{"id":2227,"date":"2012-01-11T20:50:00","date_gmt":"2012-01-12T01:50:00","guid":{"rendered":"https:\/\/www.med.unc.edu\/csb\/unc-peptides\/facility-equipment\/"},"modified":"2026-03-17T11:17:09","modified_gmt":"2026-03-17T15:17:09","slug":"facility-equipment","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/csb\/unc-peptides\/facility-equipment\/","title":{"rendered":"Instrumentation"},"content":{"rendered":"<p class=\"lead\">The UNC HTPSA is housed in the Genetic Medicine Building and equipped for providing high quality peptide synthesis, peptide arrays and microarrays, and peptide library synthesis.<\/p>\n<div>\n<h2><strong>PTI Symphony Multiple Peptide Synthesizer<\/strong><\/h2>\n<table style=\"width: 43.2896%;height: 199px\" border=\"0\">\n<tbody>\n<tr>\n<td style=\"width: 35.2445%\">\n<p style=\"text-align: center\"><img loading=\"lazy\" decoding=\"async\" class=\"image-left alignnone wp-image-2228 size-medium\" title=\"symphony3.jpg\" src=\"https:\/\/www.med.unc.edu\/csb\/unc-peptides\/wp-content\/uploads\/sites\/906\/2018\/11\/symphony3-200x300.jpg\" alt=\"PTI Symphony Multiple Peptide Synthesizer\" width=\"200\" height=\"300\" srcset=\"https:\/\/www.med.unc.edu\/csb\/unc-peptides\/wp-content\/uploads\/sites\/906\/2018\/11\/symphony3-200x300.jpg 200w, https:\/\/www.med.unc.edu\/csb\/unc-peptides\/wp-content\/uploads\/sites\/906\/2018\/11\/symphony3-768x1153.jpg 768w, https:\/\/www.med.unc.edu\/csb\/unc-peptides\/wp-content\/uploads\/sites\/906\/2018\/11\/symphony3-682x1024.jpg 682w, https:\/\/www.med.unc.edu\/csb\/unc-peptides\/wp-content\/uploads\/sites\/906\/2018\/11\/symphony3-341x512.jpg 341w, https:\/\/www.med.unc.edu\/csb\/unc-peptides\/wp-content\/uploads\/sites\/906\/2018\/11\/symphony3.jpg 1714w\" sizes=\"auto, (max-width: 200px) 100vw, 200px\" \/><\/p>\n<\/td>\n<td style=\"width: 64.5868%\" valign=\"top\">\n<p style=\"margin: 0.25em 0pt 0.85em;line-height: 1.5em\"><span style=\"text-decoration: underline\">Specifications<\/span><\/p>\n<ul>\n<li>Parallel synthesis of up to 12 peptides<\/li>\n<li>Totally independent synthesis of each peptide<\/li>\n<li>All reagents and synthesis cycles in inert atmosphere<\/li>\n<li>Synthesis scale from 0.005 to 1.2 mmol<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<hr \/>\n<h2><strong>CEM Liberty Blue Microwave Peptide Synthesizer<\/strong><\/h2>\n<table style=\"width: 43.2896%;height: 199px\" border=\"0\">\n<tbody>\n<tr>\n<td style=\"width: 36.2563%\">\n<p style=\"text-align: center\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-2444\" src=\"https:\/\/www.med.unc.edu\/csb\/unc-peptides\/wp-content\/uploads\/sites\/906\/2019\/07\/IMG_20190117_152111903_HDR-e1563544810294-233x300.jpg\" alt=\"CEM Liberty Blue Microwave Peptide Synthesizer\" width=\"258\" height=\"331\" srcset=\"https:\/\/www.med.unc.edu\/csb\/unc-peptides\/wp-content\/uploads\/sites\/906\/2019\/07\/IMG_20190117_152111903_HDR-e1563544810294-233x300.jpg 233w, https:\/\/www.med.unc.edu\/csb\/unc-peptides\/wp-content\/uploads\/sites\/906\/2019\/07\/IMG_20190117_152111903_HDR-e1563544810294-768x989.jpg 768w, https:\/\/www.med.unc.edu\/csb\/unc-peptides\/wp-content\/uploads\/sites\/906\/2019\/07\/IMG_20190117_152111903_HDR-e1563544810294-795x1024.jpg 795w\" sizes=\"auto, (max-width: 258px) 100vw, 258px\" \/><\/p>\n<\/td>\n<td style=\"width: 63.575%\" valign=\"top\">\n<p style=\"margin: 0.25em 0pt 0.85em;line-height: 1.5em\"><span style=\"text-decoration: underline\">Specifications<\/span><\/p>\n<ul>\n<li>Fast peptide synthesis (4 min per residue)<\/li>\n<li>Totally automated synthesis of peptides containing unnatural amino acids<\/li>\n<li>Synthesis of high purity long and difficult to make peptides<\/li>\n<li>All reagents and synthesis cycles in inert atmosphere<\/li>\n<li>Synthesis scale from 0.005 to 5 mmol<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<hr \/>\n<h2><strong>PTI PS-3 Peptide Synthesizer<\/strong><\/h2>\n<table style=\"width: 44.421%;height: 163px\" border=\"0\">\n<tbody>\n<tr>\n<th style=\"width: 33.3155%\"><img loading=\"lazy\" decoding=\"async\" class=\"image-left alignnone wp-image-2229 size-medium\" style=\"border-style: none;vertical-align: middle;margin: 0.5em 1em 0.5em 0px;float: left;clear: both\" title=\"PS3.jpg\" src=\"https:\/\/www.med.unc.edu\/csb\/unc-peptides\/wp-content\/uploads\/sites\/906\/2018\/11\/PS3-300x208.jpg\" alt=\"PTI PS-3 Peptide Synthesizer\" width=\"300\" height=\"208\" srcset=\"https:\/\/www.med.unc.edu\/csb\/unc-peptides\/wp-content\/uploads\/sites\/906\/2018\/11\/PS3-300x208.jpg 300w, https:\/\/www.med.unc.edu\/csb\/unc-peptides\/wp-content\/uploads\/sites\/906\/2018\/11\/PS3-768x533.jpg 768w, https:\/\/www.med.unc.edu\/csb\/unc-peptides\/wp-content\/uploads\/sites\/906\/2018\/11\/PS3-1024x710.jpg 1024w, https:\/\/www.med.unc.edu\/csb\/unc-peptides\/wp-content\/uploads\/sites\/906\/2018\/11\/PS3-738x512.jpg 738w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/th>\n<td style=\"width: 66.5197%\" valign=\"top\">\n<p style=\"margin: 0.25em 0pt 0.85em;line-height: 1.5em;text-align: left\"><span style=\"text-decoration: underline\">Specifications<\/span><\/p>\n<ul>\n<li>Sequential synthesis up to 3 peptides<\/li>\n<li>All reagents and synthesis cycles in inert atmosphere<\/li>\n<li>Synthesis scale from 0.01 to 0.75 mmol<\/li>\n<li>Tray capacity of 45 amino acid vials<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<hr \/>\n<h2><strong>Waters 2545 preparative HPLC system<\/strong><\/h2>\n<table style=\"width: 41.8675%;height: 197px\" border=\"0\">\n<tbody>\n<tr>\n<th style=\"width: 35.7669%\"><strong><span style=\"text-decoration: underline\"><img loading=\"lazy\" decoding=\"async\" class=\"image-left alignnone wp-image-2230 size-medium\" style=\"border-style: none;vertical-align: middle;margin: 0.5em 1em 0.5em 0px;float: left;clear: both\" title=\"Waters2545_HPLC.jpg\" src=\"https:\/\/www.med.unc.edu\/csb\/unc-peptides\/wp-content\/uploads\/sites\/906\/2018\/11\/Waters2545_HPLC-300x276.jpg\" alt=\"Waters 2545 preparative HPLC system\" width=\"300\" height=\"276\" srcset=\"https:\/\/www.med.unc.edu\/csb\/unc-peptides\/wp-content\/uploads\/sites\/906\/2018\/11\/Waters2545_HPLC-300x276.jpg 300w, https:\/\/www.med.unc.edu\/csb\/unc-peptides\/wp-content\/uploads\/sites\/906\/2018\/11\/Waters2545_HPLC-557x512.jpg 557w, https:\/\/www.med.unc.edu\/csb\/unc-peptides\/wp-content\/uploads\/sites\/906\/2018\/11\/Waters2545_HPLC.jpg 662w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/span><\/strong><\/th>\n<td style=\"width: 64.2331%\" valign=\"top\"><span style=\"text-decoration: underline\">Specifications<\/span><\/p>\n<ul>\n<li>Flow rates up to 150 ml\/min<\/li>\n<li>Dual wavelength UV detector<\/li>\n<li>Injection volume up to 5 ml (or unlimited)<\/li>\n<li>Sophisticated fraction collector with several task-specific sample run modes (time, signal, peak numbers, etc.)<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<hr \/>\n<h2><strong>Waters 1525 semi-prep HPLC system<\/strong><\/h2>\n<table style=\"width: 36.5853%;height: 213px\" border=\"0\">\n<tbody>\n<tr>\n<td style=\"text-align: left;width: 45.6863%\"><img loading=\"lazy\" decoding=\"async\" class=\"image-left alignnone wp-image-2231 size-medium\" title=\"Waters1525_HPLC.jpg\" src=\"https:\/\/www.med.unc.edu\/csb\/unc-peptides\/wp-content\/uploads\/sites\/906\/2018\/11\/Waters1525_HPLC-300x281.jpg\" alt=\"Waters 1525 semi-prep HPLC system\" width=\"300\" height=\"281\" srcset=\"https:\/\/www.med.unc.edu\/csb\/unc-peptides\/wp-content\/uploads\/sites\/906\/2018\/11\/Waters1525_HPLC-300x281.jpg 300w, https:\/\/www.med.unc.edu\/csb\/unc-peptides\/wp-content\/uploads\/sites\/906\/2018\/11\/Waters1525_HPLC-546x512.jpg 546w, https:\/\/www.med.unc.edu\/csb\/unc-peptides\/wp-content\/uploads\/sites\/906\/2018\/11\/Waters1525_HPLC.jpg 693w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/td>\n<td style=\"width: 54.1177%\" valign=\"top\"><span style=\"text-decoration: underline\">Specifications<\/span><\/p>\n<ul>\n<li>Flow rates up to 22.5 ml\/min<\/li>\n<li>Photodiode Array detector<\/li>\n<li>Injection volume up to 2.5 ml<\/li>\n<li>HPLC Software controlled fraction collector<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<hr \/>\n<h2><strong>Waters Alliance analytical HPLC system<\/strong><\/h2>\n<table style=\"width: 46.4159%\" border=\"0\">\n<tbody>\n<tr>\n<th style=\"width: 35.2046%\"><img loading=\"lazy\" decoding=\"async\" class=\"image-left alignnone wp-image-2232 size-medium\" title=\"WatersAlliance_HPLC.jpg\" src=\"https:\/\/www.med.unc.edu\/csb\/unc-peptides\/wp-content\/uploads\/sites\/906\/2018\/11\/WatersAlliance_HPLC-300x295.jpg\" alt=\"Waters Alliance analytical HPLC system\" width=\"300\" height=\"295\" srcset=\"https:\/\/www.med.unc.edu\/csb\/unc-peptides\/wp-content\/uploads\/sites\/906\/2018\/11\/WatersAlliance_HPLC-300x295.jpg 300w, https:\/\/www.med.unc.edu\/csb\/unc-peptides\/wp-content\/uploads\/sites\/906\/2018\/11\/WatersAlliance_HPLC-64x64.jpg 64w, https:\/\/www.med.unc.edu\/csb\/unc-peptides\/wp-content\/uploads\/sites\/906\/2018\/11\/WatersAlliance_HPLC-520x512.jpg 520w, https:\/\/www.med.unc.edu\/csb\/unc-peptides\/wp-content\/uploads\/sites\/906\/2018\/11\/WatersAlliance_HPLC.jpg 660w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/th>\n<td style=\"width: 64.7955%\" valign=\"top\"><span style=\"text-decoration: underline\">Specifications<\/span><\/p>\n<ul>\n<li>Flow rate up 50 \u00b5l to 5 ml per minute<\/li>\n<li>Dual wavelength UV detector<\/li>\n<li>Injection volume 1 to 100 \u00b5l<\/li>\n<li>Column temperature control and autosampler<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<hr \/>\n<h2><strong>Mettler Toledo XP26 DeltaRange Microbalance<\/strong><\/h2>\n<table style=\"width: 50.3746%;height: 229px\" border=\"0\">\n<tbody>\n<tr>\n<th style=\"width: 31.5457%\"><strong><img loading=\"lazy\" decoding=\"async\" class=\"image-left alignnone wp-image-2233 size-medium\" title=\"MettlerXP26_Balance.jpg\" src=\"https:\/\/www.med.unc.edu\/csb\/unc-peptides\/wp-content\/uploads\/sites\/906\/2018\/11\/MettlerXP26_Balance-254x300.jpg\" alt=\"Mettler Toledo XP26 DeltaRange Microbalance\" width=\"254\" height=\"300\" srcset=\"https:\/\/www.med.unc.edu\/csb\/unc-peptides\/wp-content\/uploads\/sites\/906\/2018\/11\/MettlerXP26_Balance-254x300.jpg 254w, https:\/\/www.med.unc.edu\/csb\/unc-peptides\/wp-content\/uploads\/sites\/906\/2018\/11\/MettlerXP26_Balance-434x512.jpg 434w, https:\/\/www.med.unc.edu\/csb\/unc-peptides\/wp-content\/uploads\/sites\/906\/2018\/11\/MettlerXP26_Balance.jpg 572w\" sizes=\"auto, (max-width: 254px) 100vw, 254px\" \/><\/strong><\/th>\n<td style=\"width: 77.4017%\" valign=\"top\"><span style=\"text-decoration: underline\">Specifications<\/span><\/p>\n<ul>\n<li>d = 2 \u00b5g for range 0 \u2013 5.1 g<\/li>\n<li>d = 10 \u00b5g for range 5.1 \u2013 22 g<\/li>\n<li>large size of weighing pan<\/li>\n<li>electrostatic charges eliminated from samples and containers by use of high voltage U-electrode<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<hr \/>\n<h2 style=\"color: #333333\"><strong>AB Sciex 5800 MALDI-TOF\/TOF MS<\/strong><\/h2>\n<table style=\"width: 55.9457%;height: 179px\" border=\"0\">\n<tbody>\n<tr>\n<th style=\"width: 27.9475%\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-2489\" src=\"https:\/\/www.med.unc.edu\/csb\/unc-peptides\/wp-content\/uploads\/sites\/906\/2021\/11\/TOFTOF5800_mass_spec-300x300.jpg\" alt=\"AB Sciex 5800 MALDI-TOF\/TOF MS\" width=\"266\" height=\"266\" srcset=\"https:\/\/www.med.unc.edu\/csb\/unc-peptides\/wp-content\/uploads\/sites\/906\/2021\/11\/TOFTOF5800_mass_spec-300x300.jpg 300w, https:\/\/www.med.unc.edu\/csb\/unc-peptides\/wp-content\/uploads\/sites\/906\/2021\/11\/TOFTOF5800_mass_spec-150x150.jpg 150w, https:\/\/www.med.unc.edu\/csb\/unc-peptides\/wp-content\/uploads\/sites\/906\/2021\/11\/TOFTOF5800_mass_spec.jpg 409w\" sizes=\"auto, (max-width: 266px) 100vw, 266px\" \/><\/th>\n<td style=\"width: 91.6916%\" valign=\"top\"><span style=\"text-decoration: underline\">Specifications<\/span><\/p>\n<ul>\n<li>MS &amp; MS\/MS of small molecules and peptides<\/li>\n<li>0-5 kDa reflector mode (monoisotopic masses up to ~4 kDa)<\/li>\n<li>linear (low-resolution) mode for higher mass range<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<hr \/>\n<h2><strong>Two VirTis BenchTop Lyophilizers with Speed-Vac<\/strong><\/h2>\n<table style=\"width: 47.4567%\" border=\"0\">\n<tbody>\n<tr>\n<th style=\"width: 30.9783%\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone\" src=\"http:\/\/photos.labwrench.com\/equipmentPhotos\/11000\/11989-9336-m.jpg\" alt=\"Two VirTis BenchTop Lyophilizers with Speed-Vac\" width=\"150\" height=\"172\" \/><\/th>\n<td style=\"width: 86.6784%\" valign=\"top\"><span style=\"text-decoration: underline\">Specifications<\/span><\/p>\n<ul>\n<li>low temperature lyophilizers suitable to use<br \/>\nwith water\/acetonitrile mixtures<\/li>\n<li>speed-vac up to 40 samples (Eppendorf tubes) per cycle<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<hr \/>\n<table style=\"width: 56.0769%;height: 24px\" border=\"0\">\n<tbody>\n<tr>\n<th style=\"width: 35.2911%\"><\/th>\n<td style=\"width: 89.2571%\" valign=\"top\"><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>The UNC HTPSA is housed in the Genetic Medicine Building and equipped for providing high quality peptide synthesis, peptide arrays and microarrays, and peptide library synthesis. PTI Symphony Multiple Peptide Synthesizer Specifications Parallel synthesis of up to 12 peptides Totally independent synthesis of each peptide All reagents and synthesis cycles in inert atmosphere Synthesis scale &hellip; <a href=\"https:\/\/www.med.unc.edu\/csb\/unc-peptides\/facility-equipment\/\" aria-label=\"Read more about Instrumentation\">Read more<\/a><\/p>\n","protected":false},"author":22429,"featured_media":0,"parent":0,"menu_order":9,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-2227","page","type-page","status-publish","hentry","odd"],"acf":[],"_links_to":[],"_links_to_target":[],"_links":{"self":[{"href":"https:\/\/www.med.unc.edu\/csb\/unc-peptides\/wp-json\/wp\/v2\/pages\/2227","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.med.unc.edu\/csb\/unc-peptides\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.med.unc.edu\/csb\/unc-peptides\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/csb\/unc-peptides\/wp-json\/wp\/v2\/users\/22429"}],"replies":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/csb\/unc-peptides\/wp-json\/wp\/v2\/comments?post=2227"}],"version-history":[{"count":4,"href":"https:\/\/www.med.unc.edu\/csb\/unc-peptides\/wp-json\/wp\/v2\/pages\/2227\/revisions"}],"predecessor-version":[{"id":2643,"href":"https:\/\/www.med.unc.edu\/csb\/unc-peptides\/wp-json\/wp\/v2\/pages\/2227\/revisions\/2643"}],"wp:attachment":[{"href":"https:\/\/www.med.unc.edu\/csb\/unc-peptides\/wp-json\/wp\/v2\/media?parent=2227"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}