{"id":2250,"date":"2013-05-31T20:50:00","date_gmt":"2013-06-01T00:50:00","guid":{"rendered":"https:\/\/www.med.unc.edu\/csb\/pep\/?page_id=2250"},"modified":"2020-09-16T17:14:15","modified_gmt":"2020-09-16T21:14:15","slug":"fees","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/csb\/pep\/services\/fees\/","title":{"rendered":"Fees"},"content":{"rendered":"<p>Every project presents its own unique challenges and milestones. Therefore we build custom packages for each client depending on their needs and scientific goals. For a cost estimate for your project please contact the facility <a class=\"internal-link\" href=\"https:\/\/www.med.unc.edu\/csb\/pep\/contact\/\">director<\/a>. Below are some example &#8216;straightforward case&#8217; component costs that can be added into a workflow, essentially, expression followed by purification.<\/p>\n<p><strong>4 L E coli expression culture; includes transformation and culture with auto or manual induction<\/strong><\/p>\n<ul>\n<li>$1090 UNC+NCSU*<\/li>\n<li>$1690 External clients<\/li>\n<\/ul>\n<p><strong>1 L Sf9 expression culture; includes bacmid generation, virus generation and amplification through P2, and expression, with location\/confirmation via Western blot<\/strong><\/p>\n<ul>\n<li>$3745 UNC+NCSU*<\/li>\n<li>$5830 External clients<\/li>\n<\/ul>\n<p><strong>1 L HEK293 expression culture; includes culture and transient transfection<\/strong><\/p>\n<ul>\n<li>$3290 UNC+NCSU*<\/li>\n<li>$5100 External clients<\/li>\n<\/ul>\n<p><strong>Two-step purification; includes cell lysis, NiNTA, SEC, QC gel, and intermediate steps, with location\/confirmation via Western blot<\/strong><\/p>\n<ul>\n<li>$1795 UNC+NCSU*<\/li>\n<li>$2790 External clients<\/li>\n<\/ul>\n<p><strong>Endotoxin testing<\/strong><\/p>\n<ul>\n<li>$125 UNC+NCSU*<\/li>\n<li>$190 External clients<\/li>\n<\/ul>\n<p><strong>(*Discounts apply for UNC LCCC members)<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Every project presents its own unique challenges and milestones. Therefore we build custom packages for each client depending on their needs and scientific goals. For a cost estimate for your project please contact the facility director. Below are some example &#8216;straightforward case&#8217; component costs that can be added into a workflow, essentially, expression followed by &hellip; <a href=\"https:\/\/www.med.unc.edu\/csb\/pep\/services\/fees\/\" aria-label=\"Read more about Fees\">Read more<\/a><\/p>\n","protected":false},"author":22429,"featured_media":0,"parent":2238,"menu_order":8,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-2250","page","type-page","status-publish","hentry","odd"],"acf":[],"_links_to":[],"_links_to_target":[],"_links":{"self":[{"href":"https:\/\/www.med.unc.edu\/csb\/pep\/wp-json\/wp\/v2\/pages\/2250","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.med.unc.edu\/csb\/pep\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.med.unc.edu\/csb\/pep\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/csb\/pep\/wp-json\/wp\/v2\/users\/22429"}],"replies":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/csb\/pep\/wp-json\/wp\/v2\/comments?post=2250"}],"version-history":[{"count":0,"href":"https:\/\/www.med.unc.edu\/csb\/pep\/wp-json\/wp\/v2\/pages\/2250\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/csb\/pep\/wp-json\/wp\/v2\/pages\/2238"}],"wp:attachment":[{"href":"https:\/\/www.med.unc.edu\/csb\/pep\/wp-json\/wp\/v2\/media?parent=2250"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}