multi-liter shaking, suspension cultures of <em>E. coli<\/em> can be grown and processed at temperatures ranging from 18\u00b0C to 42\u00b0C. Max capacity is 18L at a time.<\/li>\n<li>The facility stocks a variety of <em>E. coli<\/em> strains to address specific issues like rare-codon usage, protein misfolding, and expression in specialized media, for example, for the production of selenomethionine variants or 13C and 15N labeling.<\/li>\n<\/ul>\n<h2>Mammalian Cell Expression<\/h2>\n<ul>\n<li>Our go-to cell lines are Expi293 (HEK293) and ExpiCHO (CHO-S). Both are mammalian cell lines selected for high protein expression and dense cell growth, and both are adapted for suspension culture.<\/li>\n<li>We can culture other suspension or adherent cell lines if special circumstances are required, such as limiting glycosylation or enabling DHFR stable cell selection.<\/li>\n<li>Small-scale test expressions can be performed with virtually any suspension or adherent culture. Typically these are done for a simple &#8216;yes\/no&#8217; answer to the question of whether a protein expresses, but they can be adapted for very small scale preps.<\/li>\n<li>For large scale expression experiments in suspension culture, we have a maximum of ~15L capacity.<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>The facility currently utilizes E. coli and mammalian cell expression systems. Typically, users provide a sequence-verified expression vector containing the gene(s) of interest. Alternatively, our staff can undertake expression vector construction if requested. E. coli Expression Small-scale test expressions can be conducted to determine optimal expression conditions. Large multi-liter shaking, suspension cultures of E. coli &hellip; <a href=\"https:\/\/www.med.unc.edu\/csb\/pep\/services\/expression-services\/\" aria-label=\"Read more about Expression Services\">Read more<\/a><\/p>\n","protected":false},"author":22429,"featured_media":0,"parent":2238,"menu_order":2,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-2249","page","type-page","status-publish","hentry","odd"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Expression Services - Protein Expression and Purification<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.med.unc.edu\/csb\/pep\/services\/expression-services\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Expression Services - Protein Expression and Purification\" \/>\n<meta property=\"og:description\" content=\"The facility currently utilizes E. coli and mammalian cell expression systems. Typically, users provide a sequence-verified expression vector containing the gene(s) of interest. Alternatively, our staff can undertake expression vector construction if requested. E. coli Expression Small-scale test expressions can be conducted to determine optimal expression conditions. Large multi-liter shaking, suspension cultures of E. coli &hellip; Read more\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.med.unc.edu\/csb\/pep\/services\/expression-services\/\" \/>\n<meta property=\"og:site_name\" content=\"Protein Expression and Purification\" \/>\n<meta property=\"article:modified_time\" content=\"2024-02-05T17:19:42+00:00\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.med.unc.edu\/csb\/pep\/services\/expression-services\/\",\"url\":\"https:\/\/www.med.unc.edu\/csb\/pep\/services\/expression-services\/\",\"name\":\"Expression Services - Protein Expression and Purification\",\"isPartOf\":{\"@id\":\"https:\/\/www.med.unc.edu\/csb\/pep\/#website\"},\"datePublished\":\"2014-06-04T23:59:57+00:00\",\"dateModified\":\"2024-02-05T17:19:42+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/www.med.unc.edu\/csb\/pep\/services\/expression-services\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/www.med.unc.edu\/csb\/pep\/services\/expression-services\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/www.med.unc.edu\/csb\/pep\/services\/expression-services\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/www.med.unc.edu\/csb\/pep\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Services Overview\",\"item\":\"https:\/\/www.med.unc.edu\/csb\/pep\/services\/\"},{\"@type\":\"ListItem\",\"position\":3,\"name\":\"Expression Services\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/www.med.unc.edu\/csb\/pep\/#website\",\"url\":\"https:\/\/www.med.unc.edu\/csb\/pep\/\",\"name\":\"Protein Expression and Purification\",\"description\":\"Center for Structural Biology\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/www.med.unc.edu\/csb\/pep\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Expression Services - Protein Expression and Purification","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.med.unc.edu\/csb\/pep\/services\/expression-services\/","og_locale":"en_US","og_type":"article","og_title":"Expression Services - Protein Expression and Purification","og_description":"The facility currently utilizes E. coli and mammalian cell expression systems. Typically, users provide a sequence-verified expression vector containing the gene(s) of interest. Alternatively, our staff can undertake expression vector construction if requested. E. coli Expression Small-scale test expressions can be conducted to determine optimal expression conditions. Large multi-liter shaking, suspension cultures of E. coli &hellip; Read more","og_url":"https:\/\/www.med.unc.edu\/csb\/pep\/services\/expression-services\/","og_site_name":"Protein Expression and Purification","article_modified_time":"2024-02-05T17:19:42+00:00","twitter_card":"summary_large_image","twitter_misc":{"Est. reading time":"1 minute"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/www.med.unc.edu\/csb\/pep\/services\/expression-services\/","url":"https:\/\/www.med.unc.edu\/csb\/pep\/services\/expression-services\/","name":"Expression Services - Protein Expression and Purification","isPartOf":{"@id":"https:\/\/www.med.unc.edu\/csb\/pep\/#website"},"datePublished":"2014-06-04T23:59:57+00:00","dateModified":"2024-02-05T17:19:42+00:00","breadcrumb":{"@id":"https:\/\/www.med.unc.edu\/csb\/pep\/services\/expression-services\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.med.unc.edu\/csb\/pep\/services\/expression-services\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/www.med.unc.edu\/csb\/pep\/services\/expression-services\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/www.med.unc.edu\/csb\/pep\/"},{"@type":"ListItem","position":2,"name":"Services Overview","item":"https:\/\/www.med.unc.edu\/csb\/pep\/services\/"},{"@type":"ListItem","position":3,"name":"Expression Services"}]},{"@type":"WebSite","@id":"https:\/\/www.med.unc.edu\/csb\/pep\/#website","url":"https:\/\/www.med.unc.edu\/csb\/pep\/","name":"Protein Expression and Purification","description":"Center for Structural Biology","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/www.med.unc.edu\/csb\/pep\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"}]}},"_links_to":[],"_links_to_target":[],"_links":{"self":[{"href":"https:\/\/www.med.unc.edu\/csb\/pep\/wp-json\/wp\/v2\/pages\/2249","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.med.unc.edu\/csb\/pep\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.med.unc.edu\/csb\/pep\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/csb\/pep\/wp-json\/wp\/v2\/users\/22429"}],"replies":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/csb\/pep\/wp-json\/wp\/v2\/comments?post=2249"}],"version-history":[{"count":0,"href":"https:\/\/www.med.unc.edu\/csb\/pep\/wp-json\/wp\/v2\/pages\/2249\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/csb\/pep\/wp-json\/wp\/v2\/pages\/2238"}],"wp:attachment":[{"href":"https:\/\/www.med.unc.edu\/csb\/pep\/wp-json\/wp\/v2\/media?parent=2249"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}