{"id":2250,"date":"2013-05-31T20:50:00","date_gmt":"2013-06-01T00:50:00","guid":{"rendered":"https:\/\/www.med.unc.edu\/csb\/pep\/?page_id=2250"},"modified":"2020-09-16T17:14:15","modified_gmt":"2020-09-16T21:14:15","slug":"fees","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/csb\/pep\/services\/fees\/","title":{"rendered":"Fees"},"content":{"rendered":"<p>Every project presents its own unique challenges and milestones. Therefore we build custom packages for each client depending on their needs and scientific goals. For a cost estimate for your project please contact the facility <a class=\"internal-link\" href=\"https:\/\/www.med.unc.edu\/csb\/pep\/contact\/\">director<\/a>. Below are some example &#8216;straightforward case&#8217; component costs that can be added into a workflow, essentially, expression followed by purification.<\/p>\n<p><strong>4 L E coli expression culture; includes transformation and culture with auto or manual induction<\/strong><\/p>\n<ul>\n<li>$1090 UNC+NCSU*<\/li>\n<li>$1690 External clients<\/li>\n<\/ul>\n<p><strong>1 L Sf9 expression culture; includes bacmid generation, virus generation and amplification through P2, and expression, with location\/confirmation via Western blot<\/strong><\/p>\n<ul>\n<li>$3745 UNC+NCSU*<\/li>\n<li>$5830 External clients<\/li>\n<\/ul>\n<p><strong>1 L HEK293 expression culture; includes culture and transient transfection<\/strong><\/p>\n<ul>\n<li>$3290 UNC+NCSU*<\/li>\n<li>$5100 External clients<\/li>\n<\/ul>\n<p><strong>Two-step purification; includes cell lysis, NiNTA, SEC, QC gel, and intermediate steps, with location\/confirmation via Western blot<\/strong><\/p>\n<ul>\n<li>$1795 UNC+NCSU*<\/li>\n<li>$2790 External clients<\/li>\n<\/ul>\n<p><strong>Endotoxin testing<\/strong><\/p>\n<ul>\n<li>$125 UNC+NCSU*<\/li>\n<li>$190 External clients<\/li>\n<\/ul>\n<p><strong>(*Discounts apply for UNC LCCC members)<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Every project presents its own unique challenges and milestones. Therefore we build custom packages for each client depending on their needs and scientific goals. For a cost estimate for your project please contact the facility director. Below are some example &#8216;straightforward case&#8217; component costs that can be added into a workflow, essentially, expression followed by &hellip; <a href=\"https:\/\/www.med.unc.edu\/csb\/pep\/services\/fees\/\" aria-label=\"Read more about Fees\">Read more<\/a><\/p>\n","protected":false},"author":22429,"featured_media":0,"parent":2238,"menu_order":8,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-2250","page","type-page","status-publish","hentry","odd"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Fees - Protein Expression and Purification<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.med.unc.edu\/csb\/pep\/services\/fees\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Fees - Protein Expression and Purification\" \/>\n<meta property=\"og:description\" content=\"Every project presents its own unique challenges and milestones. Therefore we build custom packages for each client depending on their needs and scientific goals. For a cost estimate for your project please contact the facility director. 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