{"id":2252,"date":"2014-06-04T20:05:00","date_gmt":"2014-06-05T00:05:00","guid":{"rendered":"https:\/\/www.med.unc.edu\/csb\/pep\/services\/protein-purification\/"},"modified":"2020-09-16T17:03:01","modified_gmt":"2020-09-16T21:03:01","slug":"protein-purification","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/csb\/pep\/services\/protein-purification\/","title":{"rendered":"Protein Purification"},"content":{"rendered":"<div>\n<p>The facility has all the relevant materials, instrumentation, and expertise to accommodate all popular purification methods and systems; from basic gravity columns and dialysis to high end FPLCs and Tangential flow filtration. If users need to utilize a technique and\/or system that is not present in the facility we will work with you to try and get it working here.<\/p>\n<h2>Fast Protein Liquid Chromatography (FPLC)<\/h2>\n<p>The facility is equipped with FPLCs and a wide assortment of both high- and low-grade chromatography columns, covering the vast majority of protein-purification needs (affinity capture\/ion exchange\/gel filtration). In addition, users may supply their own columns for specialized projects.<\/p>\n<p><strong>AKTAxpress<\/strong> \u2013 We have 2 of these instruments. They are advanced chromatography systems designed for automated, multistep protein purification of both single and multiple samples.<\/p>\n<p><strong>AKTA FPLC <\/strong>\u2013 We have one of these. It is an older workhorse system that is ideal for developing a purification protocol at small scale before transitioning to the AKTAxpress systems.<\/p>\n<h2>Tangential Flow Filtration (TFF)<\/h2>\n<p>TFF is a process for the rapid concentration and dia-filtration (buffer-exchange) of large volumes of solutions, typically in preparation for further purification steps. Our system can accommodate volumes up to 10L and proteins with molecular weights of 10KDa and larger.<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>The facility has all the relevant materials, instrumentation, and expertise to accommodate all popular purification methods and systems; from basic gravity columns and dialysis to high end FPLCs and Tangential flow filtration. If users need to utilize a technique and\/or system that is not present in the facility we will work with you to try &hellip; <a href=\"https:\/\/www.med.unc.edu\/csb\/pep\/services\/protein-purification\/\" aria-label=\"Read more about Protein Purification\">Read more<\/a><\/p>\n","protected":false},"author":22429,"featured_media":0,"parent":2238,"menu_order":5,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-2252","page","type-page","status-publish","hentry","odd"],"acf":[],"_links_to":[],"_links_to_target":[],"_links":{"self":[{"href":"https:\/\/www.med.unc.edu\/csb\/pep\/wp-json\/wp\/v2\/pages\/2252","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.med.unc.edu\/csb\/pep\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.med.unc.edu\/csb\/pep\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/csb\/pep\/wp-json\/wp\/v2\/users\/22429"}],"replies":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/csb\/pep\/wp-json\/wp\/v2\/comments?post=2252"}],"version-history":[{"count":0,"href":"https:\/\/www.med.unc.edu\/csb\/pep\/wp-json\/wp\/v2\/pages\/2252\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/csb\/pep\/wp-json\/wp\/v2\/pages\/2238"}],"wp:attachment":[{"href":"https:\/\/www.med.unc.edu\/csb\/pep\/wp-json\/wp\/v2\/media?parent=2252"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}