{"id":16760,"date":"2022-10-16T16:22:11","date_gmt":"2022-10-16T20:22:11","guid":{"rendered":"https:\/\/www.med.unc.edu\/fammed\/?page_id=16760"},"modified":"2022-10-16T16:23:34","modified_gmt":"2022-10-16T20:23:34","slug":"norovirus-infection-in-young-nicaraguan-children-induces-durable-and-genotype-specific-antibody-immunity","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/fammed\/research\/publications-2\/norovirus-infection-in-young-nicaraguan-children-induces-durable-and-genotype-specific-antibody-immunity\/","title":{"rendered":"Norovirus Infection in Young Nicaraguan Children Induces Durable and Genotype-Specific Antibody Immunity"},"content":{"rendered":"

Authors<\/span><\/p>\n

Paul D Brewer-Jensen,\u00a0Yaoska Reyes,\u00a0Sylvia Becker-Dreps<\/strong>,\u00a0Fredman Gonz\u00e1lez,\u00a0Michael L Mallory,\u00a0Lester Guti\u00e9rrez,\u00a0Omar Zepeda,\u00a0Edwing Centeno,\u00a0Nadja Vielot<\/strong>,\u00a0Marta Diez-Valcarce,\u00a0Jan Vinj\u00e9,\u00a0Ralph Baric,\u00a0Lisa C Lindesmith,\u00a0Filemon Bucardo<\/p>\n

Abstract<\/span><\/p>\n

There are significant challenges to the development of a pediatric norovirus vaccine, mainly due to the antigenic diversity among strains infecting young children. Characterizing human norovirus serotypes and understanding norovirus immunity in na\u00efve children would provide key information for designing rational vaccine platforms. In this study, 26 Nicaraguan children experiencing their first norovirus acute gastroenteritis (AGE) episode during the first 18 months of life were investigated. We used a surrogate neutralization assay that measured antibodies blocking the binding of 13 different norovirus virus-like particles (VLPs) to histo-blood group antigens (HBGAs) in pre- and post-infection sera. To assess for asymptomatic norovirus infections, stools from asymptomatic children were collected monthly, screened for norovirus by RT-qPCR and genotyped by sequencing. Seroconversion of an HBGA-blocking antibody matched the infecting genotype in 25 (96%) of the 26 children. A subset of 13 (50%) and 4 (15%) of the 26 children experienced monotypic GII and GI seroconversion, respectively, strongly suggesting a type-specific response in na\u00efve children, and 9 (35%) showed multitypic seroconversion. The most frequent pairing in multitypic seroconversion (8\/12) were GII.4 Sydney and GII.12 noroviruses, both co-circulating at the time. Blocking antibody titers to these two genotypes did not correlate with each other, suggesting multiple exposure rather than cross-reactivity between genotypes. In addition, GII titers remained consistent for at least 19 months post-infection, demonstrating durable immunity. In conclusion, the first natural norovirus gastroenteritis episodes in these young children were dominated by a limited number of genotypes and induced responses of antibodies blocking binding of norovirus VLPs in a genotype-specific manner, suggesting that an effective pediatric norovirus vaccine likely needs to be multivalent and include globally dominant genotypes. The duration of protection from natural infections provides optimism for pediatric norovirus vaccines administered early in life.<\/p>\n

Citation<\/span><\/p>\n

Brewer-Jensen PD, Reyes Y, Becker-Dreps S, Gonz\u00e1lez F, Mallory ML, Guti\u00e9rrez L, Zepeda O, Centeno E, Vielot N, Diez-Valcarce M, Vinj\u00e9 J, Baric R, Lindesmith LC, Bucardo F. Norovirus Infection in Young Nicaraguan Children Induces Durable and Genotype-Specific Antibody Immunity. Viruses. 2022 Sep 16;14(9):2053. doi: 10.3390\/v14092053. PMID: 36146859; PMCID: PMC9501366.<\/p>\n

Publication Link<\/span><\/p>\n

Full Text<\/span><\/a><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Authors Paul D Brewer-Jensen,\u00a0Yaoska Reyes,\u00a0Sylvia Becker-Dreps,\u00a0Fredman Gonz\u00e1lez,\u00a0Michael L Mallory,\u00a0Lester Guti\u00e9rrez,\u00a0Omar Zepeda,\u00a0Edwing Centeno,\u00a0Nadja Vielot,\u00a0Marta Diez-Valcarce,\u00a0Jan Vinj\u00e9,\u00a0Ralph Baric,\u00a0Lisa C Lindesmith,\u00a0Filemon Bucardo Abstract There are significant challenges to the development of a pediatric norovirus vaccine, mainly due to the antigenic diversity among strains infecting young children. Characterizing human norovirus serotypes and understanding norovirus immunity in na\u00efve children would … Read more<\/a><\/p>\n","protected":false},"author":63861,"featured_media":0,"parent":413,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"layout":"","cellInformation":"","apiCallInformation":"","footnotes":"","_links_to":"","_links_to_target":""},"categories":[1],"class_list":["post-16760","page","type-page","status-publish","hentry","category-news","odd"],"pp_force_visibility":null,"pp_subpost_visibility":null,"pp_inherited_force_visibility":null,"pp_inherited_subpost_visibility":null,"acf":[],"yoast_head":"\nNorovirus Infection in Young Nicaraguan Children Induces Durable and Genotype-Specific Antibody Immunity - Department of Family Medicine<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.med.unc.edu\/fammed\/research\/publications-2\/norovirus-infection-in-young-nicaraguan-children-induces-durable-and-genotype-specific-antibody-immunity\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Norovirus Infection in Young Nicaraguan Children Induces Durable and Genotype-Specific Antibody Immunity - Department of Family Medicine\" \/>\n<meta property=\"og:description\" content=\"Authors Paul D Brewer-Jensen,\u00a0Yaoska Reyes,\u00a0Sylvia Becker-Dreps,\u00a0Fredman Gonz\u00e1lez,\u00a0Michael L Mallory,\u00a0Lester Guti\u00e9rrez,\u00a0Omar Zepeda,\u00a0Edwing Centeno,\u00a0Nadja Vielot,\u00a0Marta Diez-Valcarce,\u00a0Jan Vinj\u00e9,\u00a0Ralph Baric,\u00a0Lisa C Lindesmith,\u00a0Filemon Bucardo Abstract There are significant challenges to the development of a pediatric norovirus vaccine, mainly due to the antigenic diversity among strains infecting young children. 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