{"id":2252,"date":"2014-06-04T20:05:00","date_gmt":"2014-06-05T00:05:00","guid":{"rendered":"https:\/\/www.med.unc.edu\/csb\/pep\/services\/protein-purification\/"},"modified":"2020-09-16T17:03:01","modified_gmt":"2020-09-16T21:03:01","slug":"protein-purification","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/csb\/pep\/services\/protein-purification\/","title":{"rendered":"Protein Purification"},"content":{"rendered":"
\n

The facility has all the relevant materials, instrumentation, and expertise to accommodate all popular purification methods and systems; from basic gravity columns and dialysis to high end FPLCs and Tangential flow filtration. If users need to utilize a technique and\/or system that is not present in the facility we will work with you to try and get it working here.<\/p>\n

Fast Protein Liquid Chromatography (FPLC)<\/h2>\n

The facility is equipped with FPLCs and a wide assortment of both high- and low-grade chromatography columns, covering the vast majority of protein-purification needs (affinity capture\/ion exchange\/gel filtration). In addition, users may supply their own columns for specialized projects.<\/p>\n

AKTAxpress<\/strong> \u2013 We have 2 of these instruments. They are advanced chromatography systems designed for automated, multistep protein purification of both single and multiple samples.<\/p>\n

AKTA FPLC <\/strong>\u2013 We have one of these. It is an older workhorse system that is ideal for developing a purification protocol at small scale before transitioning to the AKTAxpress systems.<\/p>\n

Tangential Flow Filtration (TFF)<\/h2>\n

TFF is a process for the rapid concentration and dia-filtration (buffer-exchange) of large volumes of solutions, typically in preparation for further purification steps. Our system can accommodate volumes up to 10L and proteins with molecular weights of 10KDa and larger.<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

The facility has all the relevant materials, instrumentation, and expertise to accommodate all popular purification methods and systems; from basic gravity columns and dialysis to high end FPLCs and Tangential flow filtration. If users need to utilize a technique and\/or system that is not present in the facility we will work with you to try … Read more<\/a><\/p>\n","protected":false},"author":22429,"featured_media":0,"parent":2238,"menu_order":5,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-2252","page","type-page","status-publish","hentry","odd"],"acf":[],"yoast_head":"\nProtein Purification - Protein Expression and Purification<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.med.unc.edu\/csb\/pep\/services\/protein-purification\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Protein Purification - Protein Expression and Purification\" \/>\n<meta property=\"og:description\" content=\"The facility has all the relevant materials, instrumentation, and expertise to accommodate all popular purification methods and systems; from basic gravity columns and dialysis to high end FPLCs and Tangential flow filtration. If users need to utilize a technique and\/or system that is not present in the facility we will work with you to try … Read more\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.med.unc.edu\/csb\/pep\/services\/protein-purification\/\" \/>\n<meta property=\"og:site_name\" content=\"Protein Expression and Purification\" \/>\n<meta property=\"article:modified_time\" content=\"2020-09-16T21:03:01+00:00\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.med.unc.edu\/csb\/pep\/services\/protein-purification\/\",\"url\":\"https:\/\/www.med.unc.edu\/csb\/pep\/services\/protein-purification\/\",\"name\":\"Protein Purification - Protein Expression and Purification\",\"isPartOf\":{\"@id\":\"https:\/\/www.med.unc.edu\/csb\/pep\/#website\"},\"datePublished\":\"2014-06-05T00:05:00+00:00\",\"dateModified\":\"2020-09-16T21:03:01+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/www.med.unc.edu\/csb\/pep\/services\/protein-purification\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/www.med.unc.edu\/csb\/pep\/services\/protein-purification\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/www.med.unc.edu\/csb\/pep\/services\/protein-purification\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/www.med.unc.edu\/csb\/pep\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Services Overview\",\"item\":\"https:\/\/www.med.unc.edu\/csb\/pep\/services\/\"},{\"@type\":\"ListItem\",\"position\":3,\"name\":\"Protein Purification\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/www.med.unc.edu\/csb\/pep\/#website\",\"url\":\"https:\/\/www.med.unc.edu\/csb\/pep\/\",\"name\":\"Protein Expression and Purification\",\"description\":\"Center for Structural Biology\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/www.med.unc.edu\/csb\/pep\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Protein Purification - Protein Expression and Purification","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.med.unc.edu\/csb\/pep\/services\/protein-purification\/","og_locale":"en_US","og_type":"article","og_title":"Protein Purification - Protein Expression and Purification","og_description":"The facility has all the relevant materials, instrumentation, and expertise to accommodate all popular purification methods and systems; from basic gravity columns and dialysis to high end FPLCs and Tangential flow filtration. 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